Comparative analysis and validation of the malachite green assay for the high throughput biochemical characterization of terpene synthases

Terpenes are the largest group of natural products with important and diverse biological roles, while of tremendous economic value as fragrances, flavours and pharmaceutical agents. Class-I terpene synthases (TPSs), the dominant type of TPS enzymes, catalyze the conversion of prenyl diphosphates to...

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Bibliographic Details
Main Authors: Maria Vardakou, Melissa Salmon, Juan A. Faraldos, Paul E. O’Maille
Format: Article
Language:English
Published: Elsevier 2014-01-01
Series:MethodsX
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016114000089

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