Beneficial Effects of Walnut Oligopeptides on Muscle Loss in Senescence-Accelerated Mouse Prone-8 (SAMP8) Mice: Focusing on Mitochondrial Function
Aging-related muscle loss is a hallmark of aging and is the cause of some negative outcomes. An optimized diet and supplements have a positive effect in slowing down the process of muscle loss. This study was designed to evaluate the beneficial effects of walnut oligopeptides (WOPs) on aging-related...
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MDPI AG
2022-05-01
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| Series: | Nutrients |
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| author | Rui Fan Yuntao Hao Qian Du Jiawei Kang Meihong Xu Yong Li |
| author_facet | Rui Fan Yuntao Hao Qian Du Jiawei Kang Meihong Xu Yong Li |
| author_sort | Rui Fan |
| collection | DOAJ |
| description | Aging-related muscle loss is a hallmark of aging and is the cause of some negative outcomes. An optimized diet and supplements have a positive effect in slowing down the process of muscle loss. This study was designed to evaluate the beneficial effects of walnut oligopeptides (WOPs) on aging-related muscle loss and explore the possible underlying mechanism in Senescence-Accelerated Mouse Prone 8 (SAMP8) Mice. SAMP8 mice were randomly divided into four groups (<i>n</i> = 15/group), including one group which was the SAMP8 age control group and three groups those were WOP intervention groups. Meanwhile, Senescence Accelerated Resistant Mouse 1 (SAMR1) mice (<i>n</i> = 12), which had normal senescence rates, were used as model controls. During the six-month intervention period, the age control and normal control groups were given sterilized water, while the three WOP intervention groups were given WOP solution with low (110 mg/kg·bw), medium (220 mg/kg·bw) and high concentrations (440 mg/kg·bw), respectively. The results showed that WOPs could significantly increase muscle mass and improve physical performance (wire hang and catwalk behavioral tests) in aging mice. Moreover, WOPs could significantly reduce the levels of IL-1β, IL-6 and TNF-α in serum and gastrocnemius tissues and increase the mitochondrial DNA content, as well as the expression levels of AMPK, PGC-1α, NRF-1 and TFAM in the gastrocnemius muscle of aging mice, which was speculated to be the specific mechanism related to mitochondrial function improvement and inflammation reduction. These results indicate that WOPs can improve aging-related muscle loss, in term of both muscle mass and physical performance, and WOP supplements seems to be potentially effective in elderly individuals. |
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| series | Nutrients |
| spelling | doaj.art-62c440afbc6d4e6cab0f10be2da0219b2023-11-23T12:29:14ZengMDPI AGNutrients2072-66432022-05-011410205110.3390/nu14102051Beneficial Effects of Walnut Oligopeptides on Muscle Loss in Senescence-Accelerated Mouse Prone-8 (SAMP8) Mice: Focusing on Mitochondrial FunctionRui Fan0Yuntao Hao1Qian Du2Jiawei Kang3Meihong Xu4Yong Li5Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, ChinaDepartment of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, ChinaDepartment of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, ChinaDepartment of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, ChinaDepartment of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, ChinaDepartment of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, ChinaAging-related muscle loss is a hallmark of aging and is the cause of some negative outcomes. An optimized diet and supplements have a positive effect in slowing down the process of muscle loss. This study was designed to evaluate the beneficial effects of walnut oligopeptides (WOPs) on aging-related muscle loss and explore the possible underlying mechanism in Senescence-Accelerated Mouse Prone 8 (SAMP8) Mice. SAMP8 mice were randomly divided into four groups (<i>n</i> = 15/group), including one group which was the SAMP8 age control group and three groups those were WOP intervention groups. Meanwhile, Senescence Accelerated Resistant Mouse 1 (SAMR1) mice (<i>n</i> = 12), which had normal senescence rates, were used as model controls. During the six-month intervention period, the age control and normal control groups were given sterilized water, while the three WOP intervention groups were given WOP solution with low (110 mg/kg·bw), medium (220 mg/kg·bw) and high concentrations (440 mg/kg·bw), respectively. The results showed that WOPs could significantly increase muscle mass and improve physical performance (wire hang and catwalk behavioral tests) in aging mice. Moreover, WOPs could significantly reduce the levels of IL-1β, IL-6 and TNF-α in serum and gastrocnemius tissues and increase the mitochondrial DNA content, as well as the expression levels of AMPK, PGC-1α, NRF-1 and TFAM in the gastrocnemius muscle of aging mice, which was speculated to be the specific mechanism related to mitochondrial function improvement and inflammation reduction. These results indicate that WOPs can improve aging-related muscle loss, in term of both muscle mass and physical performance, and WOP supplements seems to be potentially effective in elderly individuals.https://www.mdpi.com/2072-6643/14/10/2051walnut oligopeptidesSAMP8mitochondrial functionmuscle lossanti-inflammatory |
| spellingShingle | Rui Fan Yuntao Hao Qian Du Jiawei Kang Meihong Xu Yong Li Beneficial Effects of Walnut Oligopeptides on Muscle Loss in Senescence-Accelerated Mouse Prone-8 (SAMP8) Mice: Focusing on Mitochondrial Function Nutrients walnut oligopeptides SAMP8 mitochondrial function muscle loss anti-inflammatory |
| title | Beneficial Effects of Walnut Oligopeptides on Muscle Loss in Senescence-Accelerated Mouse Prone-8 (SAMP8) Mice: Focusing on Mitochondrial Function |
| title_full | Beneficial Effects of Walnut Oligopeptides on Muscle Loss in Senescence-Accelerated Mouse Prone-8 (SAMP8) Mice: Focusing on Mitochondrial Function |
| title_fullStr | Beneficial Effects of Walnut Oligopeptides on Muscle Loss in Senescence-Accelerated Mouse Prone-8 (SAMP8) Mice: Focusing on Mitochondrial Function |
| title_full_unstemmed | Beneficial Effects of Walnut Oligopeptides on Muscle Loss in Senescence-Accelerated Mouse Prone-8 (SAMP8) Mice: Focusing on Mitochondrial Function |
| title_short | Beneficial Effects of Walnut Oligopeptides on Muscle Loss in Senescence-Accelerated Mouse Prone-8 (SAMP8) Mice: Focusing on Mitochondrial Function |
| title_sort | beneficial effects of walnut oligopeptides on muscle loss in senescence accelerated mouse prone 8 samp8 mice focusing on mitochondrial function |
| topic | walnut oligopeptides SAMP8 mitochondrial function muscle loss anti-inflammatory |
| url | https://www.mdpi.com/2072-6643/14/10/2051 |
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