Simultaneous Detection of Antigen-Specific IgG- and IgM-Secreting Cells with a B Cell Fluorospot Assay
The traditional enzyme-linked immunospot (ELISpot) assay is the gold standard for the enumeration of antigen-specific B cells. Since B cell availability from biological samples is often limited, either because of sample size/volume or the need of performing multiple analyses on the same sample, the...
Auteurs principaux: | , |
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Format: | Article |
Langue: | English |
Publié: |
MDPI AG
2012-03-01
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Collection: | Cells |
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Accès en ligne: | http://www.mdpi.com/2073-4409/1/1/15 |