Quercetin attenuates inflammation in LPS‐induced lung epithelial cells via the Nrf2 signaling pathway
Abstract Background Pneumonia is the leading cause of death among children under five, and kill almost two million children each year. Quercetin, a flavonoid polyphenolic compound, exerts many beneficial biological activities, including anti‐inflammatory functions. Our study aimed to investigate the...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-02-01
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| Series: | Immunity, Inflammation and Disease |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/iid3.1185 |
| _version_ | 1797292133716066304 |
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| author | Pengju Lv Pengli Han Yuanbo Cui Qiusheng Chen Wei Cao |
| author_facet | Pengju Lv Pengli Han Yuanbo Cui Qiusheng Chen Wei Cao |
| author_sort | Pengju Lv |
| collection | DOAJ |
| description | Abstract Background Pneumonia is the leading cause of death among children under five, and kill almost two million children each year. Quercetin, a flavonoid polyphenolic compound, exerts many beneficial biological activities, including anti‐inflammatory functions. Our study aimed to investigate the possibility of quercetin as a therapeutic agent for pneumonia and its role in the inflammatory response induced by lipopolysaccharide (LPS). Methods LPS induced human alveolar epithelial cell A549 as a lung inflammation model in vitro. The effects of quercetin on the production of cytokines and the expression of related‐proteins were detected by Enzyme‐Linked ImmunoSorbent Assay and Western Blot, respectively. Cell Counting Kit‐8 assay was used to detect cell viability. flow cytometry was used to measure cell apoptosis. NO levels were also analyzed through NO kit. Results Our results found that quercetin attenuated the release of IL‐1β, IL‐6, PGE2, and nitrite in LPS‐induced A549 cells. In addition, quercetin inhibits cell apoptosis and relieves ROS generation in LPS‐induced A549 cells. Quercetin also inhibits LPS‐induced NF‐κB activation. They have upregulated the expression of nuclear factor erythroid 2 (Nrf2) and HO‐1. Conclusion In conclusion, these results suggested that quercetin attenuates LPS‐induced inflammation in A549 by activating the Nrf2 signaling pathway. |
| first_indexed | 2024-03-07T19:47:20Z |
| format | Article |
| id | doaj.art-62cebdc893574984b75b2e19c42459a1 |
| institution | Directory Open Access Journal |
| issn | 2050-4527 |
| language | English |
| last_indexed | 2024-03-07T19:47:20Z |
| publishDate | 2024-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Immunity, Inflammation and Disease |
| spelling | doaj.art-62cebdc893574984b75b2e19c42459a12024-02-28T18:18:31ZengWileyImmunity, Inflammation and Disease2050-45272024-02-01122n/an/a10.1002/iid3.1185Quercetin attenuates inflammation in LPS‐induced lung epithelial cells via the Nrf2 signaling pathwayPengju Lv0Pengli Han1Yuanbo Cui2Qiusheng Chen3Wei Cao4Translational Medical Center Zhengzhou Central Hospital Affiliated to Zhengzhou University Zhengzhou ChinaTranslational Medical Center Zhengzhou Central Hospital Affiliated to Zhengzhou University Zhengzhou ChinaTranslational Medical Center Zhengzhou Central Hospital Affiliated to Zhengzhou University Zhengzhou ChinaDepartment of Pulmonary and Critical Care Medicine Zhengzhou Central Hospital Affiliated to Zhengzhou University Zhengzhou ChinaTranslational Medical Center Zhengzhou Central Hospital Affiliated to Zhengzhou University Zhengzhou ChinaAbstract Background Pneumonia is the leading cause of death among children under five, and kill almost two million children each year. Quercetin, a flavonoid polyphenolic compound, exerts many beneficial biological activities, including anti‐inflammatory functions. Our study aimed to investigate the possibility of quercetin as a therapeutic agent for pneumonia and its role in the inflammatory response induced by lipopolysaccharide (LPS). Methods LPS induced human alveolar epithelial cell A549 as a lung inflammation model in vitro. The effects of quercetin on the production of cytokines and the expression of related‐proteins were detected by Enzyme‐Linked ImmunoSorbent Assay and Western Blot, respectively. Cell Counting Kit‐8 assay was used to detect cell viability. flow cytometry was used to measure cell apoptosis. NO levels were also analyzed through NO kit. Results Our results found that quercetin attenuated the release of IL‐1β, IL‐6, PGE2, and nitrite in LPS‐induced A549 cells. In addition, quercetin inhibits cell apoptosis and relieves ROS generation in LPS‐induced A549 cells. Quercetin also inhibits LPS‐induced NF‐κB activation. They have upregulated the expression of nuclear factor erythroid 2 (Nrf2) and HO‐1. Conclusion In conclusion, these results suggested that quercetin attenuates LPS‐induced inflammation in A549 by activating the Nrf2 signaling pathway.https://doi.org/10.1002/iid3.1185inflammatory cytokineLPSNrf2pneumoniaquercetin |
| spellingShingle | Pengju Lv Pengli Han Yuanbo Cui Qiusheng Chen Wei Cao Quercetin attenuates inflammation in LPS‐induced lung epithelial cells via the Nrf2 signaling pathway Immunity, Inflammation and Disease inflammatory cytokine LPS Nrf2 pneumonia quercetin |
| title | Quercetin attenuates inflammation in LPS‐induced lung epithelial cells via the Nrf2 signaling pathway |
| title_full | Quercetin attenuates inflammation in LPS‐induced lung epithelial cells via the Nrf2 signaling pathway |
| title_fullStr | Quercetin attenuates inflammation in LPS‐induced lung epithelial cells via the Nrf2 signaling pathway |
| title_full_unstemmed | Quercetin attenuates inflammation in LPS‐induced lung epithelial cells via the Nrf2 signaling pathway |
| title_short | Quercetin attenuates inflammation in LPS‐induced lung epithelial cells via the Nrf2 signaling pathway |
| title_sort | quercetin attenuates inflammation in lps induced lung epithelial cells via the nrf2 signaling pathway |
| topic | inflammatory cytokine LPS Nrf2 pneumonia quercetin |
| url | https://doi.org/10.1002/iid3.1185 |
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