Occurrence of Nosemosis in honey bee, Apis mellifera L. at the apiaries of East Kazakhstan
Nosemosis is the most common disease in honey bee Apis mellifera L., and is a major issue related to bee health worldwide. Therefore, the purpose of this research study was to determine prevalence of microsporidia parasitic infection of the genus Nosema spp. in East Kazakhstan Region (EKR). In the y...
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PeerJ Inc.
2022-11-01
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| Online Access: | https://peerj.com/articles/14430.pdf |
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| author | Abdrakhman Baigazanov Yelena Tikhomirova Natalya Valitova Maral Nurkenova Ainur Koigeldinova Elmira Abdullina Olga Zaikovskaya Nurgul Ikimbayeva Dinara Zainettinova Lyailya Bauzhanova |
| author_facet | Abdrakhman Baigazanov Yelena Tikhomirova Natalya Valitova Maral Nurkenova Ainur Koigeldinova Elmira Abdullina Olga Zaikovskaya Nurgul Ikimbayeva Dinara Zainettinova Lyailya Bauzhanova |
| author_sort | Abdrakhman Baigazanov |
| collection | DOAJ |
| description | Nosemosis is the most common disease in honey bee Apis mellifera L., and is a major issue related to bee health worldwide. Therefore, the purpose of this research study was to determine prevalence of microsporidia parasitic infection of the genus Nosema spp. in East Kazakhstan Region (EKR). In the years of 2018 –2021, 394 honey bee samples were collected at 30 apiaries located in four districts of East Kazakhstan Region (Katon-Karagay, Urzhar, Borodulikhinsky, and Shemonaikhinsky). In order to determine the level of infestation, firstly, the presence of Nosema spp. spores was detected using optical microscopy, and then the average amount of spores per bee was counted using a hemocytometer. The degree of nosemosis prevalence was determined in points by means of a semi-quantitative method, and as a percentage from the total of samples and of the amount of positive tests. At the outcome of the study, microsporidia of the genus Nosema spp. were detected in 23.3% of cases (92 samples). Prevalence at its low degree was found in six samples (1.5%), at an average degree in 55 samples (14%), and at a high one in 31 samples (7.9%). This research study proved that microsporidia of the genus Nosema spp. are widely spread at the apiaries of East Kazakhstan Region in different orographic and climatic conditions. Notwithstanding that it was impossible to statistically determine any significant differences between the dependence of nosemosis prevalence and the apiary location, this indicator is actually higher in the mountainous regions than in the steppe. Concurrently, a close inverse correlation was recognized between the amount of spores in one bee and the level of infestation in bee families from the duration of the vegetation season at the apiary location. This gives grounds to assert that the environmental factors have an impact on formation and development of nosemosis. The results of the research presented in the article indicate the need for further research aimed at increasing the number of studied apiaries, and above all the use of molecular biology methods to distinguish the species that cause nosemosis infection (PCR). |
| first_indexed | 2024-03-09T08:18:33Z |
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| id | doaj.art-62c2a1cafccd4fd0acf1ed0a518521d3 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T08:18:33Z |
| publishDate | 2022-11-01 |
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| spelling | doaj.art-62c2a1cafccd4fd0acf1ed0a518521d32023-12-02T21:55:20ZengPeerJ Inc.PeerJ2167-83592022-11-0110e1443010.7717/peerj.14430Occurrence of Nosemosis in honey bee, Apis mellifera L. at the apiaries of East KazakhstanAbdrakhman Baigazanov0Yelena Tikhomirova1Natalya Valitova2Maral Nurkenova3Ainur Koigeldinova4Elmira Abdullina5Olga Zaikovskaya6Nurgul Ikimbayeva7Dinara Zainettinova8Lyailya Bauzhanova9Department of Veterinary, Faculty of Veterinary Medicine and Agricultural Management, Shakarim University, Semey, East Kazakhstan Region, KazakhstanDepartment of Veterinary, Faculty of Veterinary Medicine and Agricultural Management, Shakarim University, Semey, East Kazakhstan Region, KazakhstanSchool of Earth and Environmental Sciences, D. Serikbayev East Kazakhstan Technical University, Ust-Kamenogorsk, East Kazakhstan Region, KazakhstanDepartment of Veterinary, Faculty of Veterinary Medicine and Agricultural Management, Shakarim University, Semey, East Kazakhstan Region, KazakhstanAgrotechnopark Scientific Center, Veterinary and Food Safety Laboratory, Shakarim University, Semey, East Kazakstan Region, KazakhstanDepartment of Veterinary, Faculty of Veterinary Medicine and Agricultural Management, Shakarim University, Semey, East Kazakhstan Region, KazakhstanAgrotechnopark Scientific Center, Veterinary and Food Safety Laboratory, Shakarim University, Semey, East Kazakstan Region, KazakhstanAgrotechnopark Scientific Center, Veterinary and Food Safety Laboratory, Shakarim University, Semey, East Kazakstan Region, KazakhstanAgrotechnopark Scientific Center, Veterinary and Food Safety Laboratory, Shakarim University, Semey, East Kazakstan Region, KazakhstanDepartment of Zoo Technology, Genetics and Breeding, Toraighyrov University, Pavlodar, Pavlodar Region, KazakhstanNosemosis is the most common disease in honey bee Apis mellifera L., and is a major issue related to bee health worldwide. Therefore, the purpose of this research study was to determine prevalence of microsporidia parasitic infection of the genus Nosema spp. in East Kazakhstan Region (EKR). In the years of 2018 –2021, 394 honey bee samples were collected at 30 apiaries located in four districts of East Kazakhstan Region (Katon-Karagay, Urzhar, Borodulikhinsky, and Shemonaikhinsky). In order to determine the level of infestation, firstly, the presence of Nosema spp. spores was detected using optical microscopy, and then the average amount of spores per bee was counted using a hemocytometer. The degree of nosemosis prevalence was determined in points by means of a semi-quantitative method, and as a percentage from the total of samples and of the amount of positive tests. At the outcome of the study, microsporidia of the genus Nosema spp. were detected in 23.3% of cases (92 samples). Prevalence at its low degree was found in six samples (1.5%), at an average degree in 55 samples (14%), and at a high one in 31 samples (7.9%). This research study proved that microsporidia of the genus Nosema spp. are widely spread at the apiaries of East Kazakhstan Region in different orographic and climatic conditions. Notwithstanding that it was impossible to statistically determine any significant differences between the dependence of nosemosis prevalence and the apiary location, this indicator is actually higher in the mountainous regions than in the steppe. Concurrently, a close inverse correlation was recognized between the amount of spores in one bee and the level of infestation in bee families from the duration of the vegetation season at the apiary location. This gives grounds to assert that the environmental factors have an impact on formation and development of nosemosis. The results of the research presented in the article indicate the need for further research aimed at increasing the number of studied apiaries, and above all the use of molecular biology methods to distinguish the species that cause nosemosis infection (PCR).https://peerj.com/articles/14430.pdfHoney beeApis mellifera L.MicrosporidiaNosemosisMicroscopic countingEast Kazakhstan Region |
| spellingShingle | Abdrakhman Baigazanov Yelena Tikhomirova Natalya Valitova Maral Nurkenova Ainur Koigeldinova Elmira Abdullina Olga Zaikovskaya Nurgul Ikimbayeva Dinara Zainettinova Lyailya Bauzhanova Occurrence of Nosemosis in honey bee, Apis mellifera L. at the apiaries of East Kazakhstan PeerJ Honey bee Apis mellifera L. Microsporidia Nosemosis Microscopic counting East Kazakhstan Region |
| title | Occurrence of Nosemosis in honey bee, Apis mellifera L. at the apiaries of East Kazakhstan |
| title_full | Occurrence of Nosemosis in honey bee, Apis mellifera L. at the apiaries of East Kazakhstan |
| title_fullStr | Occurrence of Nosemosis in honey bee, Apis mellifera L. at the apiaries of East Kazakhstan |
| title_full_unstemmed | Occurrence of Nosemosis in honey bee, Apis mellifera L. at the apiaries of East Kazakhstan |
| title_short | Occurrence of Nosemosis in honey bee, Apis mellifera L. at the apiaries of East Kazakhstan |
| title_sort | occurrence of nosemosis in honey bee apis mellifera l at the apiaries of east kazakhstan |
| topic | Honey bee Apis mellifera L. Microsporidia Nosemosis Microscopic counting East Kazakhstan Region |
| url | https://peerj.com/articles/14430.pdf |
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