Protein Network Studies on PCOS Biomarkers With S100A8, Druggability Assessment, and RNA Aptamer Designing to Control Its Cyst Migration Effect
The prevalence of polycystic ovary syndrome (PCOS) has been gradually increasing among adult females worldwide. Laparoscopy drilling on ovary is the only available temporary solution with a high incidence of reoccurrence. S100A8 with S100A9 complex is believed to facilitate the cyst migration in PCO...
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| Format: | Article |
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Frontiers Media S.A.
2020-05-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2020.00328/full |
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| author | Subramaniyan Manibalan Ayyachamy Shobana Manickam Kiruthika Anant Achary Madasamy Swathi Renganathan Venkatalakshmi Kandasamy Thirukumaran K. Suhasini Sharon Roopathy |
| author_facet | Subramaniyan Manibalan Ayyachamy Shobana Manickam Kiruthika Anant Achary Madasamy Swathi Renganathan Venkatalakshmi Kandasamy Thirukumaran K. Suhasini Sharon Roopathy |
| author_sort | Subramaniyan Manibalan |
| collection | DOAJ |
| description | The prevalence of polycystic ovary syndrome (PCOS) has been gradually increasing among adult females worldwide. Laparoscopy drilling on ovary is the only available temporary solution with a high incidence of reoccurrence. S100A8 with S100A9 complex is believed to facilitate the cyst migration in PCOS condition. The high evident protein interaction network studies between PCOS biomarkers, cancer invasion markers, and the interactors of S100A8 confirm that this protein has strong interaction with other selective PCOS biomarkers, which may be associative in the immature cyst invasion process. Through the network studies, intensive structural and pathway analysis, S100A8 is identified as a targetable protein. In this research, the non-SELEX in silico method is adapted to construct RNA Library based on the consensus DNA sequence of Glucocorticoid Response Element (GRE) and screened the best nucleotide fragments which are bound within the active sites of the target protein. Selected sequences are joined as a single strand and screened the one which competitively binds with minimal energy. In vitro follow-up of this computational research, the designed RNA aptamer was used to infect the MCF7 cell line through Lipofectamine 2000 mediated delivery to study the anti-cell migration effect. Wound Scratch assay confirms that the synthesized 18-mer oligo has significant inhibition activity toward tumor cell migration at the cellular level. |
| first_indexed | 2024-12-14T03:53:52Z |
| format | Article |
| id | doaj.art-62e5e3b0470148bba7943030ae6a3016 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-14T03:53:52Z |
| publishDate | 2020-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-62e5e3b0470148bba7943030ae6a30162022-12-21T23:18:08ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-05-01810.3389/fbioe.2020.00328502690Protein Network Studies on PCOS Biomarkers With S100A8, Druggability Assessment, and RNA Aptamer Designing to Control Its Cyst Migration EffectSubramaniyan Manibalan0Ayyachamy Shobana1Manickam Kiruthika2Anant Achary3Madasamy Swathi4Renganathan Venkatalakshmi5Kandasamy Thirukumaran6K. Suhasini7Sharon Roopathy8Centre for Research, Kamaraj College of Engineering and Technology, Madurai, IndiaCentre for Research, Kamaraj College of Engineering and Technology, Madurai, IndiaCentre for Research, Kamaraj College of Engineering and Technology, Madurai, IndiaCentre for Research, Kamaraj College of Engineering and Technology, Madurai, IndiaCentre for Research, Kamaraj College of Engineering and Technology, Madurai, IndiaDepartment of Biotechnology, Kamaraj College of Engineering and Technology, Madurai, IndiaDepartment of Biotechnology, Kamaraj College of Engineering and Technology, Madurai, IndiaDepartment of Biotechnology, Kamaraj College of Engineering and Technology, Madurai, IndiaDepartment of Biotechnology, Kamaraj College of Engineering and Technology, Madurai, IndiaThe prevalence of polycystic ovary syndrome (PCOS) has been gradually increasing among adult females worldwide. Laparoscopy drilling on ovary is the only available temporary solution with a high incidence of reoccurrence. S100A8 with S100A9 complex is believed to facilitate the cyst migration in PCOS condition. The high evident protein interaction network studies between PCOS biomarkers, cancer invasion markers, and the interactors of S100A8 confirm that this protein has strong interaction with other selective PCOS biomarkers, which may be associative in the immature cyst invasion process. Through the network studies, intensive structural and pathway analysis, S100A8 is identified as a targetable protein. In this research, the non-SELEX in silico method is adapted to construct RNA Library based on the consensus DNA sequence of Glucocorticoid Response Element (GRE) and screened the best nucleotide fragments which are bound within the active sites of the target protein. Selected sequences are joined as a single strand and screened the one which competitively binds with minimal energy. In vitro follow-up of this computational research, the designed RNA aptamer was used to infect the MCF7 cell line through Lipofectamine 2000 mediated delivery to study the anti-cell migration effect. Wound Scratch assay confirms that the synthesized 18-mer oligo has significant inhibition activity toward tumor cell migration at the cellular level.https://www.frontiersin.org/article/10.3389/fbioe.2020.00328/fullnetwork analysisdruggabilityRNA aptamerlim methodpcos targetsprotein network |
| spellingShingle | Subramaniyan Manibalan Ayyachamy Shobana Manickam Kiruthika Anant Achary Madasamy Swathi Renganathan Venkatalakshmi Kandasamy Thirukumaran K. Suhasini Sharon Roopathy Protein Network Studies on PCOS Biomarkers With S100A8, Druggability Assessment, and RNA Aptamer Designing to Control Its Cyst Migration Effect Frontiers in Bioengineering and Biotechnology network analysis druggability RNA aptamer lim method pcos targets protein network |
| title | Protein Network Studies on PCOS Biomarkers With S100A8, Druggability Assessment, and RNA Aptamer Designing to Control Its Cyst Migration Effect |
| title_full | Protein Network Studies on PCOS Biomarkers With S100A8, Druggability Assessment, and RNA Aptamer Designing to Control Its Cyst Migration Effect |
| title_fullStr | Protein Network Studies on PCOS Biomarkers With S100A8, Druggability Assessment, and RNA Aptamer Designing to Control Its Cyst Migration Effect |
| title_full_unstemmed | Protein Network Studies on PCOS Biomarkers With S100A8, Druggability Assessment, and RNA Aptamer Designing to Control Its Cyst Migration Effect |
| title_short | Protein Network Studies on PCOS Biomarkers With S100A8, Druggability Assessment, and RNA Aptamer Designing to Control Its Cyst Migration Effect |
| title_sort | protein network studies on pcos biomarkers with s100a8 druggability assessment and rna aptamer designing to control its cyst migration effect |
| topic | network analysis druggability RNA aptamer lim method pcos targets protein network |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2020.00328/full |
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