Using synthetic peptides to benchmark peptide identification software and search parameters for MS/MS data analysis

Using synthetic peptides to benchmark peptide identification software and search parameters for MS/MS data analysis

Tandem mass spectrometry and sequence database searching are widely used in proteomics to identify peptides in complex mixtures. Here we present a benchmark study in which a pool of 20,103 synthetic peptides was measured and the resulting data set was analyzed using around 1800 different software an...

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Bibliographic Details
Main Authors: Andreas Quandt, Lucia Espona, Akos Balasko, Hendrik Weisser, Mi-Youn Brusniak, Peter Kunszt, Ruedi Aebersold, Lars Malmström
Format: Article
Language:English
Published: Elsevier 2014-12-01
Series:EuPA Open Proteomics
Subjects:
Mass spectrometry
Data analysis
Classical database search
Synthetic peptides
Search engine
Online Access:http://www.sciencedirect.com/science/article/pii/S221296851400066X

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