Separation of apolipoprotein B species by agarose-acrylamide gel electrophoresis.
Human apolipoprotein (apo) B has been recognized to exist in two different forms designated apoB-100 and apoB-48. The two apoB forms are usually separated by NaDodSO4 gel electrophoresis with a low percentage polyacrylamide gel in a tube gel apparatus. However, the matrix of this low percentage gel...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1988-06-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520388271 |
| _version_ | 1818862769194139648 |
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| author | C Gabelli D G Stark R E Gregg H B Brewer, Jr |
| author_facet | C Gabelli D G Stark R E Gregg H B Brewer, Jr |
| author_sort | C Gabelli |
| collection | DOAJ |
| description | Human apolipoprotein (apo) B has been recognized to exist in two different forms designated apoB-100 and apoB-48. The two apoB forms are usually separated by NaDodSO4 gel electrophoresis with a low percentage polyacrylamide gel in a tube gel apparatus. However, the matrix of this low percentage gel is relatively weak, and one can separate the two forms of apoB in a slab gel apparatus only if one utilizes a gradient polyacrylamide gel or a higher percentage polyacrylamide gel which results in a poorer separation of the protein bands. We have developed an agarose-acrylamide gel electrophoretic method to separate the two major apoB forms. The gel is a mixture of 0.5% agarose and 2% acrylamide. The agarose-acrylamide method is fast, has the advantage of being able to be used on an analytical or preparative scale in a vertical slab gel apparatus, and the gel is of sufficient strength to be used in immunoblotting and/or radioautography. |
| first_indexed | 2024-12-19T10:05:08Z |
| format | Article |
| id | doaj.art-62de019081594b4ba982849b386d18ef |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-19T10:05:08Z |
| publishDate | 1988-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-62de019081594b4ba982849b386d18ef2022-12-21T20:26:31ZengElsevierJournal of Lipid Research0022-22751988-06-01274457460Separation of apolipoprotein B species by agarose-acrylamide gel electrophoresis.C GabelliD G StarkR E GreggH B Brewer, JrHuman apolipoprotein (apo) B has been recognized to exist in two different forms designated apoB-100 and apoB-48. The two apoB forms are usually separated by NaDodSO4 gel electrophoresis with a low percentage polyacrylamide gel in a tube gel apparatus. However, the matrix of this low percentage gel is relatively weak, and one can separate the two forms of apoB in a slab gel apparatus only if one utilizes a gradient polyacrylamide gel or a higher percentage polyacrylamide gel which results in a poorer separation of the protein bands. We have developed an agarose-acrylamide gel electrophoretic method to separate the two major apoB forms. The gel is a mixture of 0.5% agarose and 2% acrylamide. The agarose-acrylamide method is fast, has the advantage of being able to be used on an analytical or preparative scale in a vertical slab gel apparatus, and the gel is of sufficient strength to be used in immunoblotting and/or radioautography.http://www.sciencedirect.com/science/article/pii/S0022227520388271 |
| spellingShingle | C Gabelli D G Stark R E Gregg H B Brewer, Jr Separation of apolipoprotein B species by agarose-acrylamide gel electrophoresis. Journal of Lipid Research |
| title | Separation of apolipoprotein B species by agarose-acrylamide gel electrophoresis. |
| title_full | Separation of apolipoprotein B species by agarose-acrylamide gel electrophoresis. |
| title_fullStr | Separation of apolipoprotein B species by agarose-acrylamide gel electrophoresis. |
| title_full_unstemmed | Separation of apolipoprotein B species by agarose-acrylamide gel electrophoresis. |
| title_short | Separation of apolipoprotein B species by agarose-acrylamide gel electrophoresis. |
| title_sort | separation of apolipoprotein b species by agarose acrylamide gel electrophoresis |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520388271 |
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