Prioritization of Microorganisms Isolated from the Indian Ocean Sponge <i>Scopalina hapalia</i> Based on Metabolomic Diversity and Biological Activity for the Discovery of Natural Products
Despite considerable advances in medicine and technology, humanity still faces many deadly diseases such as cancer and malaria. In order to find appropriate treatments, the discovery of new bioactive substances is essential. Therefore, research is now turning to less frequently explored habitats wit...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/3/697 |
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| author | Alexandre Le Loarer Rémy Marcellin-Gros Laurent Dufossé Jérôme Bignon Michel Frédérich Allison Ledoux Emerson Ferreira Queiroz Jean-Luc Wolfender Anne Gauvin-Bialecki Mireille Fouillaud |
| author_facet | Alexandre Le Loarer Rémy Marcellin-Gros Laurent Dufossé Jérôme Bignon Michel Frédérich Allison Ledoux Emerson Ferreira Queiroz Jean-Luc Wolfender Anne Gauvin-Bialecki Mireille Fouillaud |
| author_sort | Alexandre Le Loarer |
| collection | DOAJ |
| description | Despite considerable advances in medicine and technology, humanity still faces many deadly diseases such as cancer and malaria. In order to find appropriate treatments, the discovery of new bioactive substances is essential. Therefore, research is now turning to less frequently explored habitats with exceptional biodiversity such as the marine environment. Many studies have demonstrated the therapeutic potential of bioactive compounds from marine macro- and microorganisms. In this study, nine microbial strains isolated from an Indian Ocean sponge, <i>Scopalina hapalia,</i> were screened for their chemical potential. The isolates belong to different phyla, some of which are already known for their production of secondary metabolites, such as the actinobacteria. This article aims at describing the selection method used to identify the most promising microorganisms in the field of active metabolites production. The method is based on the combination of their biological and chemical screening, coupled with the use of bioinformatic tools. The dereplication of microbial extracts and the creation of a molecular network revealed the presence of known bioactive molecules such as staurosporin, erythromycin and chaetoglobosins. Molecular network exploration indicated the possible presence of novel compounds in clusters of interest. The biological activities targeted in the study were cytotoxicity against the HCT-116 and MDA-MB-231 cell lines and antiplasmodial activity against <i>Plasmodium falciparum</i> 3D7. <i>Chaetomium globosum</i> SH-123 and <i>Salinispora arenicola</i> SH-78 strains actually showed remarkable cytotoxic and antiplasmodial activities, while <i>Micromonospora fluostatini</i> SH-82 demonstrated promising antiplasmodial effects. The ranking of the microorganisms as a result of the different screening steps allowed the selection of a promising strain, <i>Micromonospora fluostatini</i> SH-82, as a premium candidate for the discovery of new drugs. |
| first_indexed | 2024-03-09T10:59:10Z |
| format | Article |
| id | doaj.art-62e39b9558274aefbb561f7383a7a4cc |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T10:59:10Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-62e39b9558274aefbb561f7383a7a4cc2023-12-01T01:23:04ZengMDPI AGMicroorganisms2076-26072023-03-0111369710.3390/microorganisms11030697Prioritization of Microorganisms Isolated from the Indian Ocean Sponge <i>Scopalina hapalia</i> Based on Metabolomic Diversity and Biological Activity for the Discovery of Natural ProductsAlexandre Le Loarer0Rémy Marcellin-Gros1Laurent Dufossé2Jérôme Bignon3Michel Frédérich4Allison Ledoux5Emerson Ferreira Queiroz6Jean-Luc Wolfender7Anne Gauvin-Bialecki8Mireille Fouillaud9Laboratory of Chemistry and Biotechnology of Natural Products, Faculty of Science and Technology, University of La Réunion, 15 Avenue René Cassin, CS 92003, CEDEX 09, 97744 Saint-Denis, FranceInstitute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, CMU-Rue Michel-Servet 1, CH-1211 Geneva, SwitzerlandLaboratory of Chemistry and Biotechnology of Natural Products, Faculty of Science and Technology, University of La Réunion, 15 Avenue René Cassin, CS 92003, CEDEX 09, 97744 Saint-Denis, FranceInstitute of Chemistry of Natural Substances (ICSN), CNRS UPR 2301, Université Paris-Saclay, 1, av. de la Terrasse, CEDEX, 91198 Gif-sur-Yvette, FrancePharmacognosy Laboratory, Department of Pharmacy, Centre Interfacultaire de Recherche sur le Médicament (CIRM), University of Liège, Quartier Hôpital, Avenue Hippocrate 15, Bat. B36, Campus du Sart-Tilman, B-4000 Liege, BelgiumPharmacognosy Laboratory, Department of Pharmacy, Centre Interfacultaire de Recherche sur le Médicament (CIRM), University of Liège, Quartier Hôpital, Avenue Hippocrate 15, Bat. B36, Campus du Sart-Tilman, B-4000 Liege, BelgiumInstitute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, CMU-Rue Michel-Servet 1, CH-1211 Geneva, SwitzerlandInstitute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, CMU-Rue Michel-Servet 1, CH-1211 Geneva, SwitzerlandLaboratory of Chemistry and Biotechnology of Natural Products, Faculty of Science and Technology, University of La Réunion, 15 Avenue René Cassin, CS 92003, CEDEX 09, 97744 Saint-Denis, FranceLaboratory of Chemistry and Biotechnology of Natural Products, Faculty of Science and Technology, University of La Réunion, 15 Avenue René Cassin, CS 92003, CEDEX 09, 97744 Saint-Denis, FranceDespite considerable advances in medicine and technology, humanity still faces many deadly diseases such as cancer and malaria. In order to find appropriate treatments, the discovery of new bioactive substances is essential. Therefore, research is now turning to less frequently explored habitats with exceptional biodiversity such as the marine environment. Many studies have demonstrated the therapeutic potential of bioactive compounds from marine macro- and microorganisms. In this study, nine microbial strains isolated from an Indian Ocean sponge, <i>Scopalina hapalia,</i> were screened for their chemical potential. The isolates belong to different phyla, some of which are already known for their production of secondary metabolites, such as the actinobacteria. This article aims at describing the selection method used to identify the most promising microorganisms in the field of active metabolites production. The method is based on the combination of their biological and chemical screening, coupled with the use of bioinformatic tools. The dereplication of microbial extracts and the creation of a molecular network revealed the presence of known bioactive molecules such as staurosporin, erythromycin and chaetoglobosins. Molecular network exploration indicated the possible presence of novel compounds in clusters of interest. The biological activities targeted in the study were cytotoxicity against the HCT-116 and MDA-MB-231 cell lines and antiplasmodial activity against <i>Plasmodium falciparum</i> 3D7. <i>Chaetomium globosum</i> SH-123 and <i>Salinispora arenicola</i> SH-78 strains actually showed remarkable cytotoxic and antiplasmodial activities, while <i>Micromonospora fluostatini</i> SH-82 demonstrated promising antiplasmodial effects. The ranking of the microorganisms as a result of the different screening steps allowed the selection of a promising strain, <i>Micromonospora fluostatini</i> SH-82, as a premium candidate for the discovery of new drugs.https://www.mdpi.com/2076-2607/11/3/697natural productsmarine microorganismsspecialized metabolitesmolecular networkdereplicationcytotoxic activity |
| spellingShingle | Alexandre Le Loarer Rémy Marcellin-Gros Laurent Dufossé Jérôme Bignon Michel Frédérich Allison Ledoux Emerson Ferreira Queiroz Jean-Luc Wolfender Anne Gauvin-Bialecki Mireille Fouillaud Prioritization of Microorganisms Isolated from the Indian Ocean Sponge <i>Scopalina hapalia</i> Based on Metabolomic Diversity and Biological Activity for the Discovery of Natural Products Microorganisms natural products marine microorganisms specialized metabolites molecular network dereplication cytotoxic activity |
| title | Prioritization of Microorganisms Isolated from the Indian Ocean Sponge <i>Scopalina hapalia</i> Based on Metabolomic Diversity and Biological Activity for the Discovery of Natural Products |
| title_full | Prioritization of Microorganisms Isolated from the Indian Ocean Sponge <i>Scopalina hapalia</i> Based on Metabolomic Diversity and Biological Activity for the Discovery of Natural Products |
| title_fullStr | Prioritization of Microorganisms Isolated from the Indian Ocean Sponge <i>Scopalina hapalia</i> Based on Metabolomic Diversity and Biological Activity for the Discovery of Natural Products |
| title_full_unstemmed | Prioritization of Microorganisms Isolated from the Indian Ocean Sponge <i>Scopalina hapalia</i> Based on Metabolomic Diversity and Biological Activity for the Discovery of Natural Products |
| title_short | Prioritization of Microorganisms Isolated from the Indian Ocean Sponge <i>Scopalina hapalia</i> Based on Metabolomic Diversity and Biological Activity for the Discovery of Natural Products |
| title_sort | prioritization of microorganisms isolated from the indian ocean sponge i scopalina hapalia i based on metabolomic diversity and biological activity for the discovery of natural products |
| topic | natural products marine microorganisms specialized metabolites molecular network dereplication cytotoxic activity |
| url | https://www.mdpi.com/2076-2607/11/3/697 |
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