Aqueous extract of Sargentodoxa cuneata alleviates ulcerative colitis and its associated liver injuries in mice through the modulation of intestinal flora and related metabolites
BackgroundUlcerative colitis (UC) is a refractory disease worldwide. Liver injury can be found clinically with UC, and now, it is found that gut dysbiosis is an important mechanism in the pathogenesis of UC. Sargentodoxa cuneata has been used as a traditional Chinese medicine and is commonly used cl...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-01-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1295822/full |
| _version_ | 1797347886657175552 |
|---|---|
| author | Feng Xu Piao Yu Hongmei Wu Mei Liu Hongyun Liu Qian Zeng Dengli Wu Xiangpei Wang |
| author_facet | Feng Xu Piao Yu Hongmei Wu Mei Liu Hongyun Liu Qian Zeng Dengli Wu Xiangpei Wang |
| author_sort | Feng Xu |
| collection | DOAJ |
| description | BackgroundUlcerative colitis (UC) is a refractory disease worldwide. Liver injury can be found clinically with UC, and now, it is found that gut dysbiosis is an important mechanism in the pathogenesis of UC. Sargentodoxa cuneata has been used as a traditional Chinese medicine and is commonly used clinically for the treatment of UC. The main objective of this study was to investigate the intrinsic mechanisms of Sargentodoxa cuneata in the treatment of UC and its associated liver injuries from the perspective of intestinal flora and related metabolites.MethodsUltra-performance liquid chromatography-mass spectrometry was used to identify the components in the aqueous extract of Sargentodoxa cuneata (AESc). Mice with UC induced by dextran sulfate sodium were used to study the effects of AESc on UC and its associated liver injuries. Furthermore, 16S rRNA gene sequencing and analysis were performed on intestinal contents, and correlation analysis of intestinal flora with short-chain fatty acids (SCFAs) and organic acids was performed.ResultsA total of 114 compounds were identified in AESc. AESc improved disease activity index scores, liver index, and colon length in mice with UC and had a good protective effect on intestine and liver injuries. Moreover, the administration of AESc regulated gut microbiota dysbiosis and the levels of a few SCFAs and organic acids in mice with UC. In addition, the correlation analysis results showed that the Megamonas and Bifidobacterium were the key intestinal flora related to the levels of differential SCFAs and organic acids in mice with UC after AESc intervention.ConclusionAESc has a good protective effect on UC and UC related liver injuries. Modulation of the intestinal flora and its metabolites (SCFAs and a few organic acids) is an important pathway for AESc in the treatment of UC and also provides a rationale for the clinical use of Sargentodoxa cuneata in the treatment of UC. |
| first_indexed | 2024-03-08T11:54:24Z |
| format | Article |
| id | doaj.art-62b052fdbe4043678d8ebdcb7052bb6c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-08T11:54:24Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-62b052fdbe4043678d8ebdcb7052bb6c2024-01-24T04:46:06ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-01-011510.3389/fmicb.2024.12958221295822Aqueous extract of Sargentodoxa cuneata alleviates ulcerative colitis and its associated liver injuries in mice through the modulation of intestinal flora and related metabolitesFeng Xu0Piao Yu1Hongmei Wu2Mei Liu3Hongyun Liu4Qian Zeng5Dengli Wu6Xiangpei Wang7Department of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang, ChinaDepartment of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang, ChinaDepartment of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang, ChinaDepartment of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang, ChinaDepartment of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang, ChinaDepartment of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang, ChinaDepartment of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang, ChinaSchool of Chinese Ethnic Medicine, Guizhou Minzu University, Guiyang, ChinaBackgroundUlcerative colitis (UC) is a refractory disease worldwide. Liver injury can be found clinically with UC, and now, it is found that gut dysbiosis is an important mechanism in the pathogenesis of UC. Sargentodoxa cuneata has been used as a traditional Chinese medicine and is commonly used clinically for the treatment of UC. The main objective of this study was to investigate the intrinsic mechanisms of Sargentodoxa cuneata in the treatment of UC and its associated liver injuries from the perspective of intestinal flora and related metabolites.MethodsUltra-performance liquid chromatography-mass spectrometry was used to identify the components in the aqueous extract of Sargentodoxa cuneata (AESc). Mice with UC induced by dextran sulfate sodium were used to study the effects of AESc on UC and its associated liver injuries. Furthermore, 16S rRNA gene sequencing and analysis were performed on intestinal contents, and correlation analysis of intestinal flora with short-chain fatty acids (SCFAs) and organic acids was performed.ResultsA total of 114 compounds were identified in AESc. AESc improved disease activity index scores, liver index, and colon length in mice with UC and had a good protective effect on intestine and liver injuries. Moreover, the administration of AESc regulated gut microbiota dysbiosis and the levels of a few SCFAs and organic acids in mice with UC. In addition, the correlation analysis results showed that the Megamonas and Bifidobacterium were the key intestinal flora related to the levels of differential SCFAs and organic acids in mice with UC after AESc intervention.ConclusionAESc has a good protective effect on UC and UC related liver injuries. Modulation of the intestinal flora and its metabolites (SCFAs and a few organic acids) is an important pathway for AESc in the treatment of UC and also provides a rationale for the clinical use of Sargentodoxa cuneata in the treatment of UC.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1295822/fullSargentodoxa cuneataulcerative colitisulcerative colitis-associated liver injuryintestinal florashort-chain fatty acidorganic acid |
| spellingShingle | Feng Xu Piao Yu Hongmei Wu Mei Liu Hongyun Liu Qian Zeng Dengli Wu Xiangpei Wang Aqueous extract of Sargentodoxa cuneata alleviates ulcerative colitis and its associated liver injuries in mice through the modulation of intestinal flora and related metabolites Frontiers in Microbiology Sargentodoxa cuneata ulcerative colitis ulcerative colitis-associated liver injury intestinal flora short-chain fatty acid organic acid |
| title | Aqueous extract of Sargentodoxa cuneata alleviates ulcerative colitis and its associated liver injuries in mice through the modulation of intestinal flora and related metabolites |
| title_full | Aqueous extract of Sargentodoxa cuneata alleviates ulcerative colitis and its associated liver injuries in mice through the modulation of intestinal flora and related metabolites |
| title_fullStr | Aqueous extract of Sargentodoxa cuneata alleviates ulcerative colitis and its associated liver injuries in mice through the modulation of intestinal flora and related metabolites |
| title_full_unstemmed | Aqueous extract of Sargentodoxa cuneata alleviates ulcerative colitis and its associated liver injuries in mice through the modulation of intestinal flora and related metabolites |
| title_short | Aqueous extract of Sargentodoxa cuneata alleviates ulcerative colitis and its associated liver injuries in mice through the modulation of intestinal flora and related metabolites |
| title_sort | aqueous extract of sargentodoxa cuneata alleviates ulcerative colitis and its associated liver injuries in mice through the modulation of intestinal flora and related metabolites |
| topic | Sargentodoxa cuneata ulcerative colitis ulcerative colitis-associated liver injury intestinal flora short-chain fatty acid organic acid |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1295822/full |
| work_keys_str_mv | AT fengxu aqueousextractofsargentodoxacuneataalleviatesulcerativecolitisanditsassociatedliverinjuriesinmicethroughthemodulationofintestinalfloraandrelatedmetabolites AT piaoyu aqueousextractofsargentodoxacuneataalleviatesulcerativecolitisanditsassociatedliverinjuriesinmicethroughthemodulationofintestinalfloraandrelatedmetabolites AT hongmeiwu aqueousextractofsargentodoxacuneataalleviatesulcerativecolitisanditsassociatedliverinjuriesinmicethroughthemodulationofintestinalfloraandrelatedmetabolites AT meiliu aqueousextractofsargentodoxacuneataalleviatesulcerativecolitisanditsassociatedliverinjuriesinmicethroughthemodulationofintestinalfloraandrelatedmetabolites AT hongyunliu aqueousextractofsargentodoxacuneataalleviatesulcerativecolitisanditsassociatedliverinjuriesinmicethroughthemodulationofintestinalfloraandrelatedmetabolites AT qianzeng aqueousextractofsargentodoxacuneataalleviatesulcerativecolitisanditsassociatedliverinjuriesinmicethroughthemodulationofintestinalfloraandrelatedmetabolites AT dengliwu aqueousextractofsargentodoxacuneataalleviatesulcerativecolitisanditsassociatedliverinjuriesinmicethroughthemodulationofintestinalfloraandrelatedmetabolites AT xiangpeiwang aqueousextractofsargentodoxacuneataalleviatesulcerativecolitisanditsassociatedliverinjuriesinmicethroughthemodulationofintestinalfloraandrelatedmetabolites |