Pancreatic Stellate Cells (PSCs) express Cyclooxygenase-2 (COX-2) and pancreatic cancer stimulates COX-2 in PSCs
<p>Abstract</p> <p>Background</p> <p>Cyclooxygenase 2 (COX-2), the inducible form of prostaglandin G/H synthase, is associated with several human cancers including pancreatic adenocarcinoma. Pancreatic stellate cells (PSCs) play a central role in the intense desmoplasia...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2005-08-01
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| Series: | Molecular Cancer |
| Online Access: | http://www.molecular-cancer.com/content/4/1/27 |
| _version_ | 1811264148499070976 |
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| author | Denham Woody Bell Richard H Talamonti Mark S Pelham Carolyn Ding Xian-Zhong Ujiki Michael Yoshida Seiya Adrian Thomas E |
| author_facet | Denham Woody Bell Richard H Talamonti Mark S Pelham Carolyn Ding Xian-Zhong Ujiki Michael Yoshida Seiya Adrian Thomas E |
| author_sort | Denham Woody |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Cyclooxygenase 2 (COX-2), the inducible form of prostaglandin G/H synthase, is associated with several human cancers including pancreatic adenocarcinoma. Pancreatic stellate cells (PSCs) play a central role in the intense desmoplasia that surrounds pancreatic adenocarcinoma. The present study examined COX-2 expression in PSCs. PSCs isolated from normal rats, were cultured and exposed to conditioned medium (CM) from the human pancreatic cell line, PANC-1.</p> <p>Methods</p> <p>COX-2 expression was evaluated by immunostaining and western blotting. Proliferation of PSCs was determined by thymidine incorporation and cell counting.</p> <p>Results</p> <p>COX-2 was found to be constitutively expressed in PSCs, and COX-2 protein was up-regulated by PANC-1 CM. Moreover, the induction of COX-2 by PANC-1 CM was prevented by U0126, an extracellular signal-regulated kinase (ERK) 1/2 inhibitor suggesting that activation of ERK 1/2 is needed for stimulation of COX-2. Finally, NS398, a selective COX-2 inhibitor, reduced the growth of PSCs by PANC-1 CM, indicating that activation of COX-2 is required for cancer stimulated PSC proliferation.</p> <p>Conclusion</p> <p>The results suggest that COX-2 may play an important role in the regulation of PSC proliferation in response to pancreatic cancer.</p> |
| first_indexed | 2024-04-12T19:58:56Z |
| format | Article |
| id | doaj.art-62bc7d30d7bb47b89d176d3e3720cae4 |
| institution | Directory Open Access Journal |
| issn | 1476-4598 |
| language | English |
| last_indexed | 2024-04-12T19:58:56Z |
| publishDate | 2005-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Cancer |
| spelling | doaj.art-62bc7d30d7bb47b89d176d3e3720cae42022-12-22T03:18:36ZengBMCMolecular Cancer1476-45982005-08-01412710.1186/1476-4598-4-27Pancreatic Stellate Cells (PSCs) express Cyclooxygenase-2 (COX-2) and pancreatic cancer stimulates COX-2 in PSCsDenham WoodyBell Richard HTalamonti Mark SPelham CarolynDing Xian-ZhongUjiki MichaelYoshida SeiyaAdrian Thomas E<p>Abstract</p> <p>Background</p> <p>Cyclooxygenase 2 (COX-2), the inducible form of prostaglandin G/H synthase, is associated with several human cancers including pancreatic adenocarcinoma. Pancreatic stellate cells (PSCs) play a central role in the intense desmoplasia that surrounds pancreatic adenocarcinoma. The present study examined COX-2 expression in PSCs. PSCs isolated from normal rats, were cultured and exposed to conditioned medium (CM) from the human pancreatic cell line, PANC-1.</p> <p>Methods</p> <p>COX-2 expression was evaluated by immunostaining and western blotting. Proliferation of PSCs was determined by thymidine incorporation and cell counting.</p> <p>Results</p> <p>COX-2 was found to be constitutively expressed in PSCs, and COX-2 protein was up-regulated by PANC-1 CM. Moreover, the induction of COX-2 by PANC-1 CM was prevented by U0126, an extracellular signal-regulated kinase (ERK) 1/2 inhibitor suggesting that activation of ERK 1/2 is needed for stimulation of COX-2. Finally, NS398, a selective COX-2 inhibitor, reduced the growth of PSCs by PANC-1 CM, indicating that activation of COX-2 is required for cancer stimulated PSC proliferation.</p> <p>Conclusion</p> <p>The results suggest that COX-2 may play an important role in the regulation of PSC proliferation in response to pancreatic cancer.</p>http://www.molecular-cancer.com/content/4/1/27 |
| spellingShingle | Denham Woody Bell Richard H Talamonti Mark S Pelham Carolyn Ding Xian-Zhong Ujiki Michael Yoshida Seiya Adrian Thomas E Pancreatic Stellate Cells (PSCs) express Cyclooxygenase-2 (COX-2) and pancreatic cancer stimulates COX-2 in PSCs Molecular Cancer |
| title | Pancreatic Stellate Cells (PSCs) express Cyclooxygenase-2 (COX-2) and pancreatic cancer stimulates COX-2 in PSCs |
| title_full | Pancreatic Stellate Cells (PSCs) express Cyclooxygenase-2 (COX-2) and pancreatic cancer stimulates COX-2 in PSCs |
| title_fullStr | Pancreatic Stellate Cells (PSCs) express Cyclooxygenase-2 (COX-2) and pancreatic cancer stimulates COX-2 in PSCs |
| title_full_unstemmed | Pancreatic Stellate Cells (PSCs) express Cyclooxygenase-2 (COX-2) and pancreatic cancer stimulates COX-2 in PSCs |
| title_short | Pancreatic Stellate Cells (PSCs) express Cyclooxygenase-2 (COX-2) and pancreatic cancer stimulates COX-2 in PSCs |
| title_sort | pancreatic stellate cells pscs express cyclooxygenase 2 cox 2 and pancreatic cancer stimulates cox 2 in pscs |
| url | http://www.molecular-cancer.com/content/4/1/27 |
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