Development of a Highly Sensitive Nested PCR and Its Application for the Diagnosis of Cutaneous Leishmaniasis in Sri Lanka

Development of a Highly Sensitive Nested PCR and Its Application for the Diagnosis of Cutaneous Leishmaniasis in Sri Lanka

The recent surge in cutaneous leishmaniasis (CL) in Sri Lanka has rendered clinical diagnosis difficult; thus, laboratory confirmation is indispensable. A modified (two novel inner primers to detect CL caused by <i>Leishmania donovani</i>) nested Internal Transcribed Spacer-1 (ITS1) PCR-...

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Bibliographic Details
Main Authors: Nirmitha Lalindi De Silva, Viraji Nefertiti Hiromel De Silva, Arachchige Theja Hemapala Deerasinghe, Upeksha Lakmini Rathnapala, Makoto Itoh, Hidekazu Takagi, Mirani Vasanthamala Weerasooriya, Hirotomo Kato, Thishan Channa Yahathugoda
Format: Article
Language:English
Published: MDPI AG 2022-05-01
Series:Microorganisms
Subjects:
cutaneous leishmaniasis
ITS
PCR-RFLP
Sri Lanka
Online Access:https://www.mdpi.com/2076-2607/10/5/990

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