Electroactive 3D Printed Scaffolds Based on Percolated Composites of Polycaprolactone with Thermally Reduced Graphene Oxide for Antibacterial and Tissue Engineering Applications
Applying electrical stimulation (ES) could affect different cellular mechanisms, thereby producing a bactericidal effect and an increase in human cell viability. Despite its relevance, this bioelectric effect has been barely reported in percolated conductive biopolymers. In this context, electroacti...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-02-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/3/428 |
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| author | Carolina Angulo-Pineda Kasama Srirussamee Patricia Palma Victor M. Fuenzalida Sarah H. Cartmell Humberto Palza |
| author_facet | Carolina Angulo-Pineda Kasama Srirussamee Patricia Palma Victor M. Fuenzalida Sarah H. Cartmell Humberto Palza |
| author_sort | Carolina Angulo-Pineda |
| collection | DOAJ |
| description | Applying electrical stimulation (ES) could affect different cellular mechanisms, thereby producing a bactericidal effect and an increase in human cell viability. Despite its relevance, this bioelectric effect has been barely reported in percolated conductive biopolymers. In this context, electroactive polycaprolactone (PCL) scaffolds with conductive Thermally Reduced Graphene Oxide (TrGO) nanoparticles were obtained by a 3D printing method. Under direct current (DC) along the percolated scaffolds, a strong antibacterial effect was observed, which completely eradicated <i>S. aureus</i> on the surface of scaffolds. Notably, the same ES regime also produced a four-fold increase in the viability of human mesenchymal stem cells attached to the 3D conductive PCL/TrGO scaffold compared with the pure PCL scaffold. These results have widened the design of novel electroactive composite polymers that could both eliminate the bacteria adhered to the scaffold and increase human cell viability, which have great potential in tissue engineering applications. |
| first_indexed | 2024-04-13T17:01:20Z |
| format | Article |
| id | doaj.art-62ae9db14317424f8ed02398b31836b7 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-04-13T17:01:20Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-62ae9db14317424f8ed02398b31836b72022-12-22T02:38:39ZengMDPI AGNanomaterials2079-49912020-02-0110342810.3390/nano10030428nano10030428Electroactive 3D Printed Scaffolds Based on Percolated Composites of Polycaprolactone with Thermally Reduced Graphene Oxide for Antibacterial and Tissue Engineering ApplicationsCarolina Angulo-Pineda0Kasama Srirussamee1Patricia Palma2Victor M. Fuenzalida3Sarah H. Cartmell4Humberto Palza5Department of Chemical Engineering and Biotechnology and Materials, University of Chile, Santiago 8370456, ChileDepartment of Biomedical Engineering, Faculty of Engineering, King Mongkut’s Institute of Technology Ladkrabang (KMITL), Bangkok 10520, ThailandDepartment of Pathology and Oral Medicine, University of Chile, Santiago 8380492, ChileDepartment of Physics, University of Chile, Santiago 8370449, ChileDepartment of Materials, The University of Manchester, Manchester M13 9PL, UKDepartment of Chemical Engineering and Biotechnology and Materials, University of Chile, Santiago 8370456, ChileApplying electrical stimulation (ES) could affect different cellular mechanisms, thereby producing a bactericidal effect and an increase in human cell viability. Despite its relevance, this bioelectric effect has been barely reported in percolated conductive biopolymers. In this context, electroactive polycaprolactone (PCL) scaffolds with conductive Thermally Reduced Graphene Oxide (TrGO) nanoparticles were obtained by a 3D printing method. Under direct current (DC) along the percolated scaffolds, a strong antibacterial effect was observed, which completely eradicated <i>S. aureus</i> on the surface of scaffolds. Notably, the same ES regime also produced a four-fold increase in the viability of human mesenchymal stem cells attached to the 3D conductive PCL/TrGO scaffold compared with the pure PCL scaffold. These results have widened the design of novel electroactive composite polymers that could both eliminate the bacteria adhered to the scaffold and increase human cell viability, which have great potential in tissue engineering applications.https://www.mdpi.com/2079-4991/10/3/428electroactive biomaterialsconductive polymers3d scaffoldselectrical stimulationbioelectric effectsantibacterial properties |
| spellingShingle | Carolina Angulo-Pineda Kasama Srirussamee Patricia Palma Victor M. Fuenzalida Sarah H. Cartmell Humberto Palza Electroactive 3D Printed Scaffolds Based on Percolated Composites of Polycaprolactone with Thermally Reduced Graphene Oxide for Antibacterial and Tissue Engineering Applications Nanomaterials electroactive biomaterials conductive polymers 3d scaffolds electrical stimulation bioelectric effects antibacterial properties |
| title | Electroactive 3D Printed Scaffolds Based on Percolated Composites of Polycaprolactone with Thermally Reduced Graphene Oxide for Antibacterial and Tissue Engineering Applications |
| title_full | Electroactive 3D Printed Scaffolds Based on Percolated Composites of Polycaprolactone with Thermally Reduced Graphene Oxide for Antibacterial and Tissue Engineering Applications |
| title_fullStr | Electroactive 3D Printed Scaffolds Based on Percolated Composites of Polycaprolactone with Thermally Reduced Graphene Oxide for Antibacterial and Tissue Engineering Applications |
| title_full_unstemmed | Electroactive 3D Printed Scaffolds Based on Percolated Composites of Polycaprolactone with Thermally Reduced Graphene Oxide for Antibacterial and Tissue Engineering Applications |
| title_short | Electroactive 3D Printed Scaffolds Based on Percolated Composites of Polycaprolactone with Thermally Reduced Graphene Oxide for Antibacterial and Tissue Engineering Applications |
| title_sort | electroactive 3d printed scaffolds based on percolated composites of polycaprolactone with thermally reduced graphene oxide for antibacterial and tissue engineering applications |
| topic | electroactive biomaterials conductive polymers 3d scaffolds electrical stimulation bioelectric effects antibacterial properties |
| url | https://www.mdpi.com/2079-4991/10/3/428 |
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