Prevalence and molecular characterisation of Shiga toxin-producing Escherichia coli in raw milk cheeses from Lazio region, Italy
In recent years, the incidence of foodborne diseases caused by Shiga toxin-producing <em>Escherichia coli</em> (STEC) has increased globally. For this reason, within the specific regional control plan for the detection of STEC in food products in Italy, the presence of STEC in unpasteuri...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2016-02-01
|
| Series: | Italian Journal of Food Safety |
| Subjects: | |
| Online Access: | http://www.pagepressjournals.org/index.php/ijfs/article/view/4566 |
| _version_ | 1818211650047574016 |
|---|---|
| author | Selene Marozzi Paola De Santis Sarah Lovari Roberto Condoleo Stefano Bilei Rita Marcianò Ziad Mezher |
| author_facet | Selene Marozzi Paola De Santis Sarah Lovari Roberto Condoleo Stefano Bilei Rita Marcianò Ziad Mezher |
| author_sort | Selene Marozzi |
| collection | DOAJ |
| description | In recent years, the incidence of foodborne diseases caused by Shiga toxin-producing <em>Escherichia coli</em> (STEC) has increased globally. For this reason, within the specific regional control plan for the detection of STEC in food products in Italy, the presence of STEC in unpasteurised milk cheeses was investigated. In total, 203 samples obtained from March 2011 to December 2013 were analysed, with two standard methods (ISO 16654:2001 and ISO 13136:2012). Two strains of E. coli O157 were isolated (2/161, 1.2%) but did not carry any virulence-associated genes and 22 <em>stx</em>-positive samples (22/146, 15.1%) were detected in enrichment cultures, mostly from ovine cheeses. Only two strains isolated from different ovine cheeses carried <em>stx</em> gene and none of these was <em>eae</em>-positive. This study confirms the presence of <em>stx</em>-positive <em>E. coli</em> and suggests that this type of food cannot be excluded as a potential vehicle of STEC. |
| first_indexed | 2024-12-12T05:35:52Z |
| format | Article |
| id | doaj.art-62e56804713e458f856cb6efdc7c86df |
| institution | Directory Open Access Journal |
| issn | 2239-7132 |
| language | English |
| last_indexed | 2024-12-12T05:35:52Z |
| publishDate | 2016-02-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Italian Journal of Food Safety |
| spelling | doaj.art-62e56804713e458f856cb6efdc7c86df2022-12-22T00:36:08ZengPAGEPress PublicationsItalian Journal of Food Safety2239-71322016-02-015110.4081/ijfs.2016.45664638Prevalence and molecular characterisation of Shiga toxin-producing Escherichia coli in raw milk cheeses from Lazio region, ItalySelene Marozzi0Paola De Santis1Sarah Lovari2Roberto Condoleo3Stefano Bilei4Rita Marcianò5Ziad Mezher6Institute for Experimental Veterinary Medicine of Lazio and Tuscany, RomeInstitute for Experimental Veterinary Medicine of Lazio and Tuscany, RomeInstitute for Experimental Veterinary Medicine of Lazio and Tuscany, RomeInstitute for Experimental Veterinary Medicine of Lazio and Tuscany, RomeInstitute for Experimental Veterinary Medicine of Lazio and Tuscany, RomeLazio Region, Veterinary Health Section, RomeInstitute for Experimental Veterinary Medicine of Lazio and Tuscany, RomeIn recent years, the incidence of foodborne diseases caused by Shiga toxin-producing <em>Escherichia coli</em> (STEC) has increased globally. For this reason, within the specific regional control plan for the detection of STEC in food products in Italy, the presence of STEC in unpasteurised milk cheeses was investigated. In total, 203 samples obtained from March 2011 to December 2013 were analysed, with two standard methods (ISO 16654:2001 and ISO 13136:2012). Two strains of E. coli O157 were isolated (2/161, 1.2%) but did not carry any virulence-associated genes and 22 <em>stx</em>-positive samples (22/146, 15.1%) were detected in enrichment cultures, mostly from ovine cheeses. Only two strains isolated from different ovine cheeses carried <em>stx</em> gene and none of these was <em>eae</em>-positive. This study confirms the presence of <em>stx</em>-positive <em>E. coli</em> and suggests that this type of food cannot be excluded as a potential vehicle of STEC.http://www.pagepressjournals.org/index.php/ijfs/article/view/4566Shiga-toxin-producing Escherichia coliRaw milk cheesesPrevalence |
| spellingShingle | Selene Marozzi Paola De Santis Sarah Lovari Roberto Condoleo Stefano Bilei Rita Marcianò Ziad Mezher Prevalence and molecular characterisation of Shiga toxin-producing Escherichia coli in raw milk cheeses from Lazio region, Italy Italian Journal of Food Safety Shiga-toxin-producing Escherichia coli Raw milk cheeses Prevalence |
| title | Prevalence and molecular characterisation of Shiga toxin-producing Escherichia coli in raw milk cheeses from Lazio region, Italy |
| title_full | Prevalence and molecular characterisation of Shiga toxin-producing Escherichia coli in raw milk cheeses from Lazio region, Italy |
| title_fullStr | Prevalence and molecular characterisation of Shiga toxin-producing Escherichia coli in raw milk cheeses from Lazio region, Italy |
| title_full_unstemmed | Prevalence and molecular characterisation of Shiga toxin-producing Escherichia coli in raw milk cheeses from Lazio region, Italy |
| title_short | Prevalence and molecular characterisation of Shiga toxin-producing Escherichia coli in raw milk cheeses from Lazio region, Italy |
| title_sort | prevalence and molecular characterisation of shiga toxin producing escherichia coli in raw milk cheeses from lazio region italy |
| topic | Shiga-toxin-producing Escherichia coli Raw milk cheeses Prevalence |
| url | http://www.pagepressjournals.org/index.php/ijfs/article/view/4566 |
| work_keys_str_mv | AT selenemarozzi prevalenceandmolecularcharacterisationofshigatoxinproducingescherichiacoliinrawmilkcheesesfromlazioregionitaly AT paoladesantis prevalenceandmolecularcharacterisationofshigatoxinproducingescherichiacoliinrawmilkcheesesfromlazioregionitaly AT sarahlovari prevalenceandmolecularcharacterisationofshigatoxinproducingescherichiacoliinrawmilkcheesesfromlazioregionitaly AT robertocondoleo prevalenceandmolecularcharacterisationofshigatoxinproducingescherichiacoliinrawmilkcheesesfromlazioregionitaly AT stefanobilei prevalenceandmolecularcharacterisationofshigatoxinproducingescherichiacoliinrawmilkcheesesfromlazioregionitaly AT ritamarciano prevalenceandmolecularcharacterisationofshigatoxinproducingescherichiacoliinrawmilkcheesesfromlazioregionitaly AT ziadmezher prevalenceandmolecularcharacterisationofshigatoxinproducingescherichiacoliinrawmilkcheesesfromlazioregionitaly |