Analysis of the ergosterol biosynthesis pathway cloning, molecular characterization and phylogeny of lanosterol 14α-demethylase (ERG11) gene of Moniliophthora perniciosa
The phytopathogenic fungus Moniliophthora perniciosa (Stahel) Aime & Philips-Mora, causal agent of witches' broom disease of cocoa, causes countless damage to cocoa production in Brazil. Molecular studies have attempted to identify genes that play important roles in fungal survival and viru...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Genética
2014-11-01
|
| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500011&tlng=en |
| _version_ | 1831715626741137408 |
|---|---|
| author | Geruza de Oliveira Ceita Laurival Antônio Vilas-Boas Marcelo Santos Castilho Marcelo Falsarella Carazzolle Carlos Priminho Pirovani Alessandra Selbach-Schnadelbach Karina Peres Gramacho Pablo Ivan Pereira Ramos Luciana Veiga Barbosa Gonçalo Amarante Guimarães Pereira Aristóteles Góes-Neto |
| author_facet | Geruza de Oliveira Ceita Laurival Antônio Vilas-Boas Marcelo Santos Castilho Marcelo Falsarella Carazzolle Carlos Priminho Pirovani Alessandra Selbach-Schnadelbach Karina Peres Gramacho Pablo Ivan Pereira Ramos Luciana Veiga Barbosa Gonçalo Amarante Guimarães Pereira Aristóteles Góes-Neto |
| author_sort | Geruza de Oliveira Ceita |
| collection | DOAJ |
| description | The phytopathogenic fungus Moniliophthora perniciosa (Stahel) Aime & Philips-Mora, causal agent of witches' broom disease of cocoa, causes countless damage to cocoa production in Brazil. Molecular studies have attempted to identify genes that play important roles in fungal survival and virulence. In this study, sequences deposited in the M. perniciosa Genome Sequencing Project database were analyzed to identify potential biological targets. For the first time, the ergosterol biosynthetic pathway in M. perniciosa was studied and the lanosterol 14α-demethylase gene (ERG11) that encodes the main enzyme of this pathway and is a target for fungicides was cloned, characterized molecularly and its phylogeny analyzed. ERG11 genomic DNA and cDNA were characterized and sequence analysis of the ERG11 protein identified highly conserved domains typical of this enzyme, such as SRS1, SRS4, EXXR and the heme-binding region (HBR). Comparison of the protein sequences and phylogenetic analysis revealed that the M. perniciosa enzyme was most closely related to that of Coprinopsis cinerea. |
| first_indexed | 2024-12-21T00:22:35Z |
| format | Article |
| id | doaj.art-62d811c9e70647ba9eb0b36ee1254877 |
| institution | Directory Open Access Journal |
| issn | 1678-4685 |
| language | English |
| last_indexed | 2024-12-21T00:22:35Z |
| publishDate | 2014-11-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-62d811c9e70647ba9eb0b36ee12548772022-12-21T19:22:03ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1678-46852014-11-0137468369310.1590/S1415-47572014005000017Analysis of the ergosterol biosynthesis pathway cloning, molecular characterization and phylogeny of lanosterol 14α-demethylase (ERG11) gene of Moniliophthora perniciosaGeruza de Oliveira Ceita0Laurival Antônio Vilas-Boas1Marcelo Santos Castilho2Marcelo Falsarella Carazzolle3Carlos Priminho Pirovani4Alessandra Selbach-Schnadelbach5Karina Peres Gramacho6Pablo Ivan Pereira Ramos7Luciana Veiga Barbosa8Gonçalo Amarante Guimarães Pereira9Aristóteles Góes-Neto10Universidade Estadual de Feira de SantanaUniversidade Estadual de LondrinaUniversidade Federal da BahiaUniversidade Estadual de CampinasUniversidade Estadual de Santa CruzUniversidade Federal da BahiaCentro de Pesquisas do CacauUniversidade Federal da BahiaUniversidade Federal da BahiaUniversidade Estadual de CampinasUniversidade Estadual de Feira de SantanaThe phytopathogenic fungus Moniliophthora perniciosa (Stahel) Aime & Philips-Mora, causal agent of witches' broom disease of cocoa, causes countless damage to cocoa production in Brazil. Molecular studies have attempted to identify genes that play important roles in fungal survival and virulence. In this study, sequences deposited in the M. perniciosa Genome Sequencing Project database were analyzed to identify potential biological targets. For the first time, the ergosterol biosynthetic pathway in M. perniciosa was studied and the lanosterol 14α-demethylase gene (ERG11) that encodes the main enzyme of this pathway and is a target for fungicides was cloned, characterized molecularly and its phylogeny analyzed. ERG11 genomic DNA and cDNA were characterized and sequence analysis of the ERG11 protein identified highly conserved domains typical of this enzyme, such as SRS1, SRS4, EXXR and the heme-binding region (HBR). Comparison of the protein sequences and phylogenetic analysis revealed that the M. perniciosa enzyme was most closely related to that of Coprinopsis cinerea.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500011&tlng=enBasidiomycotafungusergosterolTheobroma cacao |
| spellingShingle | Geruza de Oliveira Ceita Laurival Antônio Vilas-Boas Marcelo Santos Castilho Marcelo Falsarella Carazzolle Carlos Priminho Pirovani Alessandra Selbach-Schnadelbach Karina Peres Gramacho Pablo Ivan Pereira Ramos Luciana Veiga Barbosa Gonçalo Amarante Guimarães Pereira Aristóteles Góes-Neto Analysis of the ergosterol biosynthesis pathway cloning, molecular characterization and phylogeny of lanosterol 14α-demethylase (ERG11) gene of Moniliophthora perniciosa Genetics and Molecular Biology Basidiomycota fungus ergosterol Theobroma cacao |
| title | Analysis of the ergosterol biosynthesis pathway cloning, molecular characterization and phylogeny of lanosterol 14α-demethylase (ERG11) gene of Moniliophthora perniciosa |
| title_full | Analysis of the ergosterol biosynthesis pathway cloning, molecular characterization and phylogeny of lanosterol 14α-demethylase (ERG11) gene of Moniliophthora perniciosa |
| title_fullStr | Analysis of the ergosterol biosynthesis pathway cloning, molecular characterization and phylogeny of lanosterol 14α-demethylase (ERG11) gene of Moniliophthora perniciosa |
| title_full_unstemmed | Analysis of the ergosterol biosynthesis pathway cloning, molecular characterization and phylogeny of lanosterol 14α-demethylase (ERG11) gene of Moniliophthora perniciosa |
| title_short | Analysis of the ergosterol biosynthesis pathway cloning, molecular characterization and phylogeny of lanosterol 14α-demethylase (ERG11) gene of Moniliophthora perniciosa |
| title_sort | analysis of the ergosterol biosynthesis pathway cloning molecular characterization and phylogeny of lanosterol 14α demethylase erg11 gene of moniliophthora perniciosa |
| topic | Basidiomycota fungus ergosterol Theobroma cacao |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500011&tlng=en |
| work_keys_str_mv | AT geruzadeoliveiraceita analysisoftheergosterolbiosynthesispathwaycloningmolecularcharacterizationandphylogenyoflanosterol14ademethylaseerg11geneofmoniliophthoraperniciosa AT laurivalantoniovilasboas analysisoftheergosterolbiosynthesispathwaycloningmolecularcharacterizationandphylogenyoflanosterol14ademethylaseerg11geneofmoniliophthoraperniciosa AT marcelosantoscastilho analysisoftheergosterolbiosynthesispathwaycloningmolecularcharacterizationandphylogenyoflanosterol14ademethylaseerg11geneofmoniliophthoraperniciosa AT marcelofalsarellacarazzolle analysisoftheergosterolbiosynthesispathwaycloningmolecularcharacterizationandphylogenyoflanosterol14ademethylaseerg11geneofmoniliophthoraperniciosa AT carlospriminhopirovani analysisoftheergosterolbiosynthesispathwaycloningmolecularcharacterizationandphylogenyoflanosterol14ademethylaseerg11geneofmoniliophthoraperniciosa AT alessandraselbachschnadelbach analysisoftheergosterolbiosynthesispathwaycloningmolecularcharacterizationandphylogenyoflanosterol14ademethylaseerg11geneofmoniliophthoraperniciosa AT karinaperesgramacho analysisoftheergosterolbiosynthesispathwaycloningmolecularcharacterizationandphylogenyoflanosterol14ademethylaseerg11geneofmoniliophthoraperniciosa AT pabloivanpereiraramos analysisoftheergosterolbiosynthesispathwaycloningmolecularcharacterizationandphylogenyoflanosterol14ademethylaseerg11geneofmoniliophthoraperniciosa AT lucianaveigabarbosa analysisoftheergosterolbiosynthesispathwaycloningmolecularcharacterizationandphylogenyoflanosterol14ademethylaseerg11geneofmoniliophthoraperniciosa AT goncaloamaranteguimaraespereira analysisoftheergosterolbiosynthesispathwaycloningmolecularcharacterizationandphylogenyoflanosterol14ademethylaseerg11geneofmoniliophthoraperniciosa AT aristotelesgoesneto analysisoftheergosterolbiosynthesispathwaycloningmolecularcharacterizationandphylogenyoflanosterol14ademethylaseerg11geneofmoniliophthoraperniciosa |