<i>miR-377</i> Inhibits Proliferation and Differentiation of Bovine Skeletal Muscle Satellite Cells by Targeting <i>FHL2</i>
Non-coding RNAs, especially microRNAs (miRNAs), play an important role in skeletal muscle growth and development. miR-377 regulates many basic biological processes and plays a key role in tumor cell proliferation, migration and apoptosis. Nevertheless, the function of miR-377 during skeletal muscle...
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MDPI AG
2022-05-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/6/947 |
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| author | Yun Zhu Peng Li Xingang Dan Xiaolong Kang Yun Ma Yuangang Shi |
| author_facet | Yun Zhu Peng Li Xingang Dan Xiaolong Kang Yun Ma Yuangang Shi |
| author_sort | Yun Zhu |
| collection | DOAJ |
| description | Non-coding RNAs, especially microRNAs (miRNAs), play an important role in skeletal muscle growth and development. miR-377 regulates many basic biological processes and plays a key role in tumor cell proliferation, migration and apoptosis. Nevertheless, the function of miR-377 during skeletal muscle development and how it regulates skeletal muscle satellite cells (SMSCs) remains unclear. In the present study, we proposed to elucidate the regulatory mechanism of miR-377 in the proliferation and differentiation of bovine primary SMSCs. Our results showed that miR-377 can significantly inhibit the proliferation of SMSCs. In addition, we found that miR-377 can reduce myotube formation and restrain skeletal myogenic differentiation. Moreover, the results obtained from the biosynthesis and dual luciferase experiments showed that FHL2 was the target gene of miR-377. We further probed the function of FHL2 in muscle development and found that FHL2 silencing significantly suppressed the proliferation and differentiation of SMSCS, which is contrary to the role of miR-377. Furthermore, FHL2 interacts with Dishevelled-2 (Dvl2) to enable Wnt/β-catenin signaling pathway, consequently regulating skeletal muscle development. miR-377 negatively regulates the Wnt/β-catenin signaling pathway by targeting FHL2-mediated Dvl2. Overall, these findings demonstrated that miR-377 regulates the bovine SMSCs proliferation and differentiation by targeting FHL2 and attenuating the Wnt/β-catenin signaling pathway. |
| first_indexed | 2024-03-09T23:43:48Z |
| format | Article |
| id | doaj.art-6298ef5c6a9f4209ac7a13e582529ce0 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T23:43:48Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-6298ef5c6a9f4209ac7a13e582529ce02023-11-23T16:46:44ZengMDPI AGGenes2073-44252022-05-0113694710.3390/genes13060947<i>miR-377</i> Inhibits Proliferation and Differentiation of Bovine Skeletal Muscle Satellite Cells by Targeting <i>FHL2</i>Yun Zhu0Peng Li1Xingang Dan2Xiaolong Kang3Yun Ma4Yuangang Shi5School of Agriculture, Ningxia University, Helan Mountain West Road 489, Yinchuan 750021, ChinaSchool of Agriculture, Ningxia University, Helan Mountain West Road 489, Yinchuan 750021, ChinaSchool of Agriculture, Ningxia University, Helan Mountain West Road 489, Yinchuan 750021, ChinaSchool of Agriculture, Ningxia University, Helan Mountain West Road 489, Yinchuan 750021, ChinaSchool of Agriculture, Ningxia University, Helan Mountain West Road 489, Yinchuan 750021, ChinaSchool of Agriculture, Ningxia University, Helan Mountain West Road 489, Yinchuan 750021, ChinaNon-coding RNAs, especially microRNAs (miRNAs), play an important role in skeletal muscle growth and development. miR-377 regulates many basic biological processes and plays a key role in tumor cell proliferation, migration and apoptosis. Nevertheless, the function of miR-377 during skeletal muscle development and how it regulates skeletal muscle satellite cells (SMSCs) remains unclear. In the present study, we proposed to elucidate the regulatory mechanism of miR-377 in the proliferation and differentiation of bovine primary SMSCs. Our results showed that miR-377 can significantly inhibit the proliferation of SMSCs. In addition, we found that miR-377 can reduce myotube formation and restrain skeletal myogenic differentiation. Moreover, the results obtained from the biosynthesis and dual luciferase experiments showed that FHL2 was the target gene of miR-377. We further probed the function of FHL2 in muscle development and found that FHL2 silencing significantly suppressed the proliferation and differentiation of SMSCS, which is contrary to the role of miR-377. Furthermore, FHL2 interacts with Dishevelled-2 (Dvl2) to enable Wnt/β-catenin signaling pathway, consequently regulating skeletal muscle development. miR-377 negatively regulates the Wnt/β-catenin signaling pathway by targeting FHL2-mediated Dvl2. Overall, these findings demonstrated that miR-377 regulates the bovine SMSCs proliferation and differentiation by targeting FHL2 and attenuating the Wnt/β-catenin signaling pathway.https://www.mdpi.com/2073-4425/13/6/947miR-377skeletal muscle satellite cellsproliferationdifferentiationwnt/β-catenin signalingFHL2 |
| spellingShingle | Yun Zhu Peng Li Xingang Dan Xiaolong Kang Yun Ma Yuangang Shi <i>miR-377</i> Inhibits Proliferation and Differentiation of Bovine Skeletal Muscle Satellite Cells by Targeting <i>FHL2</i> Genes miR-377 skeletal muscle satellite cells proliferation differentiation wnt/β-catenin signaling FHL2 |
| title | <i>miR-377</i> Inhibits Proliferation and Differentiation of Bovine Skeletal Muscle Satellite Cells by Targeting <i>FHL2</i> |
| title_full | <i>miR-377</i> Inhibits Proliferation and Differentiation of Bovine Skeletal Muscle Satellite Cells by Targeting <i>FHL2</i> |
| title_fullStr | <i>miR-377</i> Inhibits Proliferation and Differentiation of Bovine Skeletal Muscle Satellite Cells by Targeting <i>FHL2</i> |
| title_full_unstemmed | <i>miR-377</i> Inhibits Proliferation and Differentiation of Bovine Skeletal Muscle Satellite Cells by Targeting <i>FHL2</i> |
| title_short | <i>miR-377</i> Inhibits Proliferation and Differentiation of Bovine Skeletal Muscle Satellite Cells by Targeting <i>FHL2</i> |
| title_sort | i mir 377 i inhibits proliferation and differentiation of bovine skeletal muscle satellite cells by targeting i fhl2 i |
| topic | miR-377 skeletal muscle satellite cells proliferation differentiation wnt/β-catenin signaling FHL2 |
| url | https://www.mdpi.com/2073-4425/13/6/947 |
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