Effective UV/Ozone irradiation method for decontamination of hydroxyapatite surfaces
The purpose of this study was to establish whether UV/ozone (O3) irradiation method can effectively decontaminate hydroxyapatite surfaces, including those modified by the treatment with 30% phosphoric acid solution through morphological and chemical surface analyses (surface roughness, X-ray photoel...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-08-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844017307697 |
| _version_ | 1819145785530384384 |
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| author | Keisuke Yasuda Yohei Okazaki Yasuhiko Abe Kazuhiro Tsuga |
| author_facet | Keisuke Yasuda Yohei Okazaki Yasuhiko Abe Kazuhiro Tsuga |
| author_sort | Keisuke Yasuda |
| collection | DOAJ |
| description | The purpose of this study was to establish whether UV/ozone (O3) irradiation method can effectively decontaminate hydroxyapatite surfaces, including those modified by the treatment with 30% phosphoric acid solution through morphological and chemical surface analyses (surface roughness, X-ray photoelectron spectroscopy and wettability), and to evaluate the in vitro response of osteoblast-like MC3T3-E1 cells to the modified hydroxyapatite surface decontaminated via this method. The amount of carbon and the contact angle of hydroxyapatite surfaces were significantly decreased by UV/O3 irradiation that lasted for ≥ 5 and ≥ 3 min, respectively (P < 0.01). Additionally, 7-day storage of H3PO4-modified hydroxyapatite surface decontaminated with 5-min irradiation did not affect contact angle values (P > 0.05). MC3T3-E1 cell proliferation, differentiation (as assessed by relative ALP and OCN mRNA levels), and mineralisation were significantly promoted on irradiated surfaces (P < 0.05). These findings show that UV/O3 irradiation for ≥ 5 min significantly decontaminated H3PO4-modified hydroxyapatite surface, improved its wettability, and facilitated osteoblast growth and function. |
| first_indexed | 2024-12-22T13:03:33Z |
| format | Article |
| id | doaj.art-62b74029814e4a0c9fd54d3a8f1f4ae3 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-22T13:03:33Z |
| publishDate | 2017-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-62b74029814e4a0c9fd54d3a8f1f4ae32022-12-21T18:24:57ZengElsevierHeliyon2405-84402017-08-013810.1016/j.heliyon.2017.e00372Effective UV/Ozone irradiation method for decontamination of hydroxyapatite surfacesKeisuke YasudaYohei OkazakiYasuhiko AbeKazuhiro TsugaThe purpose of this study was to establish whether UV/ozone (O3) irradiation method can effectively decontaminate hydroxyapatite surfaces, including those modified by the treatment with 30% phosphoric acid solution through morphological and chemical surface analyses (surface roughness, X-ray photoelectron spectroscopy and wettability), and to evaluate the in vitro response of osteoblast-like MC3T3-E1 cells to the modified hydroxyapatite surface decontaminated via this method. The amount of carbon and the contact angle of hydroxyapatite surfaces were significantly decreased by UV/O3 irradiation that lasted for ≥ 5 and ≥ 3 min, respectively (P < 0.01). Additionally, 7-day storage of H3PO4-modified hydroxyapatite surface decontaminated with 5-min irradiation did not affect contact angle values (P > 0.05). MC3T3-E1 cell proliferation, differentiation (as assessed by relative ALP and OCN mRNA levels), and mineralisation were significantly promoted on irradiated surfaces (P < 0.05). These findings show that UV/O3 irradiation for ≥ 5 min significantly decontaminated H3PO4-modified hydroxyapatite surface, improved its wettability, and facilitated osteoblast growth and function.http://www.sciencedirect.com/science/article/pii/S2405844017307697EngineeringBioengineeringMaterials science |
| spellingShingle | Keisuke Yasuda Yohei Okazaki Yasuhiko Abe Kazuhiro Tsuga Effective UV/Ozone irradiation method for decontamination of hydroxyapatite surfaces Heliyon Engineering Bioengineering Materials science |
| title | Effective UV/Ozone irradiation method for decontamination of hydroxyapatite surfaces |
| title_full | Effective UV/Ozone irradiation method for decontamination of hydroxyapatite surfaces |
| title_fullStr | Effective UV/Ozone irradiation method for decontamination of hydroxyapatite surfaces |
| title_full_unstemmed | Effective UV/Ozone irradiation method for decontamination of hydroxyapatite surfaces |
| title_short | Effective UV/Ozone irradiation method for decontamination of hydroxyapatite surfaces |
| title_sort | effective uv ozone irradiation method for decontamination of hydroxyapatite surfaces |
| topic | Engineering Bioengineering Materials science |
| url | http://www.sciencedirect.com/science/article/pii/S2405844017307697 |
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