Pemendekan Waktu ELISA dalam Deteksi TMV
In order to support the program for the management of viral diseases on garlic, a rapid diagnosis procedure was developed by shortening the incubation period of the indirect ELISA. No significant differences of ELISA absorbance were observed when the antigen was incubated for 4 h, 2 h, and 30 min at...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada
1996-12-01
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| Series: | Jurnal Perlindungan Tanaman Indonesia |
| Online Access: | https://jurnal.ugm.ac.id/jpti/article/view/12891 |
| _version_ | 1798043205817597952 |
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| author | Susamto Somowiyarjo Y. B. Sumardiyono Purwati Widiyati |
| author_facet | Susamto Somowiyarjo Y. B. Sumardiyono Purwati Widiyati |
| author_sort | Susamto Somowiyarjo |
| collection | DOAJ |
| description | In order to support the program for the management of viral diseases on garlic, a rapid diagnosis procedure was developed by shortening the incubation period of the indirect ELISA. No significant differences of ELISA absorbance were observed when the antigen was incubated for 4 h, 2 h, and 30 min at 37ºC. The incubation period for the antibody and conjugate could he reduced from 4 h at 37ºC or 18 h at 4ºC to 30 min al 37ºC. The shortest period of incubation for the assay could be obtained when each incubation time for the antigen, antibody and conjugate was 30 min. The detection limit for the shortened ELISA was 10^-4 for the virus in crude extracts and 0,5 μg/ml for the purified preparation. |
| first_indexed | 2024-04-11T22:46:11Z |
| format | Article |
| id | doaj.art-62afdc4048fc445ebd924fcbeb98e5df |
| institution | Directory Open Access Journal |
| issn | 1410-1637 2548-4788 |
| language | English |
| last_indexed | 2024-04-11T22:46:11Z |
| publishDate | 1996-12-01 |
| publisher | Universitas Gadjah Mada |
| record_format | Article |
| series | Jurnal Perlindungan Tanaman Indonesia |
| spelling | doaj.art-62afdc4048fc445ebd924fcbeb98e5df2022-12-22T03:58:45ZengUniversitas Gadjah MadaJurnal Perlindungan Tanaman Indonesia1410-16372548-47881996-12-0122667110.22146/jpti.128919744Pemendekan Waktu ELISA dalam Deteksi TMVSusamto Somowiyarjo0Y. B. Sumardiyono1Purwati Widiyati2Fakultas Pertanian Universitas Gadjah MadaFakultas Pertanian Universitas Gadjah MadaFakultas Pertanian Universitas Gadjah MadaIn order to support the program for the management of viral diseases on garlic, a rapid diagnosis procedure was developed by shortening the incubation period of the indirect ELISA. No significant differences of ELISA absorbance were observed when the antigen was incubated for 4 h, 2 h, and 30 min at 37ºC. The incubation period for the antibody and conjugate could he reduced from 4 h at 37ºC or 18 h at 4ºC to 30 min al 37ºC. The shortest period of incubation for the assay could be obtained when each incubation time for the antigen, antibody and conjugate was 30 min. The detection limit for the shortened ELISA was 10^-4 for the virus in crude extracts and 0,5 μg/ml for the purified preparation.https://jurnal.ugm.ac.id/jpti/article/view/12891 |
| spellingShingle | Susamto Somowiyarjo Y. B. Sumardiyono Purwati Widiyati Pemendekan Waktu ELISA dalam Deteksi TMV Jurnal Perlindungan Tanaman Indonesia |
| title | Pemendekan Waktu ELISA dalam Deteksi TMV |
| title_full | Pemendekan Waktu ELISA dalam Deteksi TMV |
| title_fullStr | Pemendekan Waktu ELISA dalam Deteksi TMV |
| title_full_unstemmed | Pemendekan Waktu ELISA dalam Deteksi TMV |
| title_short | Pemendekan Waktu ELISA dalam Deteksi TMV |
| title_sort | pemendekan waktu elisa dalam deteksi tmv |
| url | https://jurnal.ugm.ac.id/jpti/article/view/12891 |
| work_keys_str_mv | AT susamtosomowiyarjo pemendekanwaktuelisadalamdeteksitmv AT ybsumardiyono pemendekanwaktuelisadalamdeteksitmv AT purwatiwidiyati pemendekanwaktuelisadalamdeteksitmv |