Antibacterial Activity and Untargeted Metabolomics Profiling of <i>Acalypha arvensis</i> Poepp
The search for potent antimicrobial compounds is critical in the face of growing antibiotic resistance. This study explores <i>Acalypha arvensis</i> Poepp. (<i>A. arvensis</i>), a Caribbean plant traditionally used for disease treatment. The dried plant powder was subjected t...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/23/7882 |
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| author | Valendy Thesnor Roland Molinié Ryland T. Giebelhaus A. Paulina de la Mata Espinosa James J. Harynuk David Bénimélis Bérénice Vanhoye Catherine Dunyach-Rémy Muriel Sylvestre Yvens Cheremond Patrick Meffre Gerardo Cebrián-Torrejón Zohra Benfodda |
| author_facet | Valendy Thesnor Roland Molinié Ryland T. Giebelhaus A. Paulina de la Mata Espinosa James J. Harynuk David Bénimélis Bérénice Vanhoye Catherine Dunyach-Rémy Muriel Sylvestre Yvens Cheremond Patrick Meffre Gerardo Cebrián-Torrejón Zohra Benfodda |
| author_sort | Valendy Thesnor |
| collection | DOAJ |
| description | The search for potent antimicrobial compounds is critical in the face of growing antibiotic resistance. This study explores <i>Acalypha arvensis</i> Poepp. (<i>A. arvensis</i>), a Caribbean plant traditionally used for disease treatment. The dried plant powder was subjected to successive extractions using different solvents: hexane (F1), dichloromethane (F2), methanol (F3), a 50:50 mixture of methanol and water (F4), and water (F5). Additionally, a parallel extraction was conducted using a 50:50 mixture of methanol and chloroform (F6). All the fractions were evaluated for their antimicrobial activity, and the F6 fraction was characterized using untargeted metabolomics using SPME-GC×GC-TOFMS. The extracts of <i>A. arvensis</i> F3, F4, and F5 showed antibacterial activity against <i>Staphylococcus aureus</i> ATCC 25923 (5 mg/mL), MRSA BA22038 (5 mg/mL), and <i>Pseudomonas aeruginosa</i> ATCC 27853 (10 mg/mL), and fraction F6 showed antibacterial activity against <i>Staphylococcus aureus</i> ATCC 29213 (2 mg/mL), <i>Escherichia coli</i> ATCC 25922 (20 mg/mL), <i>Pseudomonas aeruginosa</i> ATCC 27853 (10 mg/mL), <i>Enterococcus faecalis</i> ATCC 29212 (10 mg/mL), <i>Staphylococcus aureus</i> 024 (2 mg/mL), and <i>Staphylococcus aureus</i> 003 (2 mg/mL). Metabolomic analysis of F6 revealed 2861 peaks with 58 identified compounds through SPME and 3654 peaks with 29 identified compounds through derivatization. The compounds included methyl ester fatty acids, ethyl ester fatty acids, terpenes, ketones, sugars, amino acids, and fatty acids. This study represents the first exploration of <i>A. arvensis</i> metabolomics and its antimicrobial potential, providing valuable insights for plant classification, phytochemical research, and drug discovery. |
| first_indexed | 2024-03-09T01:45:56Z |
| format | Article |
| id | doaj.art-62e5fcd0952a42b9bed9774d0331b7ce |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T01:45:56Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-62e5fcd0952a42b9bed9774d0331b7ce2023-12-08T15:22:38ZengMDPI AGMolecules1420-30492023-11-012823788210.3390/molecules28237882Antibacterial Activity and Untargeted Metabolomics Profiling of <i>Acalypha arvensis</i> PoeppValendy Thesnor0Roland Molinié1Ryland T. Giebelhaus2A. Paulina de la Mata Espinosa3James J. Harynuk4David Bénimélis5Bérénice Vanhoye6Catherine Dunyach-Rémy7Muriel Sylvestre8Yvens Cheremond9Patrick Meffre10Gerardo Cebrián-Torrejón11Zohra Benfodda12UPR Chrome, University Nimes, CEDEX 1, 30021 Nîmes, FranceUMR INRAE 1158 Transfrontalière BioEcoAgro, BIOlogie des Plantes et Innovation (BIOPI), UPJV, UFR de Pharmacie, 80037 Amiens, FranceDepartment of Chemistry, University of Alberta, Edmonton, AB T6G 2N4, CanadaDepartment of Chemistry, University of Alberta, Edmonton, AB T6G 2N4, CanadaDepartment of Chemistry, University of Alberta, Edmonton, AB T6G 2N4, CanadaUPR Chrome, University Nimes, CEDEX 1, 30021 Nîmes, FranceUMR INRAE 1158 Transfrontalière BioEcoAgro, BIOlogie des Plantes et Innovation (BIOPI), UPJV, UFR de Pharmacie, 80037 Amiens, FranceCHU Nîmes—Hôpital Universitaire Carémeau, CEDEX, 30900 Nîmes, FranceCOVACHIM-M2E Laboratory EA 3592, Department of Chemistry, UFR SEN, Fouillole Campus, University of Antilles, CEDEX, 97110 Pointe-à-Pitre, FranceURE, Université d’État d’Haïti, Port-au-Prince HT6110, HaitiUPR Chrome, University Nimes, CEDEX 1, 30021 Nîmes, FranceCOVACHIM-M2E Laboratory EA 3592, Department of Chemistry, UFR SEN, Fouillole Campus, University of Antilles, CEDEX, 97110 Pointe-à-Pitre, FranceUPR Chrome, University Nimes, CEDEX 1, 30021 Nîmes, FranceThe search for potent antimicrobial compounds is critical in the face of growing antibiotic resistance. This study explores <i>Acalypha arvensis</i> Poepp. (<i>A. arvensis</i>), a Caribbean plant traditionally used for disease treatment. The dried plant powder was subjected to successive extractions using different solvents: hexane (F1), dichloromethane (F2), methanol (F3), a 50:50 mixture of methanol and water (F4), and water (F5). Additionally, a parallel extraction was conducted using a 50:50 mixture of methanol and chloroform (F6). All the fractions were evaluated for their antimicrobial activity, and the F6 fraction was characterized using untargeted metabolomics using SPME-GC×GC-TOFMS. The extracts of <i>A. arvensis</i> F3, F4, and F5 showed antibacterial activity against <i>Staphylococcus aureus</i> ATCC 25923 (5 mg/mL), MRSA BA22038 (5 mg/mL), and <i>Pseudomonas aeruginosa</i> ATCC 27853 (10 mg/mL), and fraction F6 showed antibacterial activity against <i>Staphylococcus aureus</i> ATCC 29213 (2 mg/mL), <i>Escherichia coli</i> ATCC 25922 (20 mg/mL), <i>Pseudomonas aeruginosa</i> ATCC 27853 (10 mg/mL), <i>Enterococcus faecalis</i> ATCC 29212 (10 mg/mL), <i>Staphylococcus aureus</i> 024 (2 mg/mL), and <i>Staphylococcus aureus</i> 003 (2 mg/mL). Metabolomic analysis of F6 revealed 2861 peaks with 58 identified compounds through SPME and 3654 peaks with 29 identified compounds through derivatization. The compounds included methyl ester fatty acids, ethyl ester fatty acids, terpenes, ketones, sugars, amino acids, and fatty acids. This study represents the first exploration of <i>A. arvensis</i> metabolomics and its antimicrobial potential, providing valuable insights for plant classification, phytochemical research, and drug discovery.https://www.mdpi.com/1420-3049/28/23/7882<i>Acalypha arvensis</i> Poepp.antibacterial activitySPME-GC×GC-TOFMSuntargeted metabolomicsGuadeloupe |
| spellingShingle | Valendy Thesnor Roland Molinié Ryland T. Giebelhaus A. Paulina de la Mata Espinosa James J. Harynuk David Bénimélis Bérénice Vanhoye Catherine Dunyach-Rémy Muriel Sylvestre Yvens Cheremond Patrick Meffre Gerardo Cebrián-Torrejón Zohra Benfodda Antibacterial Activity and Untargeted Metabolomics Profiling of <i>Acalypha arvensis</i> Poepp Molecules <i>Acalypha arvensis</i> Poepp. antibacterial activity SPME-GC×GC-TOFMS untargeted metabolomics Guadeloupe |
| title | Antibacterial Activity and Untargeted Metabolomics Profiling of <i>Acalypha arvensis</i> Poepp |
| title_full | Antibacterial Activity and Untargeted Metabolomics Profiling of <i>Acalypha arvensis</i> Poepp |
| title_fullStr | Antibacterial Activity and Untargeted Metabolomics Profiling of <i>Acalypha arvensis</i> Poepp |
| title_full_unstemmed | Antibacterial Activity and Untargeted Metabolomics Profiling of <i>Acalypha arvensis</i> Poepp |
| title_short | Antibacterial Activity and Untargeted Metabolomics Profiling of <i>Acalypha arvensis</i> Poepp |
| title_sort | antibacterial activity and untargeted metabolomics profiling of i acalypha arvensis i poepp |
| topic | <i>Acalypha arvensis</i> Poepp. antibacterial activity SPME-GC×GC-TOFMS untargeted metabolomics Guadeloupe |
| url | https://www.mdpi.com/1420-3049/28/23/7882 |
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