Regulation of Host Immune Response against <i>Enterobacter cloacae</i> Proteins via Computational mRNA Vaccine Design through Transcriptional Modification
<i>Enterobacter cloacae</i> is mainly responsible for sepsis, urethritis, and respiratory tract infections. These bacteria may affect the transcription of the host and particularly their immune system by producing changes in their epigenetics. In the present study, four proteins of <i...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/8/1621 |
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| author | Muhammad Naveed Khizra Jabeen Rubina Naz Muhammad Saad Mughal Ali A. Rabaan Muhammed A. Bakhrebah Fahad M. Alhoshani Mohammed Aljeldah Basim R. Al Shammari Mohammed Alissa Amal A. Sabour Rana A. Alaeq Maha A. Alshiekheid Mohammed Garout Mohammed S. Almogbel Muhammad A. Halwani Safaa A. Turkistani Naveed Ahmed |
| author_facet | Muhammad Naveed Khizra Jabeen Rubina Naz Muhammad Saad Mughal Ali A. Rabaan Muhammed A. Bakhrebah Fahad M. Alhoshani Mohammed Aljeldah Basim R. Al Shammari Mohammed Alissa Amal A. Sabour Rana A. Alaeq Maha A. Alshiekheid Mohammed Garout Mohammed S. Almogbel Muhammad A. Halwani Safaa A. Turkistani Naveed Ahmed |
| author_sort | Muhammad Naveed |
| collection | DOAJ |
| description | <i>Enterobacter cloacae</i> is mainly responsible for sepsis, urethritis, and respiratory tract infections. These bacteria may affect the transcription of the host and particularly their immune system by producing changes in their epigenetics. In the present study, four proteins of <i>Enterobacter cloacae</i> were used to predict the epitopes for the construction of an mRNA vaccine against <i>Enterobacter cloacae</i> infections. In order to generate cellular and humoral responses, various immunoinformatic-based approaches were used for developing the vaccine. The molecular docking analysis was performed for predicting the interaction among the chosen epitopes and corresponding MHC alleles. The vaccine was developed by combining epitopes (thirty-three total), which include the adjuvant Toll-like receptor-4 (TLR4). The constructed vaccine was analyzed and predicted to cover 99.2% of the global population. Additionally, in silico immunological modeling of the vaccination was also carried out. When it enters the cytoplasm of the human (host), the codon is optimized to generate the translated mRNA efficiently. Moreover, the peptide structures were analyzed and docked with TLR-3 and TLR-4. A dynamic simulation predicted the stability of the binding complex. The assumed construct was considered to be a potential candidate for a vaccine against <i>Enterobacter cloacae</i> infections. Hence, the proposed construct is suitable for in vitro analyses to validate its effectiveness. |
| first_indexed | 2024-03-09T04:03:33Z |
| format | Article |
| id | doaj.art-629effb5bc404a768a6c489928e9f0e0 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T04:03:33Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-629effb5bc404a768a6c489928e9f0e02023-12-03T14:09:32ZengMDPI AGMicroorganisms2076-26072022-08-01108162110.3390/microorganisms10081621Regulation of Host Immune Response against <i>Enterobacter cloacae</i> Proteins via Computational mRNA Vaccine Design through Transcriptional ModificationMuhammad Naveed0Khizra Jabeen1Rubina Naz2Muhammad Saad Mughal3Ali A. Rabaan4Muhammed A. Bakhrebah5Fahad M. Alhoshani6Mohammed Aljeldah7Basim R. Al Shammari8Mohammed Alissa9Amal A. Sabour10Rana A. Alaeq11Maha A. Alshiekheid12Mohammed Garout13Mohammed S. Almogbel14Muhammad A. Halwani15Safaa A. Turkistani16Naveed Ahmed17Department of Biotechnology, Faculty of Sciences and Technology, University of Central Punjab, Lahore 54590, PakistanDepartment of Biotechnology, Faculty of Sciences and Technology, University of Central Punjab, Lahore 54590, PakistanCorona Intensive Care Units, District Headquarter Teaching Hospital, Dera Ghazi Khan 33000, Punjab, PakistanDepartment of Biotechnology, Faculty of Sciences and Technology, University of Central Punjab, Lahore 54590, PakistanMolecular Diagnostic Laboratory, Johns Hopkins Aramco Healthcare, Dhahran 31311, Saudi ArabiaLife Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi ArabiaLife Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, University of Hafr Al Batin, Hafr Al Batin 39831, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, University of Hafr Al Batin, Hafr Al Batin 39831, Saudi ArabiaDepartment of Medical Laboratory Sciences, College of Applied Medical Sciences, Prince Sattam bin Abdulaziz University, Al-Kharj 11942, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Medical Laboratories Technology, Faculty of Applied Medical Science, Taibah University, Al Madinah Al Munawarh 344, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Community Medicine and Health Care for Pilgrims, Faculty of Medicine, Umm Al-Qura University, Makkah 21955, Saudi ArabiaDepartment of Medical Laboratory Sciences, College of Applied Medical Sciences, University of Hail, Hail 4030, Saudi ArabiaDepartment of Medical Microbiology, Faculty of Medicine, Al Baha University, Al Baha 4781, Saudi ArabiaDepartment of Medical Laboratory, Fakeeh College for Medical Science, Jeddah 21134, Saudi ArabiaDepartment of Biotechnology, Faculty of Sciences and Technology, University of Central Punjab, Lahore 54590, Pakistan<i>Enterobacter cloacae</i> is mainly responsible for sepsis, urethritis, and respiratory tract infections. These bacteria may affect the transcription of the host and particularly their immune system by producing changes in their epigenetics. In the present study, four proteins of <i>Enterobacter cloacae</i> were used to predict the epitopes for the construction of an mRNA vaccine against <i>Enterobacter cloacae</i> infections. In order to generate cellular and humoral responses, various immunoinformatic-based approaches were used for developing the vaccine. The molecular docking analysis was performed for predicting the interaction among the chosen epitopes and corresponding MHC alleles. The vaccine was developed by combining epitopes (thirty-three total), which include the adjuvant Toll-like receptor-4 (TLR4). The constructed vaccine was analyzed and predicted to cover 99.2% of the global population. Additionally, in silico immunological modeling of the vaccination was also carried out. When it enters the cytoplasm of the human (host), the codon is optimized to generate the translated mRNA efficiently. Moreover, the peptide structures were analyzed and docked with TLR-3 and TLR-4. A dynamic simulation predicted the stability of the binding complex. The assumed construct was considered to be a potential candidate for a vaccine against <i>Enterobacter cloacae</i> infections. Hence, the proposed construct is suitable for in vitro analyses to validate its effectiveness.https://www.mdpi.com/2076-2607/10/8/1621immunoinformaticin silicobioinformaticsantibiotic resistanceAMR |
| spellingShingle | Muhammad Naveed Khizra Jabeen Rubina Naz Muhammad Saad Mughal Ali A. Rabaan Muhammed A. Bakhrebah Fahad M. Alhoshani Mohammed Aljeldah Basim R. Al Shammari Mohammed Alissa Amal A. Sabour Rana A. Alaeq Maha A. Alshiekheid Mohammed Garout Mohammed S. Almogbel Muhammad A. Halwani Safaa A. Turkistani Naveed Ahmed Regulation of Host Immune Response against <i>Enterobacter cloacae</i> Proteins via Computational mRNA Vaccine Design through Transcriptional Modification Microorganisms immunoinformatic in silico bioinformatics antibiotic resistance AMR |
| title | Regulation of Host Immune Response against <i>Enterobacter cloacae</i> Proteins via Computational mRNA Vaccine Design through Transcriptional Modification |
| title_full | Regulation of Host Immune Response against <i>Enterobacter cloacae</i> Proteins via Computational mRNA Vaccine Design through Transcriptional Modification |
| title_fullStr | Regulation of Host Immune Response against <i>Enterobacter cloacae</i> Proteins via Computational mRNA Vaccine Design through Transcriptional Modification |
| title_full_unstemmed | Regulation of Host Immune Response against <i>Enterobacter cloacae</i> Proteins via Computational mRNA Vaccine Design through Transcriptional Modification |
| title_short | Regulation of Host Immune Response against <i>Enterobacter cloacae</i> Proteins via Computational mRNA Vaccine Design through Transcriptional Modification |
| title_sort | regulation of host immune response against i enterobacter cloacae i proteins via computational mrna vaccine design through transcriptional modification |
| topic | immunoinformatic in silico bioinformatics antibiotic resistance AMR |
| url | https://www.mdpi.com/2076-2607/10/8/1621 |
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