Eave and swarm collections prove effective for biased captures of male Anopheles gambiae mosquitoes in Uganda
Abstract Background Traditional malaria vector sampling techniques bias collections towards female mosquitoes. Comprehensive understanding of vector dynamics requires balanced vector sampling of both males and females. Male mosquito sampling is also necessary for population size estimations by male-...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2021-05-01
|
| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-021-04770-x |
| _version_ | 1818647955006029824 |
|---|---|
| author | Krystal Birungi Danspaid P. Mabuka Victor Balyesima Annet Namukwaya Elinor W. Chemoges Sylvia Kiwuwa-Muyingo C. Matilda Collins Frederic Tripet Jonathan K. Kayondo |
| author_facet | Krystal Birungi Danspaid P. Mabuka Victor Balyesima Annet Namukwaya Elinor W. Chemoges Sylvia Kiwuwa-Muyingo C. Matilda Collins Frederic Tripet Jonathan K. Kayondo |
| author_sort | Krystal Birungi |
| collection | DOAJ |
| description | Abstract Background Traditional malaria vector sampling techniques bias collections towards female mosquitoes. Comprehensive understanding of vector dynamics requires balanced vector sampling of both males and females. Male mosquito sampling is also necessary for population size estimations by male-based mark-release-recapture (MRR) studies and for developing innovations in mosquito control, such as the male-targeted sterile insect technique and other genetic modification approaches. This study evaluated a range of collection methods which show promise in providing a more equal, or even male-biased, sex representation in the sample. Results Swarms were found at all study sites and were more abundant and larger at the peak of the wet season. Swarm sampling caught the most males, but when man/hour effort was factored in, sampling of eaves by aspiration was the more efficient method and also provided a representative sample of females. Grass-roofed houses were the most productive for eave collections. Overall few mosquitoes were caught with artificial resting traps (clay pots and buckets), although these sampling methods performed better at the start of the wet season than at its peak, possibly because of changes in mosquito ecology and an increased availability of natural resting sites later in the season. Aspiration of bushes was more productive at the peak of the wet season than at the start. Conclusions The results of this study demonstrate that eave aspiration was an efficient and useful male mosquito collection method at the study sites and a potentially powerful aid for swarm location and MRR studies. The methods evaluated may together deliver more sex-balanced mosquito captures and can be used in various combinations depending on the aims and ecological parameters of a given study. |
| first_indexed | 2024-12-17T01:10:45Z |
| format | Article |
| id | doaj.art-62951e0e72f440e99566dd2b7dba1c1c |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-17T01:10:45Z |
| publishDate | 2021-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-62951e0e72f440e99566dd2b7dba1c1c2022-12-21T22:09:08ZengBMCParasites & Vectors1756-33052021-05-0114111710.1186/s13071-021-04770-xEave and swarm collections prove effective for biased captures of male Anopheles gambiae mosquitoes in UgandaKrystal Birungi0Danspaid P. Mabuka1Victor Balyesima2Annet Namukwaya3Elinor W. Chemoges4Sylvia Kiwuwa-Muyingo5C. Matilda Collins6Frederic Tripet7Jonathan K. Kayondo8Entomology Division, Uganda Virus Research Institute (UVRI)Entomology Division, Uganda Virus Research Institute (UVRI)Entomology Division, Uganda Virus Research Institute (UVRI)Entomology Division, Uganda Virus Research Institute (UVRI)Entomology Division, Uganda Virus Research Institute (UVRI)MRC/UVRI & LSHTM Uganda Research UnitThe Centre for Environmental Policy, Imperial College LondonCentre for Applied Entomology and Parasitology, School of Life Sciences, Keele UniversityEntomology Division, Uganda Virus Research Institute (UVRI)Abstract Background Traditional malaria vector sampling techniques bias collections towards female mosquitoes. Comprehensive understanding of vector dynamics requires balanced vector sampling of both males and females. Male mosquito sampling is also necessary for population size estimations by male-based mark-release-recapture (MRR) studies and for developing innovations in mosquito control, such as the male-targeted sterile insect technique and other genetic modification approaches. This study evaluated a range of collection methods which show promise in providing a more equal, or even male-biased, sex representation in the sample. Results Swarms were found at all study sites and were more abundant and larger at the peak of the wet season. Swarm sampling caught the most males, but when man/hour effort was factored in, sampling of eaves by aspiration was the more efficient method and also provided a representative sample of females. Grass-roofed houses were the most productive for eave collections. Overall few mosquitoes were caught with artificial resting traps (clay pots and buckets), although these sampling methods performed better at the start of the wet season than at its peak, possibly because of changes in mosquito ecology and an increased availability of natural resting sites later in the season. Aspiration of bushes was more productive at the peak of the wet season than at the start. Conclusions The results of this study demonstrate that eave aspiration was an efficient and useful male mosquito collection method at the study sites and a potentially powerful aid for swarm location and MRR studies. The methods evaluated may together deliver more sex-balanced mosquito captures and can be used in various combinations depending on the aims and ecological parameters of a given study.https://doi.org/10.1186/s13071-021-04770-xMalariaMosquito samplingVector ecologyResting trapsSwarm samplingAspiration |
| spellingShingle | Krystal Birungi Danspaid P. Mabuka Victor Balyesima Annet Namukwaya Elinor W. Chemoges Sylvia Kiwuwa-Muyingo C. Matilda Collins Frederic Tripet Jonathan K. Kayondo Eave and swarm collections prove effective for biased captures of male Anopheles gambiae mosquitoes in Uganda Parasites & Vectors Malaria Mosquito sampling Vector ecology Resting traps Swarm sampling Aspiration |
| title | Eave and swarm collections prove effective for biased captures of male Anopheles gambiae mosquitoes in Uganda |
| title_full | Eave and swarm collections prove effective for biased captures of male Anopheles gambiae mosquitoes in Uganda |
| title_fullStr | Eave and swarm collections prove effective for biased captures of male Anopheles gambiae mosquitoes in Uganda |
| title_full_unstemmed | Eave and swarm collections prove effective for biased captures of male Anopheles gambiae mosquitoes in Uganda |
| title_short | Eave and swarm collections prove effective for biased captures of male Anopheles gambiae mosquitoes in Uganda |
| title_sort | eave and swarm collections prove effective for biased captures of male anopheles gambiae mosquitoes in uganda |
| topic | Malaria Mosquito sampling Vector ecology Resting traps Swarm sampling Aspiration |
| url | https://doi.org/10.1186/s13071-021-04770-x |
| work_keys_str_mv | AT krystalbirungi eaveandswarmcollectionsproveeffectiveforbiasedcapturesofmaleanophelesgambiaemosquitoesinuganda AT danspaidpmabuka eaveandswarmcollectionsproveeffectiveforbiasedcapturesofmaleanophelesgambiaemosquitoesinuganda AT victorbalyesima eaveandswarmcollectionsproveeffectiveforbiasedcapturesofmaleanophelesgambiaemosquitoesinuganda AT annetnamukwaya eaveandswarmcollectionsproveeffectiveforbiasedcapturesofmaleanophelesgambiaemosquitoesinuganda AT elinorwchemoges eaveandswarmcollectionsproveeffectiveforbiasedcapturesofmaleanophelesgambiaemosquitoesinuganda AT sylviakiwuwamuyingo eaveandswarmcollectionsproveeffectiveforbiasedcapturesofmaleanophelesgambiaemosquitoesinuganda AT cmatildacollins eaveandswarmcollectionsproveeffectiveforbiasedcapturesofmaleanophelesgambiaemosquitoesinuganda AT frederictripet eaveandswarmcollectionsproveeffectiveforbiasedcapturesofmaleanophelesgambiaemosquitoesinuganda AT jonathankkayondo eaveandswarmcollectionsproveeffectiveforbiasedcapturesofmaleanophelesgambiaemosquitoesinuganda |