HPLC-DPPH Screening Method for Evaluation of Antioxidant Compounds Extracted from Semen Oroxyli

Semen Oroxyli, derived from the seed of Oroxylum indicum L., is a commonly used Traditional Chinese Medicine with beneficial effects against several respiratory disorders. Antioxidative flavonoids may be partly responsible for its medicinal functions. The aim of this study was to rapidly determine t...

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Main Authors: Renyi Yan, Yangyang Cao, Bin Yang
Format: Article
Language:English
Published: MDPI AG 2014-04-01
Series:Molecules
Subjects:
Online Access:http://www.mdpi.com/1420-3049/19/4/4409
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author Renyi Yan
Yangyang Cao
Bin Yang
author_facet Renyi Yan
Yangyang Cao
Bin Yang
author_sort Renyi Yan
collection DOAJ
description Semen Oroxyli, derived from the seed of Oroxylum indicum L., is a commonly used Traditional Chinese Medicine with beneficial effects against several respiratory disorders. Antioxidative flavonoids may be partly responsible for its medicinal functions. The aim of this study was to rapidly determine the antioxidants in Semen Oroxyli based on a HPLC-DPPH method. Four major flavonoids, baicalein-7-O-gentiobioside, baicalein-7-O-glucoside, baicalein, and baicalin, were identified as the active components against DPPH free radicals, which is in accord with the results of our former traditional activity-guided phytochemical study. The oxidative products of the four antioxidant flavonoids were studied in the DPPH spiking HPLC assay, it was suggested that the three active flavonoid glycosides were converted into 5,6-dihydroxy-7-methoxyflavone, which implied that an additional hydroxyl at C-6 in 5,7-dihydroxyflavones plays an important role in the DPPH assay.
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spelling doaj.art-62cbb08114ca4b778402fca5d47d417d2022-12-21T21:59:59ZengMDPI AGMolecules1420-30492014-04-011944409441710.3390/molecules19044409molecules19044409HPLC-DPPH Screening Method for Evaluation of Antioxidant Compounds Extracted from Semen OroxyliRenyi Yan0Yangyang Cao1Bin Yang2Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaSemen Oroxyli, derived from the seed of Oroxylum indicum L., is a commonly used Traditional Chinese Medicine with beneficial effects against several respiratory disorders. Antioxidative flavonoids may be partly responsible for its medicinal functions. The aim of this study was to rapidly determine the antioxidants in Semen Oroxyli based on a HPLC-DPPH method. Four major flavonoids, baicalein-7-O-gentiobioside, baicalein-7-O-glucoside, baicalein, and baicalin, were identified as the active components against DPPH free radicals, which is in accord with the results of our former traditional activity-guided phytochemical study. The oxidative products of the four antioxidant flavonoids were studied in the DPPH spiking HPLC assay, it was suggested that the three active flavonoid glycosides were converted into 5,6-dihydroxy-7-methoxyflavone, which implied that an additional hydroxyl at C-6 in 5,7-dihydroxyflavones plays an important role in the DPPH assay.http://www.mdpi.com/1420-3049/19/4/4409Oroxylum indicumflavonoidHPLC-DPPHantioxidantrapid screening
spellingShingle Renyi Yan
Yangyang Cao
Bin Yang
HPLC-DPPH Screening Method for Evaluation of Antioxidant Compounds Extracted from Semen Oroxyli
Molecules
Oroxylum indicum
flavonoid
HPLC-DPPH
antioxidant
rapid screening
title HPLC-DPPH Screening Method for Evaluation of Antioxidant Compounds Extracted from Semen Oroxyli
title_full HPLC-DPPH Screening Method for Evaluation of Antioxidant Compounds Extracted from Semen Oroxyli
title_fullStr HPLC-DPPH Screening Method for Evaluation of Antioxidant Compounds Extracted from Semen Oroxyli
title_full_unstemmed HPLC-DPPH Screening Method for Evaluation of Antioxidant Compounds Extracted from Semen Oroxyli
title_short HPLC-DPPH Screening Method for Evaluation of Antioxidant Compounds Extracted from Semen Oroxyli
title_sort hplc dpph screening method for evaluation of antioxidant compounds extracted from semen oroxyli
topic Oroxylum indicum
flavonoid
HPLC-DPPH
antioxidant
rapid screening
url http://www.mdpi.com/1420-3049/19/4/4409
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