| Summary: | Although the crossover (CO) patterns of different species have been extensively investigated, little is known about the landscape of CO patterns in <i>Populus</i> because of its high heterozygosity and long-time generation. A novel strategy was proposed to reveal the difference of CO rate and interference between <i>Populus deltoides</i> and <i>Populus simonii</i> using their F1 hybrid population. We chose restriction site-associated DNA (RAD) tags that contained two SNPs, one only receiving the CO information from the female <i>P. deltoides</i> and the other from the male <i>P. simonii</i>. These RAD tags allowed us to investigate the CO patterns between the two outbred species, instead of using the traditional backcross populations in inbred lines. We found that the CO rate in <i>P. deltoides</i> was generally greater than that in <i>P. simonii</i>, and that the CO interference was a common phenomenon across the two genomes. The COs landscape of the different <i>Populus</i> species facilitates not only to understand the evolutionary mechanism for adaptability but also to rebuild the statistical model for precisely constructing genetic linkage maps that are critical in genome assembly in <i>Populus</i>. Additionally, the novel strategy could be applied in other outbred species for investigating the CO patterns.
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