GCR1 Positively Regulates UV-B- and Ethylene-Induced Stomatal Closure via Activating GPA1-Dependent ROS and NO Production
Heterotrimeric G proteins function as key players in guard cell signaling to many stimuli, including ultraviolet B (UV-B) and ethylene, but whether guard cell G protein signaling is activated by the only one potential G protein-coupled receptor, GCR1, is still unclear. Here, we found that <i>g...
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2022-05-01
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| author | Xue Li Qi Fu Fu-Xing Zhao Yi-Qing Wu Teng-Yue Zhang Zhong-Qi Li Jun-Min He |
| author_facet | Xue Li Qi Fu Fu-Xing Zhao Yi-Qing Wu Teng-Yue Zhang Zhong-Qi Li Jun-Min He |
| author_sort | Xue Li |
| collection | DOAJ |
| description | Heterotrimeric G proteins function as key players in guard cell signaling to many stimuli, including ultraviolet B (UV-B) and ethylene, but whether guard cell G protein signaling is activated by the only one potential G protein-coupled receptor, GCR1, is still unclear. Here, we found that <i>gcr1</i> null mutants showed defects in UV-B- and ethylene-induced stomatal closure and production of reactive oxygen species (ROS) and nitric oxide (NO) in guard cells, but these defects could be rescued by the application of a Gα activator or overexpression of a constitutively active form of Gα subunit GPA1 (cGPA1). Moreover, the exogenous application of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) or NO triggered stomatal closure in <i>gcr1</i> mutants and <i>cGPA1</i> transgenic plants in the absence or presence of UV-B or ethylene, but exogenous ethylene could not rescue the defect of <i>gcr1</i> mutants in UV-B-induced stomatal closure, and <i>gcr1</i> mutants did not affect UV-B-induced ethylene production in Arabidopsis leaves. These results indicate that GCR1 positively controls UV-B- and ethylene-induced stomatal closure by activating GPA1-dependent ROS and NO production in guard cells and that ethylene acts upstream of GCR1 to transduce UV-B guard cell signaling, which establishes the existence of a classic paradigm of G protein signaling in guard cell signaling to UV-B and ethylene. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:44:25Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-62b416bb7c9c4bcf8e9ba7f0be6138862023-11-23T11:23:56ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012310551210.3390/ijms23105512GCR1 Positively Regulates UV-B- and Ethylene-Induced Stomatal Closure via Activating GPA1-Dependent ROS and NO ProductionXue Li0Qi Fu1Fu-Xing Zhao2Yi-Qing Wu3Teng-Yue Zhang4Zhong-Qi Li5Jun-Min He6School of Life Sciences, Shaanxi Normal University, Xi’an 710119, ChinaSchool of Life Sciences, Shaanxi Normal University, Xi’an 710119, ChinaSchool of Life Sciences, Shaanxi Normal University, Xi’an 710119, ChinaSchool of Life Sciences, Shaanxi Normal University, Xi’an 710119, ChinaSchool of Life Sciences, Shaanxi Normal University, Xi’an 710119, ChinaSchool of Life Sciences, Shaanxi Normal University, Xi’an 710119, ChinaSchool of Life Sciences, Shaanxi Normal University, Xi’an 710119, ChinaHeterotrimeric G proteins function as key players in guard cell signaling to many stimuli, including ultraviolet B (UV-B) and ethylene, but whether guard cell G protein signaling is activated by the only one potential G protein-coupled receptor, GCR1, is still unclear. Here, we found that <i>gcr1</i> null mutants showed defects in UV-B- and ethylene-induced stomatal closure and production of reactive oxygen species (ROS) and nitric oxide (NO) in guard cells, but these defects could be rescued by the application of a Gα activator or overexpression of a constitutively active form of Gα subunit GPA1 (cGPA1). Moreover, the exogenous application of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) or NO triggered stomatal closure in <i>gcr1</i> mutants and <i>cGPA1</i> transgenic plants in the absence or presence of UV-B or ethylene, but exogenous ethylene could not rescue the defect of <i>gcr1</i> mutants in UV-B-induced stomatal closure, and <i>gcr1</i> mutants did not affect UV-B-induced ethylene production in Arabidopsis leaves. These results indicate that GCR1 positively controls UV-B- and ethylene-induced stomatal closure by activating GPA1-dependent ROS and NO production in guard cells and that ethylene acts upstream of GCR1 to transduce UV-B guard cell signaling, which establishes the existence of a classic paradigm of G protein signaling in guard cell signaling to UV-B and ethylene.https://www.mdpi.com/1422-0067/23/10/5512<i>Arabidopsis thaliana</i>ethyleneG protein-coupled receptor GCR1Gα subunit GPA1nitric oxidereactive oxygen species |
| spellingShingle | Xue Li Qi Fu Fu-Xing Zhao Yi-Qing Wu Teng-Yue Zhang Zhong-Qi Li Jun-Min He GCR1 Positively Regulates UV-B- and Ethylene-Induced Stomatal Closure via Activating GPA1-Dependent ROS and NO Production International Journal of Molecular Sciences <i>Arabidopsis thaliana</i> ethylene G protein-coupled receptor GCR1 Gα subunit GPA1 nitric oxide reactive oxygen species |
| title | GCR1 Positively Regulates UV-B- and Ethylene-Induced Stomatal Closure via Activating GPA1-Dependent ROS and NO Production |
| title_full | GCR1 Positively Regulates UV-B- and Ethylene-Induced Stomatal Closure via Activating GPA1-Dependent ROS and NO Production |
| title_fullStr | GCR1 Positively Regulates UV-B- and Ethylene-Induced Stomatal Closure via Activating GPA1-Dependent ROS and NO Production |
| title_full_unstemmed | GCR1 Positively Regulates UV-B- and Ethylene-Induced Stomatal Closure via Activating GPA1-Dependent ROS and NO Production |
| title_short | GCR1 Positively Regulates UV-B- and Ethylene-Induced Stomatal Closure via Activating GPA1-Dependent ROS and NO Production |
| title_sort | gcr1 positively regulates uv b and ethylene induced stomatal closure via activating gpa1 dependent ros and no production |
| topic | <i>Arabidopsis thaliana</i> ethylene G protein-coupled receptor GCR1 Gα subunit GPA1 nitric oxide reactive oxygen species |
| url | https://www.mdpi.com/1422-0067/23/10/5512 |
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