Bessel Beam Illumination Reduces Random and Systematic Errors in Quantitative Functional Studies Using Light-Sheet Microscopy

Bessel Beam Illumination Reduces Random and Systematic Errors in Quantitative Functional Studies Using Light-Sheet Microscopy

Light-sheet microscopy (LSM), in combination with intrinsically transparent zebrafish larvae, is a method of choice to observe brain function with high frame rates at cellular resolution. Inherently to LSM, however, residual opaque objects cause stripe artifacts, which obscure features of interest a...

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Bibliographic Details
Main Authors: M. Caroline Müllenbroich, Lapo Turrini, Ludovico Silvestri, Tommaso Alterini, Ali Gheisari, Natascia Tiso, Francesco Vanzi, Leonardo Sacconi, Francesco S. Pavone
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-09-01
Series:Frontiers in Cellular Neuroscience
Subjects:
spontaneous activity
zebrafish
principle component analysis
light-sheet microscopy
functional imaging
Bessel beams
Online Access:https://www.frontiersin.org/article/10.3389/fncel.2018.00315/full

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