Biodegradation Potential and Putative Catabolic Genes of Culturable Bacteria from an Alpine Deciduous Forest Site
Microbiota from Alpine forest soils are key players in carbon cycling, which can be greatly affected by climate change. The aim of this study was to evaluate the degradation potential of culturable bacterial strains isolated from an alpine deciduous forest site. Fifty-five strains were studied with...
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MDPI AG
2021-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/9/1920 |
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| author | Caroline Poyntner Andrea Kutzner Rosa Margesin |
| author_facet | Caroline Poyntner Andrea Kutzner Rosa Margesin |
| author_sort | Caroline Poyntner |
| collection | DOAJ |
| description | Microbiota from Alpine forest soils are key players in carbon cycling, which can be greatly affected by climate change. The aim of this study was to evaluate the degradation potential of culturable bacterial strains isolated from an alpine deciduous forest site. Fifty-five strains were studied with regard to their phylogenetic position, growth temperature range and degradation potential for organic compounds (microtiter scale screening for lignin sulfonic acid, catechol, phenol, bisphenol A) at low (5 °C) and moderate (20 °C) temperature. Additionally, the presence of putative catabolic genes (catechol-1,2-dioxygenase, multicomponent phenol hydroxylase, protocatechuate-3,4-dioxygenase) involved in the degradation of these organic compounds was determined through PCR. The results show the importance of the <i>Proteobacteria</i> phylum as its representatives did show good capabilities for biodegradation and good growth at −5 °C. Overall, 82% of strains were able to use at least one of the tested organic compounds as their sole carbon source. The presence of putative catabolic genes could be shown over a broad range of strains and in relation to their degradation abilities. Subsequently performed gene sequencing indicated horizontal gene transfer for catechol-1,2-dioxygenase and protocatechuate-3,4-dioxygenase. The results show the great benefit of combining molecular and culture-based techniques. |
| first_indexed | 2024-03-10T07:25:05Z |
| format | Article |
| id | doaj.art-62e66cd7ce9a42ab8aefe01814b7dbad |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T07:25:05Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-62e66cd7ce9a42ab8aefe01814b7dbad2023-11-22T14:19:07ZengMDPI AGMicroorganisms2076-26072021-09-0199192010.3390/microorganisms9091920Biodegradation Potential and Putative Catabolic Genes of Culturable Bacteria from an Alpine Deciduous Forest SiteCaroline Poyntner0Andrea Kutzner1Rosa Margesin2Department of Microbiology, University of Innsbruck, Technikerstrasse 25, 6020 Innsbruck, AustriaDepartment of Microbiology, University of Innsbruck, Technikerstrasse 25, 6020 Innsbruck, AustriaDepartment of Microbiology, University of Innsbruck, Technikerstrasse 25, 6020 Innsbruck, AustriaMicrobiota from Alpine forest soils are key players in carbon cycling, which can be greatly affected by climate change. The aim of this study was to evaluate the degradation potential of culturable bacterial strains isolated from an alpine deciduous forest site. Fifty-five strains were studied with regard to their phylogenetic position, growth temperature range and degradation potential for organic compounds (microtiter scale screening for lignin sulfonic acid, catechol, phenol, bisphenol A) at low (5 °C) and moderate (20 °C) temperature. Additionally, the presence of putative catabolic genes (catechol-1,2-dioxygenase, multicomponent phenol hydroxylase, protocatechuate-3,4-dioxygenase) involved in the degradation of these organic compounds was determined through PCR. The results show the importance of the <i>Proteobacteria</i> phylum as its representatives did show good capabilities for biodegradation and good growth at −5 °C. Overall, 82% of strains were able to use at least one of the tested organic compounds as their sole carbon source. The presence of putative catabolic genes could be shown over a broad range of strains and in relation to their degradation abilities. Subsequently performed gene sequencing indicated horizontal gene transfer for catechol-1,2-dioxygenase and protocatechuate-3,4-dioxygenase. The results show the great benefit of combining molecular and culture-based techniques.https://www.mdpi.com/2076-2607/9/9/1920<i>Pseudomonas</i><i>Rhodococcus</i><i>Collimonas</i>biodegradationphenolcatechol |
| spellingShingle | Caroline Poyntner Andrea Kutzner Rosa Margesin Biodegradation Potential and Putative Catabolic Genes of Culturable Bacteria from an Alpine Deciduous Forest Site Microorganisms <i>Pseudomonas</i> <i>Rhodococcus</i> <i>Collimonas</i> biodegradation phenol catechol |
| title | Biodegradation Potential and Putative Catabolic Genes of Culturable Bacteria from an Alpine Deciduous Forest Site |
| title_full | Biodegradation Potential and Putative Catabolic Genes of Culturable Bacteria from an Alpine Deciduous Forest Site |
| title_fullStr | Biodegradation Potential and Putative Catabolic Genes of Culturable Bacteria from an Alpine Deciduous Forest Site |
| title_full_unstemmed | Biodegradation Potential and Putative Catabolic Genes of Culturable Bacteria from an Alpine Deciduous Forest Site |
| title_short | Biodegradation Potential and Putative Catabolic Genes of Culturable Bacteria from an Alpine Deciduous Forest Site |
| title_sort | biodegradation potential and putative catabolic genes of culturable bacteria from an alpine deciduous forest site |
| topic | <i>Pseudomonas</i> <i>Rhodococcus</i> <i>Collimonas</i> biodegradation phenol catechol |
| url | https://www.mdpi.com/2076-2607/9/9/1920 |
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