The anti-inflammatory regulation of calcitonin gene-related peptide in mouse Aspergillus fumigatus keratitis
AIM: To analyze the impact of calcitonin gene-related peptide (CGRP) in mouse keratitis after Aspergillus fumigatus (A. fumigatus) infection. METHODS: C57BL/6 mice were treated subconjunctivally with different concentrations of exogenous CGRP, and BALB/c mice were treated with CGRP8-37 (a CGRP anta...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2020-05-01
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| Series: | International Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://www.ijo.cn/en_publish/2020/5/20200502.pdf |
| _version_ | 1819093803584192512 |
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| author | Ming Xu Cui Li Gui-Qiu Zhao Jing Lin Min Yin Heng-Rui Zheng Li Zhang Meng-Qi Wu |
| author_facet | Ming Xu Cui Li Gui-Qiu Zhao Jing Lin Min Yin Heng-Rui Zheng Li Zhang Meng-Qi Wu |
| author_sort | Ming Xu |
| collection | DOAJ |
| description | AIM: To analyze the impact of calcitonin gene-related peptide (CGRP) in mouse keratitis after Aspergillus fumigatus (A. fumigatus) infection.
METHODS: C57BL/6 mice were treated subconjunctivally with different concentrations of exogenous CGRP, and BALB/c mice were treated with CGRP8-37 (a CGRP antagonist) before corneas were infected with A. fumigatus. The cornea was assessed under the slit-lamp and the clinical score was recorded. The mRNA levels of IL-1β, TNF-α, IL-6, and MIP-2 were detected by quantitative real-time polymerase chain reaction (PCR), while the protein level of IL-1β was determined by Western blotting. In vitro, RAW264.7 cells were used to investigate NLRP3 and IL-1β expression induced by A. fumigatus after the pretreatment of exogenous CGRP or CGRP8-37. Cytokines expression in RAW264.7 cells was evaluated by real-time PCR and Western blotting.
RESULTS: Using exogenous CGRP resulted in down-regulated synthesis of IL-1β and MIP-2 stimulated by A. fumigatus in C57BL/6 mice keratitis, and the synthesis of IL-1β, MIP-2 and IL-6 was up-regulated in BALB/c mice corneas after the pretreatment with CGRP8-37. Pretreatment with exogenous CGRP and CGRP8-37 did not influence TNF-α mRNA levels either in BALB/c or C57BL/6 mice keratitis. The levels of NLRP3 and IL-1β were both reduced in A. fumigatus stimulated-macrophages after treatment with exogenous CGRP. And CGRP8-37 pretreatment would increase NLRP3 and IL-1β levels.
CONCLUSION: CGRP may alleviate the inflammatory reaction in mice keratitis after infection with A. fumigatus. The anti-inflammatory effect may be related to the inhibition of NLRP3 expression by CGRP. |
| first_indexed | 2024-12-21T23:17:19Z |
| format | Article |
| id | doaj.art-62d48309cb6546219ca744b34674e5cb |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-12-21T23:17:19Z |
| publishDate | 2020-05-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-62d48309cb6546219ca744b34674e5cb2022-12-21T18:46:53ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982020-05-0113570170710.18240/ijo.2020.05.02The anti-inflammatory regulation of calcitonin gene-related peptide in mouse Aspergillus fumigatus keratitisMing Xu0Cui Li1Gui-Qiu Zhao2Jing Lin3Min Yin4Heng-Rui Zheng5Li Zhang6Meng-Qi Wu7Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, ChinaDepartment of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, ChinaDepartment of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, ChinaDepartment of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, ChinaDepartment of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, ChinaDepartment of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, ChinaDepartment of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, ChinaDepartment of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, ChinaAIM: To analyze the impact of calcitonin gene-related peptide (CGRP) in mouse keratitis after Aspergillus fumigatus (A. fumigatus) infection. METHODS: C57BL/6 mice were treated subconjunctivally with different concentrations of exogenous CGRP, and BALB/c mice were treated with CGRP8-37 (a CGRP antagonist) before corneas were infected with A. fumigatus. The cornea was assessed under the slit-lamp and the clinical score was recorded. The mRNA levels of IL-1β, TNF-α, IL-6, and MIP-2 were detected by quantitative real-time polymerase chain reaction (PCR), while the protein level of IL-1β was determined by Western blotting. In vitro, RAW264.7 cells were used to investigate NLRP3 and IL-1β expression induced by A. fumigatus after the pretreatment of exogenous CGRP or CGRP8-37. Cytokines expression in RAW264.7 cells was evaluated by real-time PCR and Western blotting. RESULTS: Using exogenous CGRP resulted in down-regulated synthesis of IL-1β and MIP-2 stimulated by A. fumigatus in C57BL/6 mice keratitis, and the synthesis of IL-1β, MIP-2 and IL-6 was up-regulated in BALB/c mice corneas after the pretreatment with CGRP8-37. Pretreatment with exogenous CGRP and CGRP8-37 did not influence TNF-α mRNA levels either in BALB/c or C57BL/6 mice keratitis. The levels of NLRP3 and IL-1β were both reduced in A. fumigatus stimulated-macrophages after treatment with exogenous CGRP. And CGRP8-37 pretreatment would increase NLRP3 and IL-1β levels. CONCLUSION: CGRP may alleviate the inflammatory reaction in mice keratitis after infection with A. fumigatus. The anti-inflammatory effect may be related to the inhibition of NLRP3 expression by CGRP.http://www.ijo.cn/en_publish/2020/5/20200502.pdfcalcitonin gene-related peptidefungal keratitisnlrp3mice |
| spellingShingle | Ming Xu Cui Li Gui-Qiu Zhao Jing Lin Min Yin Heng-Rui Zheng Li Zhang Meng-Qi Wu The anti-inflammatory regulation of calcitonin gene-related peptide in mouse Aspergillus fumigatus keratitis International Journal of Ophthalmology calcitonin gene-related peptide fungal keratitis nlrp3 mice |
| title | The anti-inflammatory regulation of calcitonin gene-related peptide in mouse Aspergillus fumigatus keratitis |
| title_full | The anti-inflammatory regulation of calcitonin gene-related peptide in mouse Aspergillus fumigatus keratitis |
| title_fullStr | The anti-inflammatory regulation of calcitonin gene-related peptide in mouse Aspergillus fumigatus keratitis |
| title_full_unstemmed | The anti-inflammatory regulation of calcitonin gene-related peptide in mouse Aspergillus fumigatus keratitis |
| title_short | The anti-inflammatory regulation of calcitonin gene-related peptide in mouse Aspergillus fumigatus keratitis |
| title_sort | anti inflammatory regulation of calcitonin gene related peptide in mouse aspergillus fumigatus keratitis |
| topic | calcitonin gene-related peptide fungal keratitis nlrp3 mice |
| url | http://www.ijo.cn/en_publish/2020/5/20200502.pdf |
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