Determination of Indolepropionic Acid and Related Indoles in Plasma, Plasma Ultrafiltrate, and Saliva
The microbial metabolite indolepropionic acid (IPA) and related indolic metabolites, including indolecarboxylic acid (ICA), indolelactic acid (ILA), indoleacetic acid (IAA), indolebutyric acid (IBA), indoxylsulfate (ISO4), and indole, were determined in human plasma, plasma ultrafiltrate (UF), and s...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/13/5/602 |
| _version_ | 1797599127998038016 |
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| author | George M. Anderson |
| author_facet | George M. Anderson |
| author_sort | George M. Anderson |
| collection | DOAJ |
| description | The microbial metabolite indolepropionic acid (IPA) and related indolic metabolites, including indolecarboxylic acid (ICA), indolelactic acid (ILA), indoleacetic acid (IAA), indolebutyric acid (IBA), indoxylsulfate (ISO4), and indole, were determined in human plasma, plasma ultrafiltrate (UF), and saliva. The compounds were separated on a 150 × 3 mm column of 3 μm Hypersil C18 eluted with a mobile phase of 80% pH 5 0.01 M sodium acetate containing 1.0 g/L of tert-butylammonium chloride/20% acetonitrile and then detected fluorometrically. Levels of IPA in human plasma UF and of ILA in saliva are reported for the first time. The determination of IPA in plasma UF enables the first report of free plasma IPA, the presumed physiologically active pool of this important microbial metabolite of tryptophan. Plasma and salivary ICA and IBA were not detected, consistent with the absence of any prior reported values. Observed levels or limits of detection for other indolic metabolites usefully supplement limited prior reports. |
| first_indexed | 2024-03-11T03:30:20Z |
| format | Article |
| id | doaj.art-62aa83a93dc34fa5a289b7b43f1dcf0b |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-11T03:30:20Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-62aa83a93dc34fa5a289b7b43f1dcf0b2023-11-18T02:25:11ZengMDPI AGMetabolites2218-19892023-04-0113560210.3390/metabo13050602Determination of Indolepropionic Acid and Related Indoles in Plasma, Plasma Ultrafiltrate, and SalivaGeorge M. Anderson0Department of Laboratory Medicine, The Child Study Center, Yale University School of Medicine, 230 S. Frontage Rd., New Haven, CT 06519, USAThe microbial metabolite indolepropionic acid (IPA) and related indolic metabolites, including indolecarboxylic acid (ICA), indolelactic acid (ILA), indoleacetic acid (IAA), indolebutyric acid (IBA), indoxylsulfate (ISO4), and indole, were determined in human plasma, plasma ultrafiltrate (UF), and saliva. The compounds were separated on a 150 × 3 mm column of 3 μm Hypersil C18 eluted with a mobile phase of 80% pH 5 0.01 M sodium acetate containing 1.0 g/L of tert-butylammonium chloride/20% acetonitrile and then detected fluorometrically. Levels of IPA in human plasma UF and of ILA in saliva are reported for the first time. The determination of IPA in plasma UF enables the first report of free plasma IPA, the presumed physiologically active pool of this important microbial metabolite of tryptophan. Plasma and salivary ICA and IBA were not detected, consistent with the absence of any prior reported values. Observed levels or limits of detection for other indolic metabolites usefully supplement limited prior reports.https://www.mdpi.com/2218-1989/13/5/602tryptophanindolepropionic acidindolelactic acidindoleacetic acidindoxylsulfateindole |
| spellingShingle | George M. Anderson Determination of Indolepropionic Acid and Related Indoles in Plasma, Plasma Ultrafiltrate, and Saliva Metabolites tryptophan indolepropionic acid indolelactic acid indoleacetic acid indoxylsulfate indole |
| title | Determination of Indolepropionic Acid and Related Indoles in Plasma, Plasma Ultrafiltrate, and Saliva |
| title_full | Determination of Indolepropionic Acid and Related Indoles in Plasma, Plasma Ultrafiltrate, and Saliva |
| title_fullStr | Determination of Indolepropionic Acid and Related Indoles in Plasma, Plasma Ultrafiltrate, and Saliva |
| title_full_unstemmed | Determination of Indolepropionic Acid and Related Indoles in Plasma, Plasma Ultrafiltrate, and Saliva |
| title_short | Determination of Indolepropionic Acid and Related Indoles in Plasma, Plasma Ultrafiltrate, and Saliva |
| title_sort | determination of indolepropionic acid and related indoles in plasma plasma ultrafiltrate and saliva |
| topic | tryptophan indolepropionic acid indolelactic acid indoleacetic acid indoxylsulfate indole |
| url | https://www.mdpi.com/2218-1989/13/5/602 |
| work_keys_str_mv | AT georgemanderson determinationofindolepropionicacidandrelatedindolesinplasmaplasmaultrafiltrateandsaliva |