Analysis of the bovine monocyte-derived macrophage response to Mycobacterium avium subspecies paratuberculosis infection using RNA-seq
Johne’s disease, caused by infection with Mycobacterium avium subsp. paratuberculosis, (MAP), is a chronic intestinal disease of ruminants with serious economic consequences for cattle production in the United States and elsewhere. During infection, MAP bacilli are phagocytosed and subvert host macr...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-02-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00023/full |
| _version_ | 1819024956549234688 |
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| author | Maura E Casey Maura E Casey Kieran G. Meade Nicolas C Nalpas Maria eTaraktsoglou John A Browne Kate E Killick Kate E Killick Stephen David Edward Park Stephen David Edward Park Eamonn eGormley Karsten eHokamp David A Magee David A Magee David E Machugh David E Machugh |
| author_facet | Maura E Casey Maura E Casey Kieran G. Meade Nicolas C Nalpas Maria eTaraktsoglou John A Browne Kate E Killick Kate E Killick Stephen David Edward Park Stephen David Edward Park Eamonn eGormley Karsten eHokamp David A Magee David A Magee David E Machugh David E Machugh |
| author_sort | Maura E Casey |
| collection | DOAJ |
| description | Johne’s disease, caused by infection with Mycobacterium avium subsp. paratuberculosis, (MAP), is a chronic intestinal disease of ruminants with serious economic consequences for cattle production in the United States and elsewhere. During infection, MAP bacilli are phagocytosed and subvert host macrophage processes, resulting in subclinical infections that can lead to immunopathology and dissemination of disease. Analysis of the host macrophage transcriptome during infection can therefore shed light on the molecular mechanisms and host-pathogen interplay associated with Johne’s disease. Here we describe results of an in vitro study of the bovine monocyte-derived macrophage (MDM) transcriptome response during MAP infection using RNA-seq. MDM were obtained from seven age- and sex-matched Holstein-Friesian cattle and were infected with MAP across a six-hour infection time course with non-infected controls. We observed 245 and 574 differentially expressed genes in MAP-infected versus non-infected control samples (adjusted P value ≤ 0.05) at 2 and 6 hours post-infection, respectively. Functional analyses of these differentially expressed genes, including biological pathway enrichment, highlighted potential functional roles for genes that have not been previously described in the host response to infection with MAP bacilli. In addition, differential expression of pro- and anti-inflammatory cytokine genes, such as those associated with the IL-10 signaling pathway, and other immune-related genes that encode proteins involved in the bovine macrophage response to MAP infection emphasize the balance between protective host immunity and bacilli survival and proliferation. Systematic comparisons of RNA-seq gene expression results with Affymetrix® microarray data generated from the same experimental samples also demonstrated that RNA-seq represents a superior technology for studying host transcriptional responses to intracellular infection. |
| first_indexed | 2024-12-21T05:03:01Z |
| format | Article |
| id | doaj.art-62c531bc224b41648374f05227387955 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-21T05:03:01Z |
| publishDate | 2015-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-62c531bc224b41648374f052273879552022-12-21T19:15:12ZengFrontiers Media S.A.Frontiers in Immunology1664-32242015-02-01610.3389/fimmu.2015.00023118960Analysis of the bovine monocyte-derived macrophage response to Mycobacterium avium subspecies paratuberculosis infection using RNA-seqMaura E Casey0Maura E Casey1Kieran G. Meade2Nicolas C Nalpas3Maria eTaraktsoglou4John A Browne5Kate E Killick6Kate E Killick7Stephen David Edward Park8Stephen David Edward Park9Eamonn eGormley10Karsten eHokamp11David A Magee12David A Magee13David E Machugh14David E Machugh15University College DublinTeagascTeagascUniversity College DublinHealth and Safety ExecutiveUniversity College DublinUniversity College DublinUniversity College DublinUniversity College DublinIdentiGEN Ltd.University College DublinTrinity College DublinUniversity College DublinUniversity of ConnecticutUniversity College DublinUniversity College DublinJohne’s disease, caused by infection with Mycobacterium avium subsp. paratuberculosis, (MAP), is a chronic intestinal disease of ruminants with serious economic consequences for cattle production in the United States and elsewhere. During infection, MAP bacilli are phagocytosed and subvert host macrophage processes, resulting in subclinical infections that can lead to immunopathology and dissemination of disease. Analysis of the host macrophage transcriptome during infection can therefore shed light on the molecular mechanisms and host-pathogen interplay associated with Johne’s disease. Here we describe results of an in vitro study of the bovine monocyte-derived macrophage (MDM) transcriptome response during MAP infection using RNA-seq. MDM were obtained from seven age- and sex-matched Holstein-Friesian cattle and were infected with MAP across a six-hour infection time course with non-infected controls. We observed 245 and 574 differentially expressed genes in MAP-infected versus non-infected control samples (adjusted P value ≤ 0.05) at 2 and 6 hours post-infection, respectively. Functional analyses of these differentially expressed genes, including biological pathway enrichment, highlighted potential functional roles for genes that have not been previously described in the host response to infection with MAP bacilli. In addition, differential expression of pro- and anti-inflammatory cytokine genes, such as those associated with the IL-10 signaling pathway, and other immune-related genes that encode proteins involved in the bovine macrophage response to MAP infection emphasize the balance between protective host immunity and bacilli survival and proliferation. Systematic comparisons of RNA-seq gene expression results with Affymetrix® microarray data generated from the same experimental samples also demonstrated that RNA-seq represents a superior technology for studying host transcriptional responses to intracellular infection.http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00023/fullCattleTranscriptomeMicroarrayimmune responsemacrophageJohne’s disease |
| spellingShingle | Maura E Casey Maura E Casey Kieran G. Meade Nicolas C Nalpas Maria eTaraktsoglou John A Browne Kate E Killick Kate E Killick Stephen David Edward Park Stephen David Edward Park Eamonn eGormley Karsten eHokamp David A Magee David A Magee David E Machugh David E Machugh Analysis of the bovine monocyte-derived macrophage response to Mycobacterium avium subspecies paratuberculosis infection using RNA-seq Frontiers in Immunology Cattle Transcriptome Microarray immune response macrophage Johne’s disease |
| title | Analysis of the bovine monocyte-derived macrophage response to Mycobacterium avium subspecies paratuberculosis infection using RNA-seq |
| title_full | Analysis of the bovine monocyte-derived macrophage response to Mycobacterium avium subspecies paratuberculosis infection using RNA-seq |
| title_fullStr | Analysis of the bovine monocyte-derived macrophage response to Mycobacterium avium subspecies paratuberculosis infection using RNA-seq |
| title_full_unstemmed | Analysis of the bovine monocyte-derived macrophage response to Mycobacterium avium subspecies paratuberculosis infection using RNA-seq |
| title_short | Analysis of the bovine monocyte-derived macrophage response to Mycobacterium avium subspecies paratuberculosis infection using RNA-seq |
| title_sort | analysis of the bovine monocyte derived macrophage response to mycobacterium avium subspecies paratuberculosis infection using rna seq |
| topic | Cattle Transcriptome Microarray immune response macrophage Johne’s disease |
| url | http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00023/full |
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