Simultaneous Screening and Determination of Eleven Mycotoxins in Tomato by Ultra-high Performance Liquid Chromatography-Quadrupole/Orbitrap High-Resolution Mass Spectrometry
A method was developed for the simultaneous screening and determination of eleven mycotoxins in tomato by ultra-high performance liquid chromatography-quadrepole/orbitrap high-resolution mass spectrometry (UPLC-Q/Orbitrap HRMS). Samples were extracted with a mixture of 1% formic acid solution and ac...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
China Food Publishing Company
2023-06-01
|
| Series: | Shipin Kexue |
| Subjects: | |
| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-12-044.pdf |
| _version_ | 1797773143453990912 |
|---|---|
| author | WANG Changzhao, LI Zihao, ZHANG Yali, LIN Fang, LI Jianhua, ZOU Yang, YANG Yue |
| author_facet | WANG Changzhao, LI Zihao, ZHANG Yali, LIN Fang, LI Jianhua, ZOU Yang, YANG Yue |
| author_sort | WANG Changzhao, LI Zihao, ZHANG Yali, LIN Fang, LI Jianhua, ZOU Yang, YANG Yue |
| collection | DOAJ |
| description | A method was developed for the simultaneous screening and determination of eleven mycotoxins in tomato by ultra-high performance liquid chromatography-quadrepole/orbitrap high-resolution mass spectrometry (UPLC-Q/Orbitrap HRMS). Samples were extracted with a mixture of 1% formic acid solution and acetonitrile (99:1, V/V), salted out using a QuEChERS (quick, easy, cheap, effective, rugged and safe) extraction packet, and purified by dispersive solid phase extraction (dSPE). The target compounds were separated on an ACQUITY UPLC BEH shield RP18 column (100 mm × 2.1 mm, 1.7 μm), detected using an electrospray ionization source under positive and negative ion scanning with parallel reaction monitoring. A tandem mass spectral library for rapid screening of the eleven mycotoxins was built based on characteristic fragment ions. The results showed a good linear relationship in the concentration range of 0.25–100.0 mg/L, with correlation coefficients (R2) more than 0.991. The limit of detection (LOD) ranged from 0.1 to 10.0 μg/kg, and the limit of quantification (LOQ) ranged from 0.3 to 30.0 μg/kg. Average recoveries at three spiked levels, LOQ, 2 × LOQ, and 10 × LOQ, were in the range of 78.5%–108.7% with relative standard deviations from 1.0% to 9.3% (n = 6). This method proved to be simple, sensitive, accurate, and suitable for rapid screening and accurate quantification of the eleven mycotoxins in tomato. |
| first_indexed | 2024-03-12T22:02:03Z |
| format | Article |
| id | doaj.art-62b7fabaf0364431834cccbc27039919 |
| institution | Directory Open Access Journal |
| issn | 1002-6630 |
| language | English |
| last_indexed | 2024-03-12T22:02:03Z |
| publishDate | 2023-06-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj.art-62b7fabaf0364431834cccbc270399192023-07-25T06:04:50ZengChina Food Publishing CompanyShipin Kexue1002-66302023-06-01441236837510.7506/spkx1002-6630-20220715-174Simultaneous Screening and Determination of Eleven Mycotoxins in Tomato by Ultra-high Performance Liquid Chromatography-Quadrupole/Orbitrap High-Resolution Mass SpectrometryWANG Changzhao, LI Zihao, ZHANG Yali, LIN Fang, LI Jianhua, ZOU Yang, YANG Yue0(1. Xi’an Customs Technology Center, Xi’an 710068, China; 2. Shaanxi Institute for Food and Drug Control, Xi’an 710065, China;3. Shaanxi Zhongjian Test Technology Co. Ltd., Xi’an 710068, China)A method was developed for the simultaneous screening and determination of eleven mycotoxins in tomato by ultra-high performance liquid chromatography-quadrepole/orbitrap high-resolution mass spectrometry (UPLC-Q/Orbitrap HRMS). Samples were extracted with a mixture of 1% formic acid solution and acetonitrile (99:1, V/V), salted out using a QuEChERS (quick, easy, cheap, effective, rugged and safe) extraction packet, and purified by dispersive solid phase extraction (dSPE). The target compounds were separated on an ACQUITY UPLC BEH shield RP18 column (100 mm × 2.1 mm, 1.7 μm), detected using an electrospray ionization source under positive and negative ion scanning with parallel reaction monitoring. A tandem mass spectral library for rapid screening of the eleven mycotoxins was built based on characteristic fragment ions. The results showed a good linear relationship in the concentration range of 0.25–100.0 mg/L, with correlation coefficients (R2) more than 0.991. The limit of detection (LOD) ranged from 0.1 to 10.0 μg/kg, and the limit of quantification (LOQ) ranged from 0.3 to 30.0 μg/kg. Average recoveries at three spiked levels, LOQ, 2 × LOQ, and 10 × LOQ, were in the range of 78.5%–108.7% with relative standard deviations from 1.0% to 9.3% (n = 6). This method proved to be simple, sensitive, accurate, and suitable for rapid screening and accurate quantification of the eleven mycotoxins in tomato.https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-12-044.pdfultra-high performance liquid chromatography-quadrupole/orbitrap high-resolution mass spectrometry; tomato; quechers; mycotoxins |
| spellingShingle | WANG Changzhao, LI Zihao, ZHANG Yali, LIN Fang, LI Jianhua, ZOU Yang, YANG Yue Simultaneous Screening and Determination of Eleven Mycotoxins in Tomato by Ultra-high Performance Liquid Chromatography-Quadrupole/Orbitrap High-Resolution Mass Spectrometry Shipin Kexue ultra-high performance liquid chromatography-quadrupole/orbitrap high-resolution mass spectrometry; tomato; quechers; mycotoxins |
| title | Simultaneous Screening and Determination of Eleven Mycotoxins in Tomato by Ultra-high Performance Liquid Chromatography-Quadrupole/Orbitrap High-Resolution Mass Spectrometry |
| title_full | Simultaneous Screening and Determination of Eleven Mycotoxins in Tomato by Ultra-high Performance Liquid Chromatography-Quadrupole/Orbitrap High-Resolution Mass Spectrometry |
| title_fullStr | Simultaneous Screening and Determination of Eleven Mycotoxins in Tomato by Ultra-high Performance Liquid Chromatography-Quadrupole/Orbitrap High-Resolution Mass Spectrometry |
| title_full_unstemmed | Simultaneous Screening and Determination of Eleven Mycotoxins in Tomato by Ultra-high Performance Liquid Chromatography-Quadrupole/Orbitrap High-Resolution Mass Spectrometry |
| title_short | Simultaneous Screening and Determination of Eleven Mycotoxins in Tomato by Ultra-high Performance Liquid Chromatography-Quadrupole/Orbitrap High-Resolution Mass Spectrometry |
| title_sort | simultaneous screening and determination of eleven mycotoxins in tomato by ultra high performance liquid chromatography quadrupole orbitrap high resolution mass spectrometry |
| topic | ultra-high performance liquid chromatography-quadrupole/orbitrap high-resolution mass spectrometry; tomato; quechers; mycotoxins |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-12-044.pdf |
| work_keys_str_mv | AT wangchangzhaolizihaozhangyalilinfanglijianhuazouyangyangyue simultaneousscreeninganddeterminationofelevenmycotoxinsintomatobyultrahighperformanceliquidchromatographyquadrupoleorbitraphighresolutionmassspectrometry |