Slit/Robo Signaling Regulates Cell Fate Decisions in the Intestinal Stem Cell Lineage of Drosophila
In order to maintain tissue homeostasis, cell fate decisions within stem cell lineages have to respond to the needs of the tissue. This coordination of lineage choices with regenerative demand remains poorly characterized. Here, we identify a signal from enteroendocrine cells (EEs) that controls lin...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2014-06-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124714003994 |
| _version_ | 1818258354633441280 |
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| author | Benoît Biteau Heinrich Jasper |
| author_facet | Benoît Biteau Heinrich Jasper |
| author_sort | Benoît Biteau |
| collection | DOAJ |
| description | In order to maintain tissue homeostasis, cell fate decisions within stem cell lineages have to respond to the needs of the tissue. This coordination of lineage choices with regenerative demand remains poorly characterized. Here, we identify a signal from enteroendocrine cells (EEs) that controls lineage specification in the Drosophila intestine. We find that EEs secrete Slit, a ligand for the Robo2 receptor in intestinal stem cells (ISCs) that limits ISC commitment to the endocrine lineage, establishing negative feedback control of EE regeneration. Furthermore, we show that this lineage decision is made within ISCs and requires induction of the transcription factor Prospero in ISCs. Our work identifies a function for the conserved Slit/Robo pathway in the regulation of adult stem cells, establishing negative feedback control of ISC lineage specification as a critical strategy to preserve tissue homeostasis. Our results further amend the current understanding of cell fate commitment within the Drosophila ISC lineage. |
| first_indexed | 2024-12-12T17:58:13Z |
| format | Article |
| id | doaj.art-62f16b074a0441c69053143427db099c |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-12T17:58:13Z |
| publishDate | 2014-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-62f16b074a0441c69053143427db099c2022-12-22T00:16:40ZengElsevierCell Reports2211-12472014-06-01761867187510.1016/j.celrep.2014.05.024Slit/Robo Signaling Regulates Cell Fate Decisions in the Intestinal Stem Cell Lineage of DrosophilaBenoît Biteau0Heinrich Jasper1Department of Biomedical Genetics, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USABuck Institute for Research on Aging, 8001 Redwood Boulevard, Novato, CA 94945, USAIn order to maintain tissue homeostasis, cell fate decisions within stem cell lineages have to respond to the needs of the tissue. This coordination of lineage choices with regenerative demand remains poorly characterized. Here, we identify a signal from enteroendocrine cells (EEs) that controls lineage specification in the Drosophila intestine. We find that EEs secrete Slit, a ligand for the Robo2 receptor in intestinal stem cells (ISCs) that limits ISC commitment to the endocrine lineage, establishing negative feedback control of EE regeneration. Furthermore, we show that this lineage decision is made within ISCs and requires induction of the transcription factor Prospero in ISCs. Our work identifies a function for the conserved Slit/Robo pathway in the regulation of adult stem cells, establishing negative feedback control of ISC lineage specification as a critical strategy to preserve tissue homeostasis. Our results further amend the current understanding of cell fate commitment within the Drosophila ISC lineage.http://www.sciencedirect.com/science/article/pii/S2211124714003994 |
| spellingShingle | Benoît Biteau Heinrich Jasper Slit/Robo Signaling Regulates Cell Fate Decisions in the Intestinal Stem Cell Lineage of Drosophila Cell Reports |
| title | Slit/Robo Signaling Regulates Cell Fate Decisions in the Intestinal Stem Cell Lineage of Drosophila |
| title_full | Slit/Robo Signaling Regulates Cell Fate Decisions in the Intestinal Stem Cell Lineage of Drosophila |
| title_fullStr | Slit/Robo Signaling Regulates Cell Fate Decisions in the Intestinal Stem Cell Lineage of Drosophila |
| title_full_unstemmed | Slit/Robo Signaling Regulates Cell Fate Decisions in the Intestinal Stem Cell Lineage of Drosophila |
| title_short | Slit/Robo Signaling Regulates Cell Fate Decisions in the Intestinal Stem Cell Lineage of Drosophila |
| title_sort | slit robo signaling regulates cell fate decisions in the intestinal stem cell lineage of drosophila |
| url | http://www.sciencedirect.com/science/article/pii/S2211124714003994 |
| work_keys_str_mv | AT benoitbiteau slitrobosignalingregulatescellfatedecisionsintheintestinalstemcelllineageofdrosophila AT heinrichjasper slitrobosignalingregulatescellfatedecisionsintheintestinalstemcelllineageofdrosophila |