Signaling Through Purinergic Receptor P2Y<sub>2</sub> Enhances Macrophage IL-1β Production
The release of nucleotides during necrosis or apoptosis has been described to have both proinflammatory and anti-inflammatory effect on the surrounding cells. Here we describe how low concentrations of UTP and ATP applied during macrophage priming enhance IL-1β production when subsequently the NLRP3...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/13/4686 |
| _version_ | 1797563646912495616 |
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| author | Gonzalo de la Rosa Ana I. Gómez María C. Baños Pablo Pelegrín |
| author_facet | Gonzalo de la Rosa Ana I. Gómez María C. Baños Pablo Pelegrín |
| author_sort | Gonzalo de la Rosa |
| collection | DOAJ |
| description | The release of nucleotides during necrosis or apoptosis has been described to have both proinflammatory and anti-inflammatory effect on the surrounding cells. Here we describe how low concentrations of UTP and ATP applied during macrophage priming enhance IL-1β production when subsequently the NLRP3 inflammasome is activated in murine resident peritoneal macrophages. Deficiency or pharmacological inhibition of the purinergic receptor P2Y<sub>2</sub> reverted the increase of IL-1β release induced by nucleotides. IL-1β increase was found dependent on the expression of <i>Il1b</i> gene and probably involving JNK activity. On the contrary, nucleotides decreased the production of a different proinflammatory cytokines such as TNF-α. These results suggest that nucleotides could shape the response of macrophages to obtain a unique proinflammatory signature that might be relevant in unrevealing specific inflammatory conditions. |
| first_indexed | 2024-03-10T18:46:25Z |
| format | Article |
| id | doaj.art-62c6ac1cc2ae4126b4ab425fe39d98b2 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T18:46:25Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-62c6ac1cc2ae4126b4ab425fe39d98b22023-11-20T05:30:16ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-06-012113468610.3390/ijms21134686Signaling Through Purinergic Receptor P2Y<sub>2</sub> Enhances Macrophage IL-1β ProductionGonzalo de la Rosa0Ana I. Gómez1María C. Baños2Pablo Pelegrín3Unidad de Inflamación Molecular y Cirugía Experimental, Instituto Murciano de Investigación Biosanitaria IMIB-Arrixaca, Hospital Clínico Universitario Virgen de la Arrixaca, 30120 Murcia, SpainUnidad de Inflamación Molecular y Cirugía Experimental, Instituto Murciano de Investigación Biosanitaria IMIB-Arrixaca, Hospital Clínico Universitario Virgen de la Arrixaca, 30120 Murcia, SpainUnidad de Inflamación Molecular y Cirugía Experimental, Instituto Murciano de Investigación Biosanitaria IMIB-Arrixaca, Hospital Clínico Universitario Virgen de la Arrixaca, 30120 Murcia, SpainUnidad de Inflamación Molecular y Cirugía Experimental, Instituto Murciano de Investigación Biosanitaria IMIB-Arrixaca, Hospital Clínico Universitario Virgen de la Arrixaca, 30120 Murcia, SpainThe release of nucleotides during necrosis or apoptosis has been described to have both proinflammatory and anti-inflammatory effect on the surrounding cells. Here we describe how low concentrations of UTP and ATP applied during macrophage priming enhance IL-1β production when subsequently the NLRP3 inflammasome is activated in murine resident peritoneal macrophages. Deficiency or pharmacological inhibition of the purinergic receptor P2Y<sub>2</sub> reverted the increase of IL-1β release induced by nucleotides. IL-1β increase was found dependent on the expression of <i>Il1b</i> gene and probably involving JNK activity. On the contrary, nucleotides decreased the production of a different proinflammatory cytokines such as TNF-α. These results suggest that nucleotides could shape the response of macrophages to obtain a unique proinflammatory signature that might be relevant in unrevealing specific inflammatory conditions.https://www.mdpi.com/1422-0067/21/13/4686purinergic signalingmacrophagesinflammationcytokinesextracellular nucleotides |
| spellingShingle | Gonzalo de la Rosa Ana I. Gómez María C. Baños Pablo Pelegrín Signaling Through Purinergic Receptor P2Y<sub>2</sub> Enhances Macrophage IL-1β Production International Journal of Molecular Sciences purinergic signaling macrophages inflammation cytokines extracellular nucleotides |
| title | Signaling Through Purinergic Receptor P2Y<sub>2</sub> Enhances Macrophage IL-1β Production |
| title_full | Signaling Through Purinergic Receptor P2Y<sub>2</sub> Enhances Macrophage IL-1β Production |
| title_fullStr | Signaling Through Purinergic Receptor P2Y<sub>2</sub> Enhances Macrophage IL-1β Production |
| title_full_unstemmed | Signaling Through Purinergic Receptor P2Y<sub>2</sub> Enhances Macrophage IL-1β Production |
| title_short | Signaling Through Purinergic Receptor P2Y<sub>2</sub> Enhances Macrophage IL-1β Production |
| title_sort | signaling through purinergic receptor p2y sub 2 sub enhances macrophage il 1β production |
| topic | purinergic signaling macrophages inflammation cytokines extracellular nucleotides |
| url | https://www.mdpi.com/1422-0067/21/13/4686 |
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