Investigating the Effects of Exposure to Continuous White Noise on SLC26A4 Gene Expression Levels in Male Rat Cochlea
Background and purpose: Irreversible damage to the inner ear is known as noise-induced hearing loss (NIHL). Exposure to excessive noise can affect the expression of genes in molecules involved in development of NIHL. SLC26A4 gene or PDS is responsible for causing both syndromic and non-syndromic dea...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2021-07-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jmums.mazums.ac.ir/article-1-16305-en.html |
| _version_ | 1828055071586254848 |
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| author | Maryam Mirzaei Hotkani Kolsoum Inanloorahatloo Mohammad Reza MonazzamEsmaeilpoor3 Amir Abbasi Garmaroudi Monireh Khadem |
| author_facet | Maryam Mirzaei Hotkani Kolsoum Inanloorahatloo Mohammad Reza MonazzamEsmaeilpoor3 Amir Abbasi Garmaroudi Monireh Khadem |
| author_sort | Maryam Mirzaei Hotkani |
| collection | DOAJ |
| description | Background and purpose: Irreversible damage to the inner ear is known as noise-induced hearing loss (NIHL). Exposure to excessive noise can affect the expression of genes in molecules involved in development of NIHL. SLC26A4 gene or PDS is responsible for causing both syndromic and non-syndromic deafness and is located at DFNB site. The aim of this study was to investigate the expression level of this gene due to continuous white noise exposure.
Materials and methods: In this experimental study, 10 healthy male Wistar rats were divided into exposure group (n=6) and control group (n=4). Exposur group was exposed to continuous white noise (8hr/day, 6days, SPL=110±5dB) in noise chambers. Cochlear biopsy was done 1 hour (n=5) and 1 week (n=5) after exposure and then, RNA extraction, cDNA synthesis and Real-Time PCR analysis were done. In addition, histological analyzes were performed. Data analysis was done using One-Way ANOVA (95% CI diff.).
Results: At both 1 hour and 1 week after exposure, the expression of SLC26A4 gene decreased significantly (P= 0.0005 and P= 0.0003, respectively). The fold changes compared to the control group (=1) were 0.0733 and 0.00712, respectively. Histological images illustrated serious damage to the cochlea.
Conclusion: Sensorineural deafness will happen if exposure to intense noise continues and subsequently down-regulation of SLC26A4 in the cochlea occur during longer discontinuation and in case of expression level failure to return to its basal level. |
| first_indexed | 2024-04-10T20:32:50Z |
| format | Article |
| id | doaj.art-62d3fc781c9d46e4878dec51069e54b2 |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-10T20:32:50Z |
| publishDate | 2021-07-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-62d3fc781c9d46e4878dec51069e54b22023-01-25T05:14:26ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792021-07-01311982839Investigating the Effects of Exposure to Continuous White Noise on SLC26A4 Gene Expression Levels in Male Rat CochleaMaryam Mirzaei Hotkani0Kolsoum Inanloorahatloo1Mohammad Reza MonazzamEsmaeilpoor32Amir Abbasi Garmaroudi3Monireh Khadem4 MSc in Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Assistant Professor, Department of Cell and Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran Professor, Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran MSc Student in Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Assistant Professor, Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Background and purpose: Irreversible damage to the inner ear is known as noise-induced hearing loss (NIHL). Exposure to excessive noise can affect the expression of genes in molecules involved in development of NIHL. SLC26A4 gene or PDS is responsible for causing both syndromic and non-syndromic deafness and is located at DFNB site. The aim of this study was to investigate the expression level of this gene due to continuous white noise exposure. Materials and methods: In this experimental study, 10 healthy male Wistar rats were divided into exposure group (n=6) and control group (n=4). Exposur group was exposed to continuous white noise (8hr/day, 6days, SPL=110±5dB) in noise chambers. Cochlear biopsy was done 1 hour (n=5) and 1 week (n=5) after exposure and then, RNA extraction, cDNA synthesis and Real-Time PCR analysis were done. In addition, histological analyzes were performed. Data analysis was done using One-Way ANOVA (95% CI diff.). Results: At both 1 hour and 1 week after exposure, the expression of SLC26A4 gene decreased significantly (P= 0.0005 and P= 0.0003, respectively). The fold changes compared to the control group (=1) were 0.0733 and 0.00712, respectively. Histological images illustrated serious damage to the cochlea. Conclusion: Sensorineural deafness will happen if exposure to intense noise continues and subsequently down-regulation of SLC26A4 in the cochlea occur during longer discontinuation and in case of expression level failure to return to its basal level.http://jmums.mazums.ac.ir/article-1-16305-en.htmlnoisegene expressionslc26a4 genecochleanoise-induced hearing loss |
| spellingShingle | Maryam Mirzaei Hotkani Kolsoum Inanloorahatloo Mohammad Reza MonazzamEsmaeilpoor3 Amir Abbasi Garmaroudi Monireh Khadem Investigating the Effects of Exposure to Continuous White Noise on SLC26A4 Gene Expression Levels in Male Rat Cochlea Journal of Mazandaran University of Medical Sciences noise gene expression slc26a4 gene cochlea noise-induced hearing loss |
| title | Investigating the Effects of Exposure to Continuous White Noise on SLC26A4 Gene Expression Levels in Male Rat Cochlea |
| title_full | Investigating the Effects of Exposure to Continuous White Noise on SLC26A4 Gene Expression Levels in Male Rat Cochlea |
| title_fullStr | Investigating the Effects of Exposure to Continuous White Noise on SLC26A4 Gene Expression Levels in Male Rat Cochlea |
| title_full_unstemmed | Investigating the Effects of Exposure to Continuous White Noise on SLC26A4 Gene Expression Levels in Male Rat Cochlea |
| title_short | Investigating the Effects of Exposure to Continuous White Noise on SLC26A4 Gene Expression Levels in Male Rat Cochlea |
| title_sort | investigating the effects of exposure to continuous white noise on slc26a4 gene expression levels in male rat cochlea |
| topic | noise gene expression slc26a4 gene cochlea noise-induced hearing loss |
| url | http://jmums.mazums.ac.ir/article-1-16305-en.html |
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