The Differential Expression and Snp Analysis of the Ovoinhibitor Gene in the Ovaries of Laying Duck Breeds (Anas Platyrhynchos)
ABSTRACT Ovoinhibitor (OIH) is the main proteinase inhibitor in the egg white. In the present study, real-time quantitative PCR and Western-Blot were used to analyze different expression pattern of OIH in ovaries as a candidate gene of reproductive traits in Jingjiang ducks (JJ ducks) and Shaoxing d...
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| Format: | Article |
| Language: | English |
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Fundação APINCO de Ciência e Tecnologia Avícolas
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| Series: | Brazilian Journal of Poultry Science |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000200281&lng=en&tlng=en |
| _version_ | 1818134371241033728 |
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| author | Y Wu H Liang H Zhang JS Pi AL Pan J Shen YJ Pu JP Du |
| author_facet | Y Wu H Liang H Zhang JS Pi AL Pan J Shen YJ Pu JP Du |
| author_sort | Y Wu |
| collection | DOAJ |
| description | ABSTRACT Ovoinhibitor (OIH) is the main proteinase inhibitor in the egg white. In the present study, real-time quantitative PCR and Western-Blot were used to analyze different expression pattern of OIH in ovaries as a candidate gene of reproductive traits in Jingjiang ducks (JJ ducks) and Shaoxing ducks (SX ducks) during three laying stages. To study the polymorphism of the OIH gene in those two duck populations, we designed five pairs of primers to detect SNPs of exon 3-5, 5-6, 14-16 and intron 7, 9 of the OIH gene by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA pool sequencing methods. The results showed that OIH expression increased during the laying stage in the ovaries of both duck breeds. The relative expression levels of OIH in the egg at hatch and 180days of age were lower in JJ ducks than in SX ducks, but higher in JJ ducks than SX ducks at 500 days of age. Only exon 5-6 locus had a novel SNP. One variation (389G>A) was detected in the two tested duck populations and it was associated with some laying traits, such as body weight of hatch, age at first egg, weight at first egg, egg number at 72weeks of age. The AG genotype was associated with inferior body weight of hatch and superior weight at first egg, age at first egg and egg number at 72weeks of age. Therefore, these results suggest that OIH may be a strong candidate gene related to some laying traits in ducks. |
| first_indexed | 2024-12-11T09:07:33Z |
| format | Article |
| id | doaj.art-62e0764120a2459f93aa97726f3d29bb |
| institution | Directory Open Access Journal |
| issn | 1806-9061 |
| language | English |
| last_indexed | 2024-12-11T09:07:33Z |
| publisher | Fundação APINCO de Ciência e Tecnologia Avícolas |
| record_format | Article |
| series | Brazilian Journal of Poultry Science |
| spelling | doaj.art-62e0764120a2459f93aa97726f3d29bb2022-12-22T01:13:35ZengFundação APINCO de Ciência e Tecnologia AvícolasBrazilian Journal of Poultry Science1806-906120228128610.1590/1806-9061-2017-0665S1516-635X2018000200281The Differential Expression and Snp Analysis of the Ovoinhibitor Gene in the Ovaries of Laying Duck Breeds (Anas Platyrhynchos)Y WuH LiangH ZhangJS PiAL PanJ ShenYJ PuJP DuABSTRACT Ovoinhibitor (OIH) is the main proteinase inhibitor in the egg white. In the present study, real-time quantitative PCR and Western-Blot were used to analyze different expression pattern of OIH in ovaries as a candidate gene of reproductive traits in Jingjiang ducks (JJ ducks) and Shaoxing ducks (SX ducks) during three laying stages. To study the polymorphism of the OIH gene in those two duck populations, we designed five pairs of primers to detect SNPs of exon 3-5, 5-6, 14-16 and intron 7, 9 of the OIH gene by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA pool sequencing methods. The results showed that OIH expression increased during the laying stage in the ovaries of both duck breeds. The relative expression levels of OIH in the egg at hatch and 180days of age were lower in JJ ducks than in SX ducks, but higher in JJ ducks than SX ducks at 500 days of age. Only exon 5-6 locus had a novel SNP. One variation (389G>A) was detected in the two tested duck populations and it was associated with some laying traits, such as body weight of hatch, age at first egg, weight at first egg, egg number at 72weeks of age. The AG genotype was associated with inferior body weight of hatch and superior weight at first egg, age at first egg and egg number at 72weeks of age. Therefore, these results suggest that OIH may be a strong candidate gene related to some laying traits in ducks.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000200281&lng=en&tlng=enLaying ducks (Anas platyrhynchos)laying periodgene expressionOIHovaries |
| spellingShingle | Y Wu H Liang H Zhang JS Pi AL Pan J Shen YJ Pu JP Du The Differential Expression and Snp Analysis of the Ovoinhibitor Gene in the Ovaries of Laying Duck Breeds (Anas Platyrhynchos) Brazilian Journal of Poultry Science Laying ducks (Anas platyrhynchos) laying period gene expression OIH ovaries |
| title | The Differential Expression and Snp Analysis of the Ovoinhibitor Gene in the Ovaries of Laying Duck Breeds (Anas Platyrhynchos) |
| title_full | The Differential Expression and Snp Analysis of the Ovoinhibitor Gene in the Ovaries of Laying Duck Breeds (Anas Platyrhynchos) |
| title_fullStr | The Differential Expression and Snp Analysis of the Ovoinhibitor Gene in the Ovaries of Laying Duck Breeds (Anas Platyrhynchos) |
| title_full_unstemmed | The Differential Expression and Snp Analysis of the Ovoinhibitor Gene in the Ovaries of Laying Duck Breeds (Anas Platyrhynchos) |
| title_short | The Differential Expression and Snp Analysis of the Ovoinhibitor Gene in the Ovaries of Laying Duck Breeds (Anas Platyrhynchos) |
| title_sort | differential expression and snp analysis of the ovoinhibitor gene in the ovaries of laying duck breeds anas platyrhynchos |
| topic | Laying ducks (Anas platyrhynchos) laying period gene expression OIH ovaries |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000200281&lng=en&tlng=en |
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