Cytokinin Type-B Response Regulators Promote Bulbil Initiation in <i>Lilium lancifolium</i>
The bulbil is an important vegetative reproductive organ in triploid <i>Lilium lancifolium</i> whose development is promoted by cytokinins. Type-B response regulators (RRs) are critical regulators that mediate primary cytokinin responses and promote cytokinin-induced gene expression. How...
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MDPI AG
2021-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/7/3320 |
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| author | Guoren He Panpan Yang Yuwei Cao Yuchao Tang Ling Wang Meng Song Jing Wang Leifeng Xu Jun Ming |
| author_facet | Guoren He Panpan Yang Yuwei Cao Yuchao Tang Ling Wang Meng Song Jing Wang Leifeng Xu Jun Ming |
| author_sort | Guoren He |
| collection | DOAJ |
| description | The bulbil is an important vegetative reproductive organ in triploid <i>Lilium lancifolium</i> whose development is promoted by cytokinins. Type-B response regulators (RRs) are critical regulators that mediate primary cytokinin responses and promote cytokinin-induced gene expression. However, the function of cytokinin type-B Arabidopsis RRs (ARRs) in regulating bulbil formation is unclear. In this study, we identified five type-B <i>LlRR</i>s, <i>LlRR1</i>, <i>LlRR2</i>, <i>LlRR10</i>, <i>LlRR11</i> and <i>LlRR12</i>, in <i>L. lancifolium</i> for the first time. The five <i>LlRR</i>s encode proteins of 715, 675, 573, 582 and 647 amino acids. All of the regulators belong to the B-I subfamily, whose members typically contain a conserved CheY-homologous receiver (REC) domain and an Myb DNA-binding (MYB) domain at the N-terminus. As transcription factors, all five type-B LlRRs localize at the nucleus and are widely expressed in plant tissues, especially during axillary meristem (AM) formation. Functional analysis showed that type-B <i>LlRR</i>s are involved in bulbil formation in a functionally redundant manner and can activate <i>LlRR9</i> expression. In summary, our study elucidates the process by which cytokinins regulate bulbil initiation in <i>L. lancifolium</i> through type-B LlRRs and lays a foundation for research on the molecular mechanism of bulbil formation in the lily. |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-629c9f77b0aa47fda6610b0ff2cbb5ff2023-11-21T11:50:30ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01227332010.3390/ijms22073320Cytokinin Type-B Response Regulators Promote Bulbil Initiation in <i>Lilium lancifolium</i>Guoren He0Panpan Yang1Yuwei Cao2Yuchao Tang3Ling Wang4Meng Song5Jing Wang6Leifeng Xu7Jun Ming8Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaSchool of Foresty and Landscape Architecture, Anhui Agricultural University, Hefei 230036, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaThe bulbil is an important vegetative reproductive organ in triploid <i>Lilium lancifolium</i> whose development is promoted by cytokinins. Type-B response regulators (RRs) are critical regulators that mediate primary cytokinin responses and promote cytokinin-induced gene expression. However, the function of cytokinin type-B Arabidopsis RRs (ARRs) in regulating bulbil formation is unclear. In this study, we identified five type-B <i>LlRR</i>s, <i>LlRR1</i>, <i>LlRR2</i>, <i>LlRR10</i>, <i>LlRR11</i> and <i>LlRR12</i>, in <i>L. lancifolium</i> for the first time. The five <i>LlRR</i>s encode proteins of 715, 675, 573, 582 and 647 amino acids. All of the regulators belong to the B-I subfamily, whose members typically contain a conserved CheY-homologous receiver (REC) domain and an Myb DNA-binding (MYB) domain at the N-terminus. As transcription factors, all five type-B LlRRs localize at the nucleus and are widely expressed in plant tissues, especially during axillary meristem (AM) formation. Functional analysis showed that type-B <i>LlRR</i>s are involved in bulbil formation in a functionally redundant manner and can activate <i>LlRR9</i> expression. In summary, our study elucidates the process by which cytokinins regulate bulbil initiation in <i>L. lancifolium</i> through type-B LlRRs and lays a foundation for research on the molecular mechanism of bulbil formation in the lily.https://www.mdpi.com/1422-0067/22/7/3320<i>Lilium lancifolium</i>cytokininbulbil formationtype-B response regulators |
| spellingShingle | Guoren He Panpan Yang Yuwei Cao Yuchao Tang Ling Wang Meng Song Jing Wang Leifeng Xu Jun Ming Cytokinin Type-B Response Regulators Promote Bulbil Initiation in <i>Lilium lancifolium</i> International Journal of Molecular Sciences <i>Lilium lancifolium</i> cytokinin bulbil formation type-B response regulators |
| title | Cytokinin Type-B Response Regulators Promote Bulbil Initiation in <i>Lilium lancifolium</i> |
| title_full | Cytokinin Type-B Response Regulators Promote Bulbil Initiation in <i>Lilium lancifolium</i> |
| title_fullStr | Cytokinin Type-B Response Regulators Promote Bulbil Initiation in <i>Lilium lancifolium</i> |
| title_full_unstemmed | Cytokinin Type-B Response Regulators Promote Bulbil Initiation in <i>Lilium lancifolium</i> |
| title_short | Cytokinin Type-B Response Regulators Promote Bulbil Initiation in <i>Lilium lancifolium</i> |
| title_sort | cytokinin type b response regulators promote bulbil initiation in i lilium lancifolium i |
| topic | <i>Lilium lancifolium</i> cytokinin bulbil formation type-B response regulators |
| url | https://www.mdpi.com/1422-0067/22/7/3320 |
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