Comparative Mitogenomics of Flesh Flies: Implications for Phylogeny
Flesh flies (Diptera: Sarcophagidae) represent a rapid radiation belonging to the Calyptratae. With more than 3000 known species, they are extraordinarily diverse in terms of their breeding habits and are therefore of particular importance in human and veterinary medicine, forensics, and ecology. To...
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MDPI AG
2022-08-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/13/8/718 |
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| author | Jin Shang Wentian Xu Xiaofang Huang Dong Zhang Liping Yan Thomas Pape |
| author_facet | Jin Shang Wentian Xu Xiaofang Huang Dong Zhang Liping Yan Thomas Pape |
| author_sort | Jin Shang |
| collection | DOAJ |
| description | Flesh flies (Diptera: Sarcophagidae) represent a rapid radiation belonging to the Calyptratae. With more than 3000 known species, they are extraordinarily diverse in terms of their breeding habits and are therefore of particular importance in human and veterinary medicine, forensics, and ecology. To better comprehend the phylogenetic relationships and evolutionary characteristics of the Sarcophagidae, we sequenced the complete mitochondrial genomes of five species of flesh flies and performed mitogenomic comparisons amongst the three subfamilies. The mitochondrial genomes match the hypothetical condition of the insect ancestor in terms of gene content and gene arrangement. The evolutionary rates of the subfamilies of Sarcophagidae differ significantly, with Miltogramminae exhibiting a higher rate than the other two subfamilies. The monophyly of the Sarcophagidae and each subfamily is strongly supported by phylogenetic analysis, with the subfamily-level relationship inferred as (Sarcophaginae, (Miltogramminae, Paramacronychiinae)). This study suggests that phylogenetic analysis based on mitochondrial genomes may not be appropriate for rapidly evolving groups such as Miltogramminae and that the third-codon positions could play a considerable role in reconstructing the phylogeny of Sarcophagidae. The protein-coding genes ND2 and ND6 have the potential to be employed as DNA markers for species identification and delimitation in flesh flies. |
| first_indexed | 2024-03-09T13:13:20Z |
| format | Article |
| id | doaj.art-62aaa06477b449e08fe98bb63c9da864 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T13:13:20Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-62aaa06477b449e08fe98bb63c9da8642023-11-30T21:39:10ZengMDPI AGInsects2075-44502022-08-0113871810.3390/insects13080718Comparative Mitogenomics of Flesh Flies: Implications for PhylogenyJin Shang0Wentian Xu1Xiaofang Huang2Dong Zhang3Liping Yan4Thomas Pape5School of Ecology and Nature Conservation, Beijing Forestry University, Beijing 100083, ChinaSchool of Ecology and Nature Conservation, Beijing Forestry University, Beijing 100083, ChinaSchool of Ecology and Nature Conservation, Beijing Forestry University, Beijing 100083, ChinaSchool of Ecology and Nature Conservation, Beijing Forestry University, Beijing 100083, ChinaSchool of Ecology and Nature Conservation, Beijing Forestry University, Beijing 100083, ChinaNatural History Museum of Denmark, University of Copenhagen, Universitetsparken 15, DK-2100 Copenhagen, DenmarkFlesh flies (Diptera: Sarcophagidae) represent a rapid radiation belonging to the Calyptratae. With more than 3000 known species, they are extraordinarily diverse in terms of their breeding habits and are therefore of particular importance in human and veterinary medicine, forensics, and ecology. To better comprehend the phylogenetic relationships and evolutionary characteristics of the Sarcophagidae, we sequenced the complete mitochondrial genomes of five species of flesh flies and performed mitogenomic comparisons amongst the three subfamilies. The mitochondrial genomes match the hypothetical condition of the insect ancestor in terms of gene content and gene arrangement. The evolutionary rates of the subfamilies of Sarcophagidae differ significantly, with Miltogramminae exhibiting a higher rate than the other two subfamilies. The monophyly of the Sarcophagidae and each subfamily is strongly supported by phylogenetic analysis, with the subfamily-level relationship inferred as (Sarcophaginae, (Miltogramminae, Paramacronychiinae)). This study suggests that phylogenetic analysis based on mitochondrial genomes may not be appropriate for rapidly evolving groups such as Miltogramminae and that the third-codon positions could play a considerable role in reconstructing the phylogeny of Sarcophagidae. The protein-coding genes ND2 and ND6 have the potential to be employed as DNA markers for species identification and delimitation in flesh flies.https://www.mdpi.com/2075-4450/13/8/718mitogenomesarcophagidaephylogenyevolution |
| spellingShingle | Jin Shang Wentian Xu Xiaofang Huang Dong Zhang Liping Yan Thomas Pape Comparative Mitogenomics of Flesh Flies: Implications for Phylogeny Insects mitogenome sarcophagidae phylogeny evolution |
| title | Comparative Mitogenomics of Flesh Flies: Implications for Phylogeny |
| title_full | Comparative Mitogenomics of Flesh Flies: Implications for Phylogeny |
| title_fullStr | Comparative Mitogenomics of Flesh Flies: Implications for Phylogeny |
| title_full_unstemmed | Comparative Mitogenomics of Flesh Flies: Implications for Phylogeny |
| title_short | Comparative Mitogenomics of Flesh Flies: Implications for Phylogeny |
| title_sort | comparative mitogenomics of flesh flies implications for phylogeny |
| topic | mitogenome sarcophagidae phylogeny evolution |
| url | https://www.mdpi.com/2075-4450/13/8/718 |
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