Evaluation of Crossability between <i>Nicotiana benthamiana</i> and <i>Nicotiana excelsior</i>
Wild tobacco species in the <i>Nicotiana</i> section <i>Suaveolentes</i> are promising genetic resources to introduce their disease resistance to cultivated tobacco, <i>Nicotiana tabacum</i>. However, hybrid lethality is observed in hybrid seedlings from crosses b...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/11/12/2583 |
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| author | Takahiro Tezuka Naoto Kitamura Masanori Yanase Toshinobu Morikawa |
| author_facet | Takahiro Tezuka Naoto Kitamura Masanori Yanase Toshinobu Morikawa |
| author_sort | Takahiro Tezuka |
| collection | DOAJ |
| description | Wild tobacco species in the <i>Nicotiana</i> section <i>Suaveolentes</i> are promising genetic resources to introduce their disease resistance to cultivated tobacco, <i>Nicotiana tabacum</i>. However, hybrid lethality is observed in hybrid seedlings from crosses between most <i>Suaveolentes</i> species and <i>N. tabacum</i>. In particular, <i>N. benthamiana</i> belonging to the section <i>Suaveolentes</i> produces only viable hybrids after crossing with <i>N. tabacum</i>. In the present study, crossability between <i>N. benthamiana</i> and <i>N. excelsior</i> (section <i>Suaveolentes</i>) was investigated to test the possible usefulness of <i>N. benthamiana</i> as the bridge parent to transfer desirable genes of <i>N. excelsior</i> to <i>N. tabacum</i> via bridge crossing. After reciprocal crosses using three accessions of <i>N. benthamiana</i> and <i>N. excelsior</i> each, several crossing barriers such as cross-incompatibility, seed abortion, and male and female hybrid sterility were observed. Although reciprocal hybrids between <i>N. benthamiana</i> and <i>N. excelsior</i> showed a high degree of chromosome pairing in meiosis, univalents and multivalents, as well as chromosome bridges and lagging chromosomes, were observed. These meiotic abnormalities were thought to cause hybrid sterility. The possible usefulness of reciprocal hybrids between <i>N. benthamiana</i> and <i>N. excelsior</i> is discussed. |
| first_indexed | 2024-03-10T04:40:30Z |
| format | Article |
| id | doaj.art-62d211328ed9449d810169bcbe9ebb5a |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T04:40:30Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-62d211328ed9449d810169bcbe9ebb5a2023-11-23T03:24:12ZengMDPI AGAgronomy2073-43952021-12-011112258310.3390/agronomy11122583Evaluation of Crossability between <i>Nicotiana benthamiana</i> and <i>Nicotiana excelsior</i>Takahiro Tezuka0Naoto Kitamura1Masanori Yanase2Toshinobu Morikawa3Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai 599-8531, Osaka, JapanGraduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai 599-8531, Osaka, JapanGraduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai 599-8531, Osaka, JapanGraduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai 599-8531, Osaka, JapanWild tobacco species in the <i>Nicotiana</i> section <i>Suaveolentes</i> are promising genetic resources to introduce their disease resistance to cultivated tobacco, <i>Nicotiana tabacum</i>. However, hybrid lethality is observed in hybrid seedlings from crosses between most <i>Suaveolentes</i> species and <i>N. tabacum</i>. In particular, <i>N. benthamiana</i> belonging to the section <i>Suaveolentes</i> produces only viable hybrids after crossing with <i>N. tabacum</i>. In the present study, crossability between <i>N. benthamiana</i> and <i>N. excelsior</i> (section <i>Suaveolentes</i>) was investigated to test the possible usefulness of <i>N. benthamiana</i> as the bridge parent to transfer desirable genes of <i>N. excelsior</i> to <i>N. tabacum</i> via bridge crossing. After reciprocal crosses using three accessions of <i>N. benthamiana</i> and <i>N. excelsior</i> each, several crossing barriers such as cross-incompatibility, seed abortion, and male and female hybrid sterility were observed. Although reciprocal hybrids between <i>N. benthamiana</i> and <i>N. excelsior</i> showed a high degree of chromosome pairing in meiosis, univalents and multivalents, as well as chromosome bridges and lagging chromosomes, were observed. These meiotic abnormalities were thought to cause hybrid sterility. The possible usefulness of reciprocal hybrids between <i>N. benthamiana</i> and <i>N. excelsior</i> is discussed.https://www.mdpi.com/2073-4395/11/12/2583chromosome pairingcross-incompatibilitycrossabilityhybrid sterilityinterspecific hybrid<i>Nicotiana benthamiana</i> |
| spellingShingle | Takahiro Tezuka Naoto Kitamura Masanori Yanase Toshinobu Morikawa Evaluation of Crossability between <i>Nicotiana benthamiana</i> and <i>Nicotiana excelsior</i> Agronomy chromosome pairing cross-incompatibility crossability hybrid sterility interspecific hybrid <i>Nicotiana benthamiana</i> |
| title | Evaluation of Crossability between <i>Nicotiana benthamiana</i> and <i>Nicotiana excelsior</i> |
| title_full | Evaluation of Crossability between <i>Nicotiana benthamiana</i> and <i>Nicotiana excelsior</i> |
| title_fullStr | Evaluation of Crossability between <i>Nicotiana benthamiana</i> and <i>Nicotiana excelsior</i> |
| title_full_unstemmed | Evaluation of Crossability between <i>Nicotiana benthamiana</i> and <i>Nicotiana excelsior</i> |
| title_short | Evaluation of Crossability between <i>Nicotiana benthamiana</i> and <i>Nicotiana excelsior</i> |
| title_sort | evaluation of crossability between i nicotiana benthamiana i and i nicotiana excelsior i |
| topic | chromosome pairing cross-incompatibility crossability hybrid sterility interspecific hybrid <i>Nicotiana benthamiana</i> |
| url | https://www.mdpi.com/2073-4395/11/12/2583 |
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