ITS2 variability of Biomphalaria (Mollusca, Planorbidae) species from the Paranapanema Valley (São Paulo State, Brazil): diversity patterns, population structure, and phylogenetic relationships
The ribosomal DNA internal transcribed spacer 2 (ITS2) has been shown to be a useful genetic marker for species identification and phylogenetic reconstruction in the genus of freshwater snails Biomphalaria (Preston 1910). Additionally, ITS2 studies in Biomphalaria have uncovered significant intra-sp...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2007-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100024 |
| _version_ | 1818196137524330496 |
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| author | Roseli Tuan Priscila dos Santos |
| author_facet | Roseli Tuan Priscila dos Santos |
| author_sort | Roseli Tuan |
| collection | DOAJ |
| description | The ribosomal DNA internal transcribed spacer 2 (ITS2) has been shown to be a useful genetic marker for species identification and phylogenetic reconstruction in the genus of freshwater snails Biomphalaria (Preston 1910). Additionally, ITS2 studies in Biomphalaria have uncovered significant intra-specific genetic variability suggesting the presence of cryptic species complexes. We obtained ITS2 sequences for the Biomphalaria species B. glabrata, B. tenagophila, B. occidentalis and B. peregrina from the Paranapanema Valley (São Paulo State, Brazil) and compared them with a comprehensive set of published Biomphalaria ITS2 sequences using Bayesian inference of phylogeny. Analysis of the resulting trees showed that the newly obtained B. glabrata sequences did not cluster with those from other Brazilian localities and that sub-structuring occurred among Brazilian B. tenagophila populations. Moreover, although ITS2 sequences seem to indicate clear genetic differentiation within both B. glabrata and B. tenagophila, evidence in support of the occurrence of cryptic species is more compelling for the latter. We discuss the significance and implications of the detected patterns of ITS2 variability for taxonomic studies in Biomphalaria. |
| first_indexed | 2024-12-12T01:29:18Z |
| format | Article |
| id | doaj.art-62d9d1ba98b64762a576e9c78744d9fa |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-12T01:29:18Z |
| publishDate | 2007-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-62d9d1ba98b64762a576e9c78744d9fa2022-12-22T00:43:01ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852007-01-0130113914410.1590/S1415-47572007000100024ITS2 variability of Biomphalaria (Mollusca, Planorbidae) species from the Paranapanema Valley (São Paulo State, Brazil): diversity patterns, population structure, and phylogenetic relationshipsRoseli TuanPriscila dos SantosThe ribosomal DNA internal transcribed spacer 2 (ITS2) has been shown to be a useful genetic marker for species identification and phylogenetic reconstruction in the genus of freshwater snails Biomphalaria (Preston 1910). Additionally, ITS2 studies in Biomphalaria have uncovered significant intra-specific genetic variability suggesting the presence of cryptic species complexes. We obtained ITS2 sequences for the Biomphalaria species B. glabrata, B. tenagophila, B. occidentalis and B. peregrina from the Paranapanema Valley (São Paulo State, Brazil) and compared them with a comprehensive set of published Biomphalaria ITS2 sequences using Bayesian inference of phylogeny. Analysis of the resulting trees showed that the newly obtained B. glabrata sequences did not cluster with those from other Brazilian localities and that sub-structuring occurred among Brazilian B. tenagophila populations. Moreover, although ITS2 sequences seem to indicate clear genetic differentiation within both B. glabrata and B. tenagophila, evidence in support of the occurrence of cryptic species is more compelling for the latter. We discuss the significance and implications of the detected patterns of ITS2 variability for taxonomic studies in Biomphalaria.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100024Bayesian inferenceBiomphalariaDNA sequencesinternal transcribed spacer 2 (ITS2)ribosomal DNA |
| spellingShingle | Roseli Tuan Priscila dos Santos ITS2 variability of Biomphalaria (Mollusca, Planorbidae) species from the Paranapanema Valley (São Paulo State, Brazil): diversity patterns, population structure, and phylogenetic relationships Genetics and Molecular Biology Bayesian inference Biomphalaria DNA sequences internal transcribed spacer 2 (ITS2) ribosomal DNA |
| title | ITS2 variability of Biomphalaria (Mollusca, Planorbidae) species from the Paranapanema Valley (São Paulo State, Brazil): diversity patterns, population structure, and phylogenetic relationships |
| title_full | ITS2 variability of Biomphalaria (Mollusca, Planorbidae) species from the Paranapanema Valley (São Paulo State, Brazil): diversity patterns, population structure, and phylogenetic relationships |
| title_fullStr | ITS2 variability of Biomphalaria (Mollusca, Planorbidae) species from the Paranapanema Valley (São Paulo State, Brazil): diversity patterns, population structure, and phylogenetic relationships |
| title_full_unstemmed | ITS2 variability of Biomphalaria (Mollusca, Planorbidae) species from the Paranapanema Valley (São Paulo State, Brazil): diversity patterns, population structure, and phylogenetic relationships |
| title_short | ITS2 variability of Biomphalaria (Mollusca, Planorbidae) species from the Paranapanema Valley (São Paulo State, Brazil): diversity patterns, population structure, and phylogenetic relationships |
| title_sort | its2 variability of biomphalaria mollusca planorbidae species from the paranapanema valley sao paulo state brazil diversity patterns population structure and phylogenetic relationships |
| topic | Bayesian inference Biomphalaria DNA sequences internal transcribed spacer 2 (ITS2) ribosomal DNA |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100024 |
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