Isolation, characterization, development and evaluation of phytoconstituent based formulation for diabetic neuropathy
Background: Morus alba Linn, referred to as white mulberry, is a potential traditional medicine for diabetes and neuroprotection. Aim: Isolation, characterization, development and evaluation of phytoconstituent based formulation for diabetic neuropathy. Material and methods: The stem Bark of M. alba...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Saudi Pharmaceutical Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319016423001792 |
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| author | Rashmi Pathak Neetu Sachan Atul Kabra Ashwag S. Alanazi Mohammed M. Alanazi Nawaf A. Alsaif Phool Chandra |
| author_facet | Rashmi Pathak Neetu Sachan Atul Kabra Ashwag S. Alanazi Mohammed M. Alanazi Nawaf A. Alsaif Phool Chandra |
| author_sort | Rashmi Pathak |
| collection | DOAJ |
| description | Background: Morus alba Linn, referred to as white mulberry, is a potential traditional medicine for diabetes and neuroprotection. Aim: Isolation, characterization, development and evaluation of phytoconstituent based formulation for diabetic neuropathy. Material and methods: The stem Bark of M. alba was peeled and subjected to extraction. A phytoconstituent was then isolated by column chromatography and characterized using Mass spectroscopy, FTIR, and NMR. The isolated phytoconstituent was used to formulate a nanoemulsion. Nanoemulsion was also characterized for viscosity, surface tension, refractive index, pH, and particle size. Selected nanoemulsion formulations were then tested for acute oral toxicity and diabetic neuropathy, including behavioral, hematological, histopathological, and biomarker examinations. Results: The spectral analysis affirmed that the isolated compound was found to be chrysin. A nanoemulsion formulation was made using the chrysin and was characterized and found to be stable during the stability testing and fulfilled all other testing parameters. Then acute oral toxicity study of the formulations was found to be safe. Formulations were found to possess significant results against diabetic neuropathy in rats. Biomarkers were analyzed for their mechanistic involvement in reducing neuropathy in rats, and it was found that the oxidative pathway was considerably restored, suggesting that chrysin causes these effects via this pathway. Conclusions: Results suggests that isolated phytoconstituent (chrysin) from the bark of Morus alba derived nanoemulsion has protective and beneficial effects by diminishing the oxidative damage against alloxan-induced diabetic neuropathy in rats. |
| first_indexed | 2024-03-12T17:27:27Z |
| format | Article |
| id | doaj.art-62e1c830cf464ce9ae36f116092f64be |
| institution | Directory Open Access Journal |
| issn | 1319-0164 |
| language | English |
| last_indexed | 2024-03-12T17:27:27Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Pharmaceutical Journal |
| spelling | doaj.art-62e1c830cf464ce9ae36f116092f64be2023-08-05T05:15:17ZengElsevierSaudi Pharmaceutical Journal1319-01642023-08-01318101687Isolation, characterization, development and evaluation of phytoconstituent based formulation for diabetic neuropathyRashmi Pathak0Neetu Sachan1Atul Kabra2Ashwag S. Alanazi3Mohammed M. Alanazi4Nawaf A. Alsaif5Phool Chandra6Department of Pharmacy, Invertis University, Bareilly-243123, Uttar Pradesh, IndiaMaharana Pratap College of Pharmacy, Mandhana, Kanpur-209217, Uttar Pradesh, IndiaUniversity Institute of Pharma Sciences, Chandigarh University, Gharuan, 140301 Mohali, Punjab, IndiaDepartment of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh aadea, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh aadea, Saudi ArabiaTeerthanker Mahaveer College of Pharmacy, Teerthanker Mahaveer University, Moradabad-244001, Uttar Pradesh, India; Corresponding author.Background: Morus alba Linn, referred to as white mulberry, is a potential traditional medicine for diabetes and neuroprotection. Aim: Isolation, characterization, development and evaluation of phytoconstituent based formulation for diabetic neuropathy. Material and methods: The stem Bark of M. alba was peeled and subjected to extraction. A phytoconstituent was then isolated by column chromatography and characterized using Mass spectroscopy, FTIR, and NMR. The isolated phytoconstituent was used to formulate a nanoemulsion. Nanoemulsion was also characterized for viscosity, surface tension, refractive index, pH, and particle size. Selected nanoemulsion formulations were then tested for acute oral toxicity and diabetic neuropathy, including behavioral, hematological, histopathological, and biomarker examinations. Results: The spectral analysis affirmed that the isolated compound was found to be chrysin. A nanoemulsion formulation was made using the chrysin and was characterized and found to be stable during the stability testing and fulfilled all other testing parameters. Then acute oral toxicity study of the formulations was found to be safe. Formulations were found to possess significant results against diabetic neuropathy in rats. Biomarkers were analyzed for their mechanistic involvement in reducing neuropathy in rats, and it was found that the oxidative pathway was considerably restored, suggesting that chrysin causes these effects via this pathway. Conclusions: Results suggests that isolated phytoconstituent (chrysin) from the bark of Morus alba derived nanoemulsion has protective and beneficial effects by diminishing the oxidative damage against alloxan-induced diabetic neuropathy in rats.http://www.sciencedirect.com/science/article/pii/S1319016423001792Morus albaIsolationChrysinNanoemulsionDiabetic Neuropathy |
| spellingShingle | Rashmi Pathak Neetu Sachan Atul Kabra Ashwag S. Alanazi Mohammed M. Alanazi Nawaf A. Alsaif Phool Chandra Isolation, characterization, development and evaluation of phytoconstituent based formulation for diabetic neuropathy Saudi Pharmaceutical Journal Morus alba Isolation Chrysin Nanoemulsion Diabetic Neuropathy |
| title | Isolation, characterization, development and evaluation of phytoconstituent based formulation for diabetic neuropathy |
| title_full | Isolation, characterization, development and evaluation of phytoconstituent based formulation for diabetic neuropathy |
| title_fullStr | Isolation, characterization, development and evaluation of phytoconstituent based formulation for diabetic neuropathy |
| title_full_unstemmed | Isolation, characterization, development and evaluation of phytoconstituent based formulation for diabetic neuropathy |
| title_short | Isolation, characterization, development and evaluation of phytoconstituent based formulation for diabetic neuropathy |
| title_sort | isolation characterization development and evaluation of phytoconstituent based formulation for diabetic neuropathy |
| topic | Morus alba Isolation Chrysin Nanoemulsion Diabetic Neuropathy |
| url | http://www.sciencedirect.com/science/article/pii/S1319016423001792 |
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