Neurosecretory Protein GM–Expressing Neurons Participate in Lipid Storage and Inflammation in Newly Developed Cre Driver Male Mice
Obesity induces inflammation in the hypothalamus and adipose tissue, resulting in metabolic disorders. A novel hypothalamic neuropeptide, neurosecretory protein GM (NPGM), was previously identified in the hypothalamus of vertebrates. While NPGM plays an important role in lipid metabolism in chicks,...
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/12/3230 |
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| author | Yuki Narimatsu Masaki Kato Eiko Iwakoshi-Ukena Shogo Moriwaki Ayano Ogasawara Megumi Furumitsu Kazuyoshi Ukena |
| author_facet | Yuki Narimatsu Masaki Kato Eiko Iwakoshi-Ukena Shogo Moriwaki Ayano Ogasawara Megumi Furumitsu Kazuyoshi Ukena |
| author_sort | Yuki Narimatsu |
| collection | DOAJ |
| description | Obesity induces inflammation in the hypothalamus and adipose tissue, resulting in metabolic disorders. A novel hypothalamic neuropeptide, neurosecretory protein GM (NPGM), was previously identified in the hypothalamus of vertebrates. While NPGM plays an important role in lipid metabolism in chicks, its metabolic regulatory effects in mammals remain unclear. In this study, a novel Cre driver line, NPGM-Cre, was generated for cell-specific manipulation. Cre-dependent overexpression of <i>Npgm</i> led to fat accumulation without increased food consumption in male NPGM-Cre mice. Chemogenetic activation of NPGM neurons in the hypothalamus acutely promoted feeding behavior and chronically resulted in a transient increase in body mass gain. Furthermore, the ablated NPGM neurons exhibited a tendency to be glucose intolerant, with infiltration of proinflammatory macrophages into the adipose tissue. These results suggest that NPGM neurons may regulate lipid storage and inflammatory responses, thereby maintaining glucose homeostasis. |
| first_indexed | 2024-03-08T20:58:38Z |
| format | Article |
| id | doaj.art-62f81ee18082434c926148a91477fffe |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-08T20:58:38Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-62f81ee18082434c926148a91477fffe2023-12-22T13:55:02ZengMDPI AGBiomedicines2227-90592023-12-011112323010.3390/biomedicines11123230Neurosecretory Protein GM–Expressing Neurons Participate in Lipid Storage and Inflammation in Newly Developed Cre Driver Male MiceYuki Narimatsu0Masaki Kato1Eiko Iwakoshi-Ukena2Shogo Moriwaki3Ayano Ogasawara4Megumi Furumitsu5Kazuyoshi Ukena6Laboratory of Neurometabolism, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8521, JapanLaboratory of Neurometabolism, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8521, JapanLaboratory of Neurometabolism, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8521, JapanLaboratory of Neurometabolism, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8521, JapanLaboratory of Neurometabolism, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8521, JapanLaboratory of Neurometabolism, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8521, JapanLaboratory of Neurometabolism, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8521, JapanObesity induces inflammation in the hypothalamus and adipose tissue, resulting in metabolic disorders. A novel hypothalamic neuropeptide, neurosecretory protein GM (NPGM), was previously identified in the hypothalamus of vertebrates. While NPGM plays an important role in lipid metabolism in chicks, its metabolic regulatory effects in mammals remain unclear. In this study, a novel Cre driver line, NPGM-Cre, was generated for cell-specific manipulation. Cre-dependent overexpression of <i>Npgm</i> led to fat accumulation without increased food consumption in male NPGM-Cre mice. Chemogenetic activation of NPGM neurons in the hypothalamus acutely promoted feeding behavior and chronically resulted in a transient increase in body mass gain. Furthermore, the ablated NPGM neurons exhibited a tendency to be glucose intolerant, with infiltration of proinflammatory macrophages into the adipose tissue. These results suggest that NPGM neurons may regulate lipid storage and inflammatory responses, thereby maintaining glucose homeostasis.https://www.mdpi.com/2227-9059/11/12/3230neurosecretory protein GMhypothalamusneuropeptideobesitychronic inflammationchemogenetics |
| spellingShingle | Yuki Narimatsu Masaki Kato Eiko Iwakoshi-Ukena Shogo Moriwaki Ayano Ogasawara Megumi Furumitsu Kazuyoshi Ukena Neurosecretory Protein GM–Expressing Neurons Participate in Lipid Storage and Inflammation in Newly Developed Cre Driver Male Mice Biomedicines neurosecretory protein GM hypothalamus neuropeptide obesity chronic inflammation chemogenetics |
| title | Neurosecretory Protein GM–Expressing Neurons Participate in Lipid Storage and Inflammation in Newly Developed Cre Driver Male Mice |
| title_full | Neurosecretory Protein GM–Expressing Neurons Participate in Lipid Storage and Inflammation in Newly Developed Cre Driver Male Mice |
| title_fullStr | Neurosecretory Protein GM–Expressing Neurons Participate in Lipid Storage and Inflammation in Newly Developed Cre Driver Male Mice |
| title_full_unstemmed | Neurosecretory Protein GM–Expressing Neurons Participate in Lipid Storage and Inflammation in Newly Developed Cre Driver Male Mice |
| title_short | Neurosecretory Protein GM–Expressing Neurons Participate in Lipid Storage and Inflammation in Newly Developed Cre Driver Male Mice |
| title_sort | neurosecretory protein gm expressing neurons participate in lipid storage and inflammation in newly developed cre driver male mice |
| topic | neurosecretory protein GM hypothalamus neuropeptide obesity chronic inflammation chemogenetics |
| url | https://www.mdpi.com/2227-9059/11/12/3230 |
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