Effects of <i>Bacillus amyloliquefaciens</i> XJ-BV2007 on Growth of <i>Alternaria alternata</i> and Production of Tenuazonic Acid
Large amounts of processing tomato are grown in Xinjiang, China. Tomato black spot disease, caused by <i>Alternaria</i> spp., and the produced alternaria toxins in tomato products are posing risks to human health. In this study, we isolated a rhizospheric bacterium, XJ-BV2007, from tomat...
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MDPI AG
2023-01-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/15/1/53 |
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| author | Qinlan Jia Yingying Fan Shuaishuai Duan Qiaomei Qin Yu Ding Min Yang Yan Wang Fengjuan Liu Cheng Wang |
| author_facet | Qinlan Jia Yingying Fan Shuaishuai Duan Qiaomei Qin Yu Ding Min Yang Yan Wang Fengjuan Liu Cheng Wang |
| author_sort | Qinlan Jia |
| collection | DOAJ |
| description | Large amounts of processing tomato are grown in Xinjiang, China. Tomato black spot disease, caused by <i>Alternaria</i> spp., and the produced alternaria toxins in tomato products are posing risks to human health. In this study, we isolated a rhizospheric bacterium, XJ-BV2007, from tomato (<i>Solanum lycopersicum</i>) fields, which we identified as <i>Bacillus amyloliquefaciens</i>. We found that this bacterium has a strong antagonistic effect against <i>Alternaria alternata</i> and reduces the accumulation of alternaria toxins in tomatoes. According to the antifungal activity of the bacteria-free filtrate, we revealed that <i>B. amyloliquefaciens</i> XJ-BV2007 suppresses <i>A. alternata</i> by the production of antifungal metabolites. Combining semi-preparative high-performance liquid chromatography, we employed UPLC-QTOF-MS analysis and the Oxford cup experiment to find that fengycin plays an important role in inhibiting <i>A. alternata</i>. This paper firstly reported that <i>B. amyloliquefaciens</i> efficiently controls tomato black spot disease and mycotoxins caused by <i>A. alternata</i>. <i>B. amyloliquefaciens</i> XJ-BV2007 may provide an alternative biocontrol strain for the prevention of tomato black spot disease. |
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| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-09T11:06:05Z |
| publishDate | 2023-01-01 |
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| series | Toxins |
| spelling | doaj.art-629159dd9d004ca0b3fc3b32c6db40022023-12-01T00:57:10ZengMDPI AGToxins2072-66512023-01-011515310.3390/toxins15010053Effects of <i>Bacillus amyloliquefaciens</i> XJ-BV2007 on Growth of <i>Alternaria alternata</i> and Production of Tenuazonic AcidQinlan Jia0Yingying Fan1Shuaishuai Duan2Qiaomei Qin3Yu Ding4Min Yang5Yan Wang6Fengjuan Liu7Cheng Wang8College of Life Science and Technology, Xinjiang University, Urumqi 830049, ChinaKey Laboratory of Agro-products Quality and Safety of Xinjiang, Laboratory of Quality and Safety Risk Assessment for Agri-products (Urumqi), Institute of Quality Standards & Testing Technology for Agri-products, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, ChinaCollege of Life Science and Technology, Xinjiang University, Urumqi 830049, ChinaCollege of Life Science and Technology, Xinjiang University, Urumqi 830049, ChinaCollege of Biology and Geography Sciences, Yili Normal University, Yining 835000, ChinaCollege of Biology and Geography Sciences, Yili Normal University, Yining 835000, ChinaKey Laboratory of Agro-products Quality and Safety of Xinjiang, Laboratory of Quality and Safety Risk Assessment for Agri-products (Urumqi), Institute of Quality Standards & Testing Technology for Agri-products, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, ChinaCollege of Life Science and Technology, Xinjiang University, Urumqi 830049, ChinaCollege of Life Science and Technology, Xinjiang University, Urumqi 830049, ChinaLarge amounts of processing tomato are grown in Xinjiang, China. Tomato black spot disease, caused by <i>Alternaria</i> spp., and the produced alternaria toxins in tomato products are posing risks to human health. In this study, we isolated a rhizospheric bacterium, XJ-BV2007, from tomato (<i>Solanum lycopersicum</i>) fields, which we identified as <i>Bacillus amyloliquefaciens</i>. We found that this bacterium has a strong antagonistic effect against <i>Alternaria alternata</i> and reduces the accumulation of alternaria toxins in tomatoes. According to the antifungal activity of the bacteria-free filtrate, we revealed that <i>B. amyloliquefaciens</i> XJ-BV2007 suppresses <i>A. alternata</i> by the production of antifungal metabolites. Combining semi-preparative high-performance liquid chromatography, we employed UPLC-QTOF-MS analysis and the Oxford cup experiment to find that fengycin plays an important role in inhibiting <i>A. alternata</i>. This paper firstly reported that <i>B. amyloliquefaciens</i> efficiently controls tomato black spot disease and mycotoxins caused by <i>A. alternata</i>. <i>B. amyloliquefaciens</i> XJ-BV2007 may provide an alternative biocontrol strain for the prevention of tomato black spot disease.https://www.mdpi.com/2072-6651/15/1/53<i>Alternaria alternata</i><i>Bacillus amyloliquefaciens</i>tomatotenuazonic acidlipopeptidefengycin |
| spellingShingle | Qinlan Jia Yingying Fan Shuaishuai Duan Qiaomei Qin Yu Ding Min Yang Yan Wang Fengjuan Liu Cheng Wang Effects of <i>Bacillus amyloliquefaciens</i> XJ-BV2007 on Growth of <i>Alternaria alternata</i> and Production of Tenuazonic Acid Toxins <i>Alternaria alternata</i> <i>Bacillus amyloliquefaciens</i> tomato tenuazonic acid lipopeptide fengycin |
| title | Effects of <i>Bacillus amyloliquefaciens</i> XJ-BV2007 on Growth of <i>Alternaria alternata</i> and Production of Tenuazonic Acid |
| title_full | Effects of <i>Bacillus amyloliquefaciens</i> XJ-BV2007 on Growth of <i>Alternaria alternata</i> and Production of Tenuazonic Acid |
| title_fullStr | Effects of <i>Bacillus amyloliquefaciens</i> XJ-BV2007 on Growth of <i>Alternaria alternata</i> and Production of Tenuazonic Acid |
| title_full_unstemmed | Effects of <i>Bacillus amyloliquefaciens</i> XJ-BV2007 on Growth of <i>Alternaria alternata</i> and Production of Tenuazonic Acid |
| title_short | Effects of <i>Bacillus amyloliquefaciens</i> XJ-BV2007 on Growth of <i>Alternaria alternata</i> and Production of Tenuazonic Acid |
| title_sort | effects of i bacillus amyloliquefaciens i xj bv2007 on growth of i alternaria alternata i and production of tenuazonic acid |
| topic | <i>Alternaria alternata</i> <i>Bacillus amyloliquefaciens</i> tomato tenuazonic acid lipopeptide fengycin |
| url | https://www.mdpi.com/2072-6651/15/1/53 |
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