Genetic responses of the marine copepod Acartia tonsa (Dana) to heat shock and epibiont infestation
Expression of stress-related genes was investigated in the marine copepod Acartia tonsa in relation to heat shock at two different salinities (10 and 32‰), and it was furthermore investigated whether experimentally induced epibiont infestation led to elevated expression of stress-related genes. Expr...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-11-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513415000113 |
| _version_ | 1818481196612452352 |
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| author | Egle Petkeviciute Per W. Kania Alf Skovgaard |
| author_facet | Egle Petkeviciute Per W. Kania Alf Skovgaard |
| author_sort | Egle Petkeviciute |
| collection | DOAJ |
| description | Expression of stress-related genes was investigated in the marine copepod Acartia tonsa in relation to heat shock at two different salinities (10 and 32‰), and it was furthermore investigated whether experimentally induced epibiont infestation led to elevated expression of stress-related genes. Expression of the genes ferritin, Hsp90 and Hsp70 were analyzed in adult copepods by conducting reverse transcription-quantitative real time PCR (RT-qPCR). The expression of Hsp70 and Hsp90 was significantly up-regulated after heat shock and the expression levels were higher in copepods cultivated at 10‰ salinity seawater than in copepods cultivated at 32‰. Significant up-regulation of ferritin (3.3 fold increase) was observed as a response to infestation with the epibiotic euglenid Colacium vesiculosum. Results suggest that (i) A. tonsa responds more pronounced to thermal shock when cultivated in low salinity seawater (10‰) as compared to optimal salinity seawater (32‰) and (ii) epibiont infestation does cause a measureable physiological response in the host. |
| first_indexed | 2024-12-10T11:31:45Z |
| format | Article |
| id | doaj.art-62e4f30ea18949f788495f399e963304 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-12-10T11:31:45Z |
| publishDate | 2015-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-62e4f30ea18949f788495f399e9633042022-12-22T01:50:34ZengElsevierAquaculture Reports2352-51342015-11-012C101610.1016/j.aqrep.2015.04.001Genetic responses of the marine copepod Acartia tonsa (Dana) to heat shock and epibiont infestationEgle Petkeviciute0Per W. Kania1Alf Skovgaard2Laboratory of Aquatic Pathobiology, Department of Veterinary Disease Biology, University of Copenhagen, 1870 Frederiksberg, DenmarkLaboratory of Aquatic Pathobiology, Department of Veterinary Disease Biology, University of Copenhagen, 1870 Frederiksberg, DenmarkLaboratory of Aquatic Pathobiology, Department of Veterinary Disease Biology, University of Copenhagen, 1870 Frederiksberg, DenmarkExpression of stress-related genes was investigated in the marine copepod Acartia tonsa in relation to heat shock at two different salinities (10 and 32‰), and it was furthermore investigated whether experimentally induced epibiont infestation led to elevated expression of stress-related genes. Expression of the genes ferritin, Hsp90 and Hsp70 were analyzed in adult copepods by conducting reverse transcription-quantitative real time PCR (RT-qPCR). The expression of Hsp70 and Hsp90 was significantly up-regulated after heat shock and the expression levels were higher in copepods cultivated at 10‰ salinity seawater than in copepods cultivated at 32‰. Significant up-regulation of ferritin (3.3 fold increase) was observed as a response to infestation with the epibiotic euglenid Colacium vesiculosum. Results suggest that (i) A. tonsa responds more pronounced to thermal shock when cultivated in low salinity seawater (10‰) as compared to optimal salinity seawater (32‰) and (ii) epibiont infestation does cause a measureable physiological response in the host.http://www.sciencedirect.com/science/article/pii/S2352513415000113Acartia tonsaβ-ActinEfa-1αEpibiont infestationFerritinHeat shockHsp70Hsp90Gene expression |
| spellingShingle | Egle Petkeviciute Per W. Kania Alf Skovgaard Genetic responses of the marine copepod Acartia tonsa (Dana) to heat shock and epibiont infestation Aquaculture Reports Acartia tonsa β-Actin Efa-1α Epibiont infestation Ferritin Heat shock Hsp70 Hsp90 Gene expression |
| title | Genetic responses of the marine copepod Acartia tonsa (Dana) to heat shock and epibiont infestation |
| title_full | Genetic responses of the marine copepod Acartia tonsa (Dana) to heat shock and epibiont infestation |
| title_fullStr | Genetic responses of the marine copepod Acartia tonsa (Dana) to heat shock and epibiont infestation |
| title_full_unstemmed | Genetic responses of the marine copepod Acartia tonsa (Dana) to heat shock and epibiont infestation |
| title_short | Genetic responses of the marine copepod Acartia tonsa (Dana) to heat shock and epibiont infestation |
| title_sort | genetic responses of the marine copepod acartia tonsa dana to heat shock and epibiont infestation |
| topic | Acartia tonsa β-Actin Efa-1α Epibiont infestation Ferritin Heat shock Hsp70 Hsp90 Gene expression |
| url | http://www.sciencedirect.com/science/article/pii/S2352513415000113 |
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