HPLC-DPPH Screening Method for Evaluation of Antioxidant Compounds Extracted from Semen Oroxyli
Semen Oroxyli, derived from the seed of Oroxylum indicum L., is a commonly used Traditional Chinese Medicine with beneficial effects against several respiratory disorders. Antioxidative flavonoids may be partly responsible for its medicinal functions. The aim of this study was to rapidly determine t...
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MDPI AG
2014-04-01
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Online Access: | http://www.mdpi.com/1420-3049/19/4/4409 |
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author | Renyi Yan Yangyang Cao Bin Yang |
author_facet | Renyi Yan Yangyang Cao Bin Yang |
author_sort | Renyi Yan |
collection | DOAJ |
description | Semen Oroxyli, derived from the seed of Oroxylum indicum L., is a commonly used Traditional Chinese Medicine with beneficial effects against several respiratory disorders. Antioxidative flavonoids may be partly responsible for its medicinal functions. The aim of this study was to rapidly determine the antioxidants in Semen Oroxyli based on a HPLC-DPPH method. Four major flavonoids, baicalein-7-O-gentiobioside, baicalein-7-O-glucoside, baicalein, and baicalin, were identified as the active components against DPPH free radicals, which is in accord with the results of our former traditional activity-guided phytochemical study. The oxidative products of the four antioxidant flavonoids were studied in the DPPH spiking HPLC assay, it was suggested that the three active flavonoid glycosides were converted into 5,6-dihydroxy-7-methoxyflavone, which implied that an additional hydroxyl at C-6 in 5,7-dihydroxyflavones plays an important role in the DPPH assay. |
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language | English |
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spelling | doaj.art-62cbb08114ca4b778402fca5d47d417d2022-12-21T21:59:59ZengMDPI AGMolecules1420-30492014-04-011944409441710.3390/molecules19044409molecules19044409HPLC-DPPH Screening Method for Evaluation of Antioxidant Compounds Extracted from Semen OroxyliRenyi Yan0Yangyang Cao1Bin Yang2Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaSemen Oroxyli, derived from the seed of Oroxylum indicum L., is a commonly used Traditional Chinese Medicine with beneficial effects against several respiratory disorders. Antioxidative flavonoids may be partly responsible for its medicinal functions. The aim of this study was to rapidly determine the antioxidants in Semen Oroxyli based on a HPLC-DPPH method. Four major flavonoids, baicalein-7-O-gentiobioside, baicalein-7-O-glucoside, baicalein, and baicalin, were identified as the active components against DPPH free radicals, which is in accord with the results of our former traditional activity-guided phytochemical study. The oxidative products of the four antioxidant flavonoids were studied in the DPPH spiking HPLC assay, it was suggested that the three active flavonoid glycosides were converted into 5,6-dihydroxy-7-methoxyflavone, which implied that an additional hydroxyl at C-6 in 5,7-dihydroxyflavones plays an important role in the DPPH assay.http://www.mdpi.com/1420-3049/19/4/4409Oroxylum indicumflavonoidHPLC-DPPHantioxidantrapid screening |
spellingShingle | Renyi Yan Yangyang Cao Bin Yang HPLC-DPPH Screening Method for Evaluation of Antioxidant Compounds Extracted from Semen Oroxyli Molecules Oroxylum indicum flavonoid HPLC-DPPH antioxidant rapid screening |
title | HPLC-DPPH Screening Method for Evaluation of Antioxidant Compounds Extracted from Semen Oroxyli |
title_full | HPLC-DPPH Screening Method for Evaluation of Antioxidant Compounds Extracted from Semen Oroxyli |
title_fullStr | HPLC-DPPH Screening Method for Evaluation of Antioxidant Compounds Extracted from Semen Oroxyli |
title_full_unstemmed | HPLC-DPPH Screening Method for Evaluation of Antioxidant Compounds Extracted from Semen Oroxyli |
title_short | HPLC-DPPH Screening Method for Evaluation of Antioxidant Compounds Extracted from Semen Oroxyli |
title_sort | hplc dpph screening method for evaluation of antioxidant compounds extracted from semen oroxyli |
topic | Oroxylum indicum flavonoid HPLC-DPPH antioxidant rapid screening |
url | http://www.mdpi.com/1420-3049/19/4/4409 |
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