BIOTRANSFORMATION OF METRONIDAZOLE BY CUNNINGHAMELLA ELEGANS ATCC 9245
Drug biotransformation studies appear as an alternative to pharmacological studies of metabolites, development of new drug candidates with reduced investment as well as the most efficient production of chemical structures involves and drug quality control studies. A wide range of reactions in biotra...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal do Rio Grande do Sul
2019-07-01
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| Series: | Drug Analytical Research |
| Subjects: | |
| Online Access: | https://seer.ufrgs.br/dar/article/view/94032/53386 |
| _version_ | 1818737611507761152 |
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| author | Julia Medeiros Sorrentino Rafaela Martins Sponchiado Natália Olegário dos Santos Sendy Salles Oliveira Karina Galle Cassia Virginia Garcia Alexandre Meneghello Fuentefria Martin Steppe |
| author_facet | Julia Medeiros Sorrentino Rafaela Martins Sponchiado Natália Olegário dos Santos Sendy Salles Oliveira Karina Galle Cassia Virginia Garcia Alexandre Meneghello Fuentefria Martin Steppe |
| author_sort | Julia Medeiros Sorrentino |
| collection | DOAJ |
| description | Drug biotransformation studies appear as an alternative to pharmacological studies of metabolites, development of new drug candidates with reduced investment as well as the most efficient production of chemical structures involves and drug quality control studies. A wide range of reactions in biotransformations process is catalyzed by microorganisms. Fungi can be considered as a promising source of new biotransformation reactions. The aim of this study was to evaluate the capacity of metronidazole biotransformation through the filamentous fungus Cunninghamella elegans ATCC 9245. The monitoring of metabolite formation was performed by high-performance liquid chromatography (HPLC) coupled to ultraviolet (UV) spectrophotometry. The results of the biotransformation of metronidazole showed drug consumption in culture and the formation of four new chromatographic peaks of chemical structures not elucidated. The method showed it became linear over 10-70 μg/mL (r = 0.999953). Accuracy, precision and stability studies agree with international guidelines. Results are consistent in accordance with the principles of green chemistry as the experimental conditions had a low environmental impact, and few solvents use. |
| first_indexed | 2024-12-18T00:55:48Z |
| format | Article |
| id | doaj.art-629feabe43c24de599346b2e03f2c662 |
| institution | Directory Open Access Journal |
| issn | 2527-2616 |
| language | English |
| last_indexed | 2024-12-18T00:55:48Z |
| publishDate | 2019-07-01 |
| publisher | Universidade Federal do Rio Grande do Sul |
| record_format | Article |
| series | Drug Analytical Research |
| spelling | doaj.art-629feabe43c24de599346b2e03f2c6622022-12-21T21:26:31ZengUniversidade Federal do Rio Grande do SulDrug Analytical Research2527-26162019-07-01313641https://doi.org/10.22456/2527-2616.94032BIOTRANSFORMATION OF METRONIDAZOLE BY CUNNINGHAMELLA ELEGANS ATCC 9245Julia Medeiros Sorrentino0Rafaela Martins Sponchiado1Natália Olegário dos Santos2Sendy Salles Oliveira3Karina Galle4Cassia Virginia Garcia5Alexandre Meneghello Fuentefria6Martin Steppe7Universidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulDrug biotransformation studies appear as an alternative to pharmacological studies of metabolites, development of new drug candidates with reduced investment as well as the most efficient production of chemical structures involves and drug quality control studies. A wide range of reactions in biotransformations process is catalyzed by microorganisms. Fungi can be considered as a promising source of new biotransformation reactions. The aim of this study was to evaluate the capacity of metronidazole biotransformation through the filamentous fungus Cunninghamella elegans ATCC 9245. The monitoring of metabolite formation was performed by high-performance liquid chromatography (HPLC) coupled to ultraviolet (UV) spectrophotometry. The results of the biotransformation of metronidazole showed drug consumption in culture and the formation of four new chromatographic peaks of chemical structures not elucidated. The method showed it became linear over 10-70 μg/mL (r = 0.999953). Accuracy, precision and stability studies agree with international guidelines. Results are consistent in accordance with the principles of green chemistry as the experimental conditions had a low environmental impact, and few solvents use.https://seer.ufrgs.br/dar/article/view/94032/53386biotransformationhplcmetabolitesmetronidazole |
| spellingShingle | Julia Medeiros Sorrentino Rafaela Martins Sponchiado Natália Olegário dos Santos Sendy Salles Oliveira Karina Galle Cassia Virginia Garcia Alexandre Meneghello Fuentefria Martin Steppe BIOTRANSFORMATION OF METRONIDAZOLE BY CUNNINGHAMELLA ELEGANS ATCC 9245 Drug Analytical Research biotransformation hplc metabolites metronidazole |
| title | BIOTRANSFORMATION OF METRONIDAZOLE BY CUNNINGHAMELLA ELEGANS ATCC 9245 |
| title_full | BIOTRANSFORMATION OF METRONIDAZOLE BY CUNNINGHAMELLA ELEGANS ATCC 9245 |
| title_fullStr | BIOTRANSFORMATION OF METRONIDAZOLE BY CUNNINGHAMELLA ELEGANS ATCC 9245 |
| title_full_unstemmed | BIOTRANSFORMATION OF METRONIDAZOLE BY CUNNINGHAMELLA ELEGANS ATCC 9245 |
| title_short | BIOTRANSFORMATION OF METRONIDAZOLE BY CUNNINGHAMELLA ELEGANS ATCC 9245 |
| title_sort | biotransformation of metronidazole by cunninghamella elegans atcc 9245 |
| topic | biotransformation hplc metabolites metronidazole |
| url | https://seer.ufrgs.br/dar/article/view/94032/53386 |
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