A Patient With Multiple Carbapenemase Producers Including an Unusual Citrobacter sedlakii Hosting an IncC blaNDM-1- and armA-carrying Plasmid
Background. Patients colonized with multiple species of carbapenemase-producing Enterobacterales (CPE) are increasingly observed. This phenomenon can be due to the high local prevalence of these pathogens, the presence of important host risk factors, and the great genetic promiscuity of some carbap...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Case Western Reserve University
2021-11-01
|
| Series: | Pathogens and Immunity |
| Subjects: | |
| Online Access: | https://www.paijournal.com/index.php/paijournal/article/view/482 |
| _version_ | 1828433115354234880 |
|---|---|
| author | Aline Moser Peter Keller Edgar I. Campos-Madueno Laurent Poirel Patrice Nordmann Andrea Endimiani |
| author_facet | Aline Moser Peter Keller Edgar I. Campos-Madueno Laurent Poirel Patrice Nordmann Andrea Endimiani |
| author_sort | Aline Moser |
| collection | DOAJ |
| description |
Background. Patients colonized with multiple species of carbapenemase-producing Enterobacterales (CPE) are increasingly observed. This phenomenon can be due to the high local prevalence of these pathogens, the presence of important host risk factors, and the great genetic promiscuity of some carbapenemase genes.
Methods. We analyzed 4 CPE (Escherichia coli, Klebsiella pneumoniae, Providencia stuartii, Citrobacter sedlakii), 1 extended-spectrum cephalosporin-resistant K. pneumoniae (ESC-R-Kp), and 1 carbapenemase-producing Acinetobacter baumannii simultaneously isolated from a patient transferred from Macedonia. Susceptibility tests were performed using a microdilution MIC system. The complete genome sequences were obtained by using both short-read and long-read whole-genome sequencing technologies.
Results. All CPE presented high-level resistance to all aminoglycosides due to the expression of the armA 16S rRNA methylase. In C. sedlakii and E. coli (ST69), both the carbapenemase blaNDM-1 and armA genes were located on an identical IncC plasmid of type 1a. The K. pneumoniae (ST268) and P. stuartii carried chromosomal blaNDM-1 and blaOXA-48, respectively, while the ESC-R-Kp (ST395) harbored a plasmid-located blaCTX-M-15. In the latter 3 isolates, armA-harboring IncC plasmids similar to plasmids found in C. sedlakii and E. coli were also detected. The A. baumannii strain possessed the blaOXA-40 carbapenemase gene.
Conclusions. The characterization of the genetic organization of IncC-type plasmids harbored by 3 different species from the same patient offered insights into the evolution of these broad- host-range plasmids. Moreover, we characterized here the first complete genome sequence of a carbapenemase-producing C. sedlakii strain, providing a reference for future studies on this rarely reported species.
|
| first_indexed | 2024-12-10T18:30:35Z |
| format | Article |
| id | doaj.art-62e788e7f808487aa475f8a3c3a6e349 |
| institution | Directory Open Access Journal |
| issn | 2469-2964 |
| language | English |
| last_indexed | 2024-12-10T18:30:35Z |
| publishDate | 2021-11-01 |
| publisher | Case Western Reserve University |
| record_format | Article |
| series | Pathogens and Immunity |
| spelling | doaj.art-62e788e7f808487aa475f8a3c3a6e3492022-12-22T01:37:57ZengCase Western Reserve UniversityPathogens and Immunity2469-29642021-11-016210.20411/pai.v6i2.482A Patient With Multiple Carbapenemase Producers Including an Unusual Citrobacter sedlakii Hosting an IncC blaNDM-1- and armA-carrying PlasmidAline Moser0Peter Keller1Edgar I. Campos-Madueno2Laurent Poirel3Patrice Nordmann4Andrea Endimiani5Institute for Infectious Diseases (IFIK), University of Bern, Bern, SwitzerlandInstitute for Infectious Diseases (IFIK), University of Bern, Bern, SwitzerlandInstitute for Infectious Diseases (IFIK), University of Bern, Bern, Switzerland; Graduate School of Cellular and Biomedical Sciences, University of Bern, Bern, SwitzerlandEmerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Fribourg, Switzerland; French INSERM European Unit, University of Fribourg (LEA-IAME), Fribourg, Switzerland; National Reference Center for Emerging Antibiotic Resistance (NARA), Fribourg, SwitzerlandEmerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Fribourg, Switzerland; French INSERM European Unit, University of Fribourg (LEA-IAME), Fribourg, Switzerland; National Reference Center for Emerging Antibiotic Resistance (NARA), Fribourg, SwitzerlandInstitute for Infectious Diseases (IFIK), University of Bern, Bern, Switzerland Background. Patients colonized with multiple species of carbapenemase-producing Enterobacterales (CPE) are increasingly observed. This phenomenon can be due to the high local prevalence of these pathogens, the presence of important host risk factors, and the great genetic promiscuity of some carbapenemase genes. Methods. We analyzed 4 CPE (Escherichia coli, Klebsiella pneumoniae, Providencia stuartii, Citrobacter sedlakii), 1 extended-spectrum cephalosporin-resistant K. pneumoniae (ESC-R-Kp), and 1 carbapenemase-producing Acinetobacter baumannii simultaneously isolated from a patient transferred from Macedonia. Susceptibility tests were performed using a microdilution MIC system. The complete genome sequences were obtained by using both short-read and long-read whole-genome sequencing technologies. Results. All CPE presented high-level resistance to all aminoglycosides due to the expression of the armA 16S rRNA methylase. In C. sedlakii and E. coli (ST69), both the carbapenemase blaNDM-1 and armA genes were located on an identical IncC plasmid of type 1a. The K. pneumoniae (ST268) and P. stuartii carried chromosomal blaNDM-1 and blaOXA-48, respectively, while the ESC-R-Kp (ST395) harbored a plasmid-located blaCTX-M-15. In the latter 3 isolates, armA-harboring IncC plasmids similar to plasmids found in C. sedlakii and E. coli were also detected. The A. baumannii strain possessed the blaOXA-40 carbapenemase gene. Conclusions. The characterization of the genetic organization of IncC-type plasmids harbored by 3 different species from the same patient offered insights into the evolution of these broad- host-range plasmids. Moreover, we characterized here the first complete genome sequence of a carbapenemase-producing C. sedlakii strain, providing a reference for future studies on this rarely reported species. https://www.paijournal.com/index.php/paijournal/article/view/482carbapenemases;NDM-1;Enterobacterales;ArmA;plasmid;CPE |
| spellingShingle | Aline Moser Peter Keller Edgar I. Campos-Madueno Laurent Poirel Patrice Nordmann Andrea Endimiani A Patient With Multiple Carbapenemase Producers Including an Unusual Citrobacter sedlakii Hosting an IncC blaNDM-1- and armA-carrying Plasmid Pathogens and Immunity carbapenemases; NDM-1; Enterobacterales; ArmA; plasmid; CPE |
| title | A Patient With Multiple Carbapenemase Producers Including an Unusual Citrobacter sedlakii Hosting an IncC blaNDM-1- and armA-carrying Plasmid |
| title_full | A Patient With Multiple Carbapenemase Producers Including an Unusual Citrobacter sedlakii Hosting an IncC blaNDM-1- and armA-carrying Plasmid |
| title_fullStr | A Patient With Multiple Carbapenemase Producers Including an Unusual Citrobacter sedlakii Hosting an IncC blaNDM-1- and armA-carrying Plasmid |
| title_full_unstemmed | A Patient With Multiple Carbapenemase Producers Including an Unusual Citrobacter sedlakii Hosting an IncC blaNDM-1- and armA-carrying Plasmid |
| title_short | A Patient With Multiple Carbapenemase Producers Including an Unusual Citrobacter sedlakii Hosting an IncC blaNDM-1- and armA-carrying Plasmid |
| title_sort | patient with multiple carbapenemase producers including an unusual citrobacter sedlakii hosting an incc blandm 1 and arma carrying plasmid |
| topic | carbapenemases; NDM-1; Enterobacterales; ArmA; plasmid; CPE |
| url | https://www.paijournal.com/index.php/paijournal/article/view/482 |
| work_keys_str_mv | AT alinemoser apatientwithmultiplecarbapenemaseproducersincludinganunusualcitrobactersedlakiihostinganinccblandm1andarmacarryingplasmid AT peterkeller apatientwithmultiplecarbapenemaseproducersincludinganunusualcitrobactersedlakiihostinganinccblandm1andarmacarryingplasmid AT edgaricamposmadueno apatientwithmultiplecarbapenemaseproducersincludinganunusualcitrobactersedlakiihostinganinccblandm1andarmacarryingplasmid AT laurentpoirel apatientwithmultiplecarbapenemaseproducersincludinganunusualcitrobactersedlakiihostinganinccblandm1andarmacarryingplasmid AT patricenordmann apatientwithmultiplecarbapenemaseproducersincludinganunusualcitrobactersedlakiihostinganinccblandm1andarmacarryingplasmid AT andreaendimiani apatientwithmultiplecarbapenemaseproducersincludinganunusualcitrobactersedlakiihostinganinccblandm1andarmacarryingplasmid AT alinemoser patientwithmultiplecarbapenemaseproducersincludinganunusualcitrobactersedlakiihostinganinccblandm1andarmacarryingplasmid AT peterkeller patientwithmultiplecarbapenemaseproducersincludinganunusualcitrobactersedlakiihostinganinccblandm1andarmacarryingplasmid AT edgaricamposmadueno patientwithmultiplecarbapenemaseproducersincludinganunusualcitrobactersedlakiihostinganinccblandm1andarmacarryingplasmid AT laurentpoirel patientwithmultiplecarbapenemaseproducersincludinganunusualcitrobactersedlakiihostinganinccblandm1andarmacarryingplasmid AT patricenordmann patientwithmultiplecarbapenemaseproducersincludinganunusualcitrobactersedlakiihostinganinccblandm1andarmacarryingplasmid AT andreaendimiani patientwithmultiplecarbapenemaseproducersincludinganunusualcitrobactersedlakiihostinganinccblandm1andarmacarryingplasmid |