Study of interaction between nicotinamide and human serum albumin using spectroscopic techniques and molecular docking simulation simulation
Human serum albumin is one of the most important blood proteins that has the ability to bind a wide range of compounds and different drugs. Hence, knowing how drugs bind to albumin is crucial to understand their pharmacokinetics and pharmacodynamic properties. The binding of drugs to protein affects...
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| Format: | Article |
| Language: | English |
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Kharazmi University
2022-12-01
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| Series: | یافتههای نوین در علوم زیستی |
| Subjects: | |
| Online Access: | http://nbr.khu.ac.ir/article-1-3502-en.html |
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| author | Golnaz Parvizi Fard Lale Solouki Mostafa Zakariazadeh Hossein Haghaei Somaieh Soltani |
| author_facet | Golnaz Parvizi Fard Lale Solouki Mostafa Zakariazadeh Hossein Haghaei Somaieh Soltani |
| author_sort | Golnaz Parvizi Fard |
| collection | DOAJ |
| description | Human serum albumin is one of the most important blood proteins that has the ability to bind a wide range of compounds and different drugs. Hence, knowing how drugs bind to albumin is crucial to understand their pharmacokinetics and pharmacodynamic properties. The binding of drugs to protein affects the drug's excretion, distribution and interaction in the target tissues. Nicotinamide (NA) is a safe and inexpensive medical supplement that used to prevent and treat vitamin B3 deficiency. In this research, the molecular mechanism of the interaction between nicotinamide and human serum albumin was studied by the utilization of spectroscopic and molecular docking methods. The effects of temperature, acidic/basic pHs, metal ions, urea, and glucose on the interaction between nicotinamide and human serum albumin were also investigated. The spectroscopic studies indicated that the interaction between nicotinamide and human serum albumin is mainly controled by hydrophobic forces and the interaction is spontaneous. The number of binding site and binding constant is 1 and 4.6×104 (L/mol), respectively, which were increased in the presence of glucose. The presence of metallic ions and basic pH decreased the binding constant of nicotinamide to albumin. The obtained results indicated that nicotinamide tend to binds to the similar sites wherever the molecules with acidic moieties bind. The results could be helpful to interpret the mechanisms of actions of nicotinamide in the various physiological phenomena in the human body. |
| first_indexed | 2024-04-10T21:53:21Z |
| format | Article |
| id | doaj.art-62f3b416814641389af8776fa3511bfd |
| institution | Directory Open Access Journal |
| issn | 2423-6330 2476-7115 |
| language | English |
| last_indexed | 2024-04-10T21:53:21Z |
| publishDate | 2022-12-01 |
| publisher | Kharazmi University |
| record_format | Article |
| series | یافتههای نوین در علوم زیستی |
| spelling | doaj.art-62f3b416814641389af8776fa3511bfd2023-01-18T11:26:53ZengKharazmi Universityیافتههای نوین در علوم زیستی2423-63302476-71152022-12-0193153168Study of interaction between nicotinamide and human serum albumin using spectroscopic techniques and molecular docking simulation simulationGolnaz Parvizi Fard0Lale Solouki1Mostafa Zakariazadeh2Hossein Haghaei3Somaieh Soltani4 Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Department of Biology, Faculty of Sciences, Payame Noor University, Tehran, Iran Department of Nutrition, Faculty of Nutrition and Food Sciences, Tabriz University of Medical Sciences, Tabriz, Iran Department of Medicinal Chemistry, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran Human serum albumin is one of the most important blood proteins that has the ability to bind a wide range of compounds and different drugs. Hence, knowing how drugs bind to albumin is crucial to understand their pharmacokinetics and pharmacodynamic properties. The binding of drugs to protein affects the drug's excretion, distribution and interaction in the target tissues. Nicotinamide (NA) is a safe and inexpensive medical supplement that used to prevent and treat vitamin B3 deficiency. In this research, the molecular mechanism of the interaction between nicotinamide and human serum albumin was studied by the utilization of spectroscopic and molecular docking methods. The effects of temperature, acidic/basic pHs, metal ions, urea, and glucose on the interaction between nicotinamide and human serum albumin were also investigated. The spectroscopic studies indicated that the interaction between nicotinamide and human serum albumin is mainly controled by hydrophobic forces and the interaction is spontaneous. The number of binding site and binding constant is 1 and 4.6×104 (L/mol), respectively, which were increased in the presence of glucose. The presence of metallic ions and basic pH decreased the binding constant of nicotinamide to albumin. The obtained results indicated that nicotinamide tend to binds to the similar sites wherever the molecules with acidic moieties bind. The results could be helpful to interpret the mechanisms of actions of nicotinamide in the various physiological phenomena in the human body.http://nbr.khu.ac.ir/article-1-3502-en.htmlcarrier proteindrug-protein interactionmolecular simulationspectroscopyvitamin b3 |
| spellingShingle | Golnaz Parvizi Fard Lale Solouki Mostafa Zakariazadeh Hossein Haghaei Somaieh Soltani Study of interaction between nicotinamide and human serum albumin using spectroscopic techniques and molecular docking simulation simulation یافتههای نوین در علوم زیستی carrier protein drug-protein interaction molecular simulation spectroscopy vitamin b3 |
| title | Study of interaction between nicotinamide and human serum albumin using spectroscopic techniques and molecular docking simulation simulation |
| title_full | Study of interaction between nicotinamide and human serum albumin using spectroscopic techniques and molecular docking simulation simulation |
| title_fullStr | Study of interaction between nicotinamide and human serum albumin using spectroscopic techniques and molecular docking simulation simulation |
| title_full_unstemmed | Study of interaction between nicotinamide and human serum albumin using spectroscopic techniques and molecular docking simulation simulation |
| title_short | Study of interaction between nicotinamide and human serum albumin using spectroscopic techniques and molecular docking simulation simulation |
| title_sort | study of interaction between nicotinamide and human serum albumin using spectroscopic techniques and molecular docking simulation simulation |
| topic | carrier protein drug-protein interaction molecular simulation spectroscopy vitamin b3 |
| url | http://nbr.khu.ac.ir/article-1-3502-en.html |
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