Protein composition of the occlusion bodies of Epinotia aporema granulovirus.
Within family Baculoviridae, members of the Betabaculovirus genus are employed as biocontrol agents against lepidopteran pests, either alone or in combination with selected members of the Alphabaculovirus genus. Epinotia aporema granulovirus (EpapGV) is a fast killing betabaculovirus that infects th...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0207735 |
| _version_ | 1818925383631765504 |
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| author | Tomás Masson María Laura Fabre María Leticia Ferrelli Matías Luis Pidre Víctor Romanowski |
| author_facet | Tomás Masson María Laura Fabre María Leticia Ferrelli Matías Luis Pidre Víctor Romanowski |
| author_sort | Tomás Masson |
| collection | DOAJ |
| description | Within family Baculoviridae, members of the Betabaculovirus genus are employed as biocontrol agents against lepidopteran pests, either alone or in combination with selected members of the Alphabaculovirus genus. Epinotia aporema granulovirus (EpapGV) is a fast killing betabaculovirus that infects the bean shoot borer (E. aporema) and is a promising biopesticide. Because occlusion bodies (OBs) play a key role in baculovirus horizontal transmission, we investigated the composition of EpapGV OBs. Using mass spectrometry-based proteomics we could identify 56 proteins that are included in the OBs during the final stages of larval infection. Our data provides experimental validation of several annotated hypothetical coding sequences. Proteogenomic mapping against genomic sequence detected a previously unannotated ac110-like core gene and a putative translation fusion product of ORFs epap48 and epap49. Comparative studies of the proteomes available for the family Baculoviridae highlight the conservation of core gene products as parts of the occluded virion. Two proteins specific for betabaculoviruses (Epap48 and Epap95) are incorporated into OBs. Moreover, quantification based on emPAI values showed that Epap95 is one of the most abundant components of EpapGV OBs. |
| first_indexed | 2024-12-20T02:40:21Z |
| format | Article |
| id | doaj.art-62d6eb0844764d8a9c83880927892587 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T02:40:21Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-62d6eb0844764d8a9c838809278925872022-12-21T19:56:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01142e020773510.1371/journal.pone.0207735Protein composition of the occlusion bodies of Epinotia aporema granulovirus.Tomás MassonMaría Laura FabreMaría Leticia FerrelliMatías Luis PidreVíctor RomanowskiWithin family Baculoviridae, members of the Betabaculovirus genus are employed as biocontrol agents against lepidopteran pests, either alone or in combination with selected members of the Alphabaculovirus genus. Epinotia aporema granulovirus (EpapGV) is a fast killing betabaculovirus that infects the bean shoot borer (E. aporema) and is a promising biopesticide. Because occlusion bodies (OBs) play a key role in baculovirus horizontal transmission, we investigated the composition of EpapGV OBs. Using mass spectrometry-based proteomics we could identify 56 proteins that are included in the OBs during the final stages of larval infection. Our data provides experimental validation of several annotated hypothetical coding sequences. Proteogenomic mapping against genomic sequence detected a previously unannotated ac110-like core gene and a putative translation fusion product of ORFs epap48 and epap49. Comparative studies of the proteomes available for the family Baculoviridae highlight the conservation of core gene products as parts of the occluded virion. Two proteins specific for betabaculoviruses (Epap48 and Epap95) are incorporated into OBs. Moreover, quantification based on emPAI values showed that Epap95 is one of the most abundant components of EpapGV OBs.https://doi.org/10.1371/journal.pone.0207735 |
| spellingShingle | Tomás Masson María Laura Fabre María Leticia Ferrelli Matías Luis Pidre Víctor Romanowski Protein composition of the occlusion bodies of Epinotia aporema granulovirus. PLoS ONE |
| title | Protein composition of the occlusion bodies of Epinotia aporema granulovirus. |
| title_full | Protein composition of the occlusion bodies of Epinotia aporema granulovirus. |
| title_fullStr | Protein composition of the occlusion bodies of Epinotia aporema granulovirus. |
| title_full_unstemmed | Protein composition of the occlusion bodies of Epinotia aporema granulovirus. |
| title_short | Protein composition of the occlusion bodies of Epinotia aporema granulovirus. |
| title_sort | protein composition of the occlusion bodies of epinotia aporema granulovirus |
| url | https://doi.org/10.1371/journal.pone.0207735 |
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