Rcf1 Modulates Cytochrome c Oxidase Activity Especially Under Energy-Demanding Conditions
The mitochondrial respiratory chain is assembled into supercomplexes. Previously, two respiratory supercomplex-associated proteins, Rcf1 and Rcf2, were identified in Saccharomyces cerevisiae, which were initially suggested to mediate supercomplex formation. Recent evidence suggests that these factor...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-01-01
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| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2019.01555/full |
| _version_ | 1828473893091803136 |
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| author | Hannah Dawitz Jacob Schäfer Judith M. Schaart Wout Magits Peter Brzezinski Martin Ott |
| author_facet | Hannah Dawitz Jacob Schäfer Judith M. Schaart Wout Magits Peter Brzezinski Martin Ott |
| author_sort | Hannah Dawitz |
| collection | DOAJ |
| description | The mitochondrial respiratory chain is assembled into supercomplexes. Previously, two respiratory supercomplex-associated proteins, Rcf1 and Rcf2, were identified in Saccharomyces cerevisiae, which were initially suggested to mediate supercomplex formation. Recent evidence suggests that these factors instead are involved in cytochrome c oxidase biogenesis. We demonstrate here that Rcf1 mediates proper function of cytochrome c oxidase, while binding of Rcf2 results in a decrease of cytochrome c oxidase activity. Chemical crosslink experiments demonstrate that the conserved Hig-domain as well as the fungi specific C-terminus of Rcf1 are involved in molecular interactions with the cytochrome c oxidase subunit Cox3. We propose that Rcf1 modulates cytochrome c oxidase activity by direct binding to the oxidase to trigger changes in subunit Cox1, which harbors the catalytic site. Additionally, Rcf1 interaction with cytochrome c oxidase in the supercomplexes increases under respiratory conditions. These observations indicate that Rcf1 could enable the tuning of the respiratory chain depending on metabolic needs or repair damages at the catalytic site. |
| first_indexed | 2024-12-11T05:51:27Z |
| format | Article |
| id | doaj.art-62c6c06f1d8b45cda2f5113bcd02d94a |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-11T05:51:27Z |
| publishDate | 2020-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-62c6c06f1d8b45cda2f5113bcd02d94a2022-12-22T01:18:48ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2020-01-011010.3389/fphys.2019.01555503994Rcf1 Modulates Cytochrome c Oxidase Activity Especially Under Energy-Demanding ConditionsHannah DawitzJacob SchäferJudith M. SchaartWout MagitsPeter BrzezinskiMartin OttThe mitochondrial respiratory chain is assembled into supercomplexes. Previously, two respiratory supercomplex-associated proteins, Rcf1 and Rcf2, were identified in Saccharomyces cerevisiae, which were initially suggested to mediate supercomplex formation. Recent evidence suggests that these factors instead are involved in cytochrome c oxidase biogenesis. We demonstrate here that Rcf1 mediates proper function of cytochrome c oxidase, while binding of Rcf2 results in a decrease of cytochrome c oxidase activity. Chemical crosslink experiments demonstrate that the conserved Hig-domain as well as the fungi specific C-terminus of Rcf1 are involved in molecular interactions with the cytochrome c oxidase subunit Cox3. We propose that Rcf1 modulates cytochrome c oxidase activity by direct binding to the oxidase to trigger changes in subunit Cox1, which harbors the catalytic site. Additionally, Rcf1 interaction with cytochrome c oxidase in the supercomplexes increases under respiratory conditions. These observations indicate that Rcf1 could enable the tuning of the respiratory chain depending on metabolic needs or repair damages at the catalytic site.https://www.frontiersin.org/article/10.3389/fphys.2019.01555/fullRcf1Rcf2respiratory supercomplexcytochrome c oxidasebc1 complexinteraction partners |
| spellingShingle | Hannah Dawitz Jacob Schäfer Judith M. Schaart Wout Magits Peter Brzezinski Martin Ott Rcf1 Modulates Cytochrome c Oxidase Activity Especially Under Energy-Demanding Conditions Frontiers in Physiology Rcf1 Rcf2 respiratory supercomplex cytochrome c oxidase bc1 complex interaction partners |
| title | Rcf1 Modulates Cytochrome c Oxidase Activity Especially Under Energy-Demanding Conditions |
| title_full | Rcf1 Modulates Cytochrome c Oxidase Activity Especially Under Energy-Demanding Conditions |
| title_fullStr | Rcf1 Modulates Cytochrome c Oxidase Activity Especially Under Energy-Demanding Conditions |
| title_full_unstemmed | Rcf1 Modulates Cytochrome c Oxidase Activity Especially Under Energy-Demanding Conditions |
| title_short | Rcf1 Modulates Cytochrome c Oxidase Activity Especially Under Energy-Demanding Conditions |
| title_sort | rcf1 modulates cytochrome c oxidase activity especially under energy demanding conditions |
| topic | Rcf1 Rcf2 respiratory supercomplex cytochrome c oxidase bc1 complex interaction partners |
| url | https://www.frontiersin.org/article/10.3389/fphys.2019.01555/full |
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