Comprehensive transcriptome analysis and functional characterization of PR-5 for its involvement in tomato Sw-7 resistance to tomato spotted wilt tospovirus
Abstract Tomato spotted wilt tospovirus (TSWV), one of the most important plant viruses, causes yield losses to many crops including tomato. The current disease management for TSWV is based mainly on breeding tomato cultivars containing the Sw-5 locus. Unfortunately, several Sw-5 resistance-breaking...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2019-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-019-44100-x |
| _version_ | 1830416799229804544 |
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| author | Chellappan Padmanabhan Qiyue Ma Reza Shekasteband Kevin S. Stewart Samuel F. Hutton John W. Scott Zhangjun Fei Kai-Shu Ling |
| author_facet | Chellappan Padmanabhan Qiyue Ma Reza Shekasteband Kevin S. Stewart Samuel F. Hutton John W. Scott Zhangjun Fei Kai-Shu Ling |
| author_sort | Chellappan Padmanabhan |
| collection | DOAJ |
| description | Abstract Tomato spotted wilt tospovirus (TSWV), one of the most important plant viruses, causes yield losses to many crops including tomato. The current disease management for TSWV is based mainly on breeding tomato cultivars containing the Sw-5 locus. Unfortunately, several Sw-5 resistance-breaking strains of TSWV have been identified. Sw-7 is an alternative locus conferring resistance to a broad range of TSWV strains. In an effort to uncover gene networks that are associated with the Sw-7 resistance, we performed a comparative transcriptome profiling and gene expression analysis between a nearly-isogenic Sw-7 line and its susceptible recurrent parent (Fla. 8059) upon infection by TSWV. A total of 1,244 differentially expressed genes were identified throughout a disease progression process involving networks of host resistance genes, RNA silencing/antiviral defense genes, and crucial transcriptional and translational regulators. Notable induced genes in Sw-7 include those involved in callose accumulation, lignin deposition, proteolysis process, transcriptional activation/repression, and phosphorylation. Finally, we investigated potential involvement of PR-5 in the Sw-7 resistance. Interestingly, PR-5 overexpressed plants conferred enhanced resistance, resulting in delay in virus accumulation and symptom expression. These findings will facilitate breeding and genetic engineering efforts to incorporate this new source of resistance in tomato for protection against TSWV. |
| first_indexed | 2024-12-20T21:31:18Z |
| format | Article |
| id | doaj.art-62caa9c2837f46b5aa9c62b79c58750c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T21:31:18Z |
| publishDate | 2019-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-62caa9c2837f46b5aa9c62b79c58750c2022-12-21T19:26:03ZengNature PortfolioScientific Reports2045-23222019-05-019111710.1038/s41598-019-44100-xComprehensive transcriptome analysis and functional characterization of PR-5 for its involvement in tomato Sw-7 resistance to tomato spotted wilt tospovirusChellappan Padmanabhan0Qiyue Ma1Reza Shekasteband2Kevin S. Stewart3Samuel F. Hutton4John W. Scott5Zhangjun Fei6Kai-Shu Ling7USDA-Agricultural Research Service, U.S. Vegetable LaboratoryBoyce Thompson Institute, Cornell UniversityUniversity of Florida, IFAS, Gulf Coast Research and Education CenterUSDA-Agricultural Research Service, U.S. Vegetable LaboratoryUniversity of Florida, IFAS, Gulf Coast Research and Education CenterUniversity of Florida, IFAS, Gulf Coast Research and Education CenterBoyce Thompson Institute, Cornell UniversityUSDA-Agricultural Research Service, U.S. Vegetable LaboratoryAbstract Tomato spotted wilt tospovirus (TSWV), one of the most important plant viruses, causes yield losses to many crops including tomato. The current disease management for TSWV is based mainly on breeding tomato cultivars containing the Sw-5 locus. Unfortunately, several Sw-5 resistance-breaking strains of TSWV have been identified. Sw-7 is an alternative locus conferring resistance to a broad range of TSWV strains. In an effort to uncover gene networks that are associated with the Sw-7 resistance, we performed a comparative transcriptome profiling and gene expression analysis between a nearly-isogenic Sw-7 line and its susceptible recurrent parent (Fla. 8059) upon infection by TSWV. A total of 1,244 differentially expressed genes were identified throughout a disease progression process involving networks of host resistance genes, RNA silencing/antiviral defense genes, and crucial transcriptional and translational regulators. Notable induced genes in Sw-7 include those involved in callose accumulation, lignin deposition, proteolysis process, transcriptional activation/repression, and phosphorylation. Finally, we investigated potential involvement of PR-5 in the Sw-7 resistance. Interestingly, PR-5 overexpressed plants conferred enhanced resistance, resulting in delay in virus accumulation and symptom expression. These findings will facilitate breeding and genetic engineering efforts to incorporate this new source of resistance in tomato for protection against TSWV.https://doi.org/10.1038/s41598-019-44100-x |
| spellingShingle | Chellappan Padmanabhan Qiyue Ma Reza Shekasteband Kevin S. Stewart Samuel F. Hutton John W. Scott Zhangjun Fei Kai-Shu Ling Comprehensive transcriptome analysis and functional characterization of PR-5 for its involvement in tomato Sw-7 resistance to tomato spotted wilt tospovirus Scientific Reports |
| title | Comprehensive transcriptome analysis and functional characterization of PR-5 for its involvement in tomato Sw-7 resistance to tomato spotted wilt tospovirus |
| title_full | Comprehensive transcriptome analysis and functional characterization of PR-5 for its involvement in tomato Sw-7 resistance to tomato spotted wilt tospovirus |
| title_fullStr | Comprehensive transcriptome analysis and functional characterization of PR-5 for its involvement in tomato Sw-7 resistance to tomato spotted wilt tospovirus |
| title_full_unstemmed | Comprehensive transcriptome analysis and functional characterization of PR-5 for its involvement in tomato Sw-7 resistance to tomato spotted wilt tospovirus |
| title_short | Comprehensive transcriptome analysis and functional characterization of PR-5 for its involvement in tomato Sw-7 resistance to tomato spotted wilt tospovirus |
| title_sort | comprehensive transcriptome analysis and functional characterization of pr 5 for its involvement in tomato sw 7 resistance to tomato spotted wilt tospovirus |
| url | https://doi.org/10.1038/s41598-019-44100-x |
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