Testing biological actions of medicinal plants from northern Vietnam on zebrafish embryos and larvae: Developmental, behavioral, and putative therapeutical effects.
Evaluating the risks and benefits of using traditional medicinal plants is of utmost importance for a huge fraction of the human population, in particular in Northern Vietnam. Zebrafish are increasingly used as a simple vertebrate model for testing toxic and physiological effects of compounds, espec...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0294048&type=printable |
| _version_ | 1797394478867152896 |
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| author | My Hanh Tran Thi Van Anh Nguyen Hoang Giang Do Trung Kien Kieu Thi Kim Thanh Nguyen Hong Diep Le Gustavo Guerrero-Limon Laura Massoz Renaud Nivelle Jérémie Zappia Hai The Pham Lai Thanh Nguyen Marc Muller |
| author_facet | My Hanh Tran Thi Van Anh Nguyen Hoang Giang Do Trung Kien Kieu Thi Kim Thanh Nguyen Hong Diep Le Gustavo Guerrero-Limon Laura Massoz Renaud Nivelle Jérémie Zappia Hai The Pham Lai Thanh Nguyen Marc Muller |
| author_sort | My Hanh Tran |
| collection | DOAJ |
| description | Evaluating the risks and benefits of using traditional medicinal plants is of utmost importance for a huge fraction of the human population, in particular in Northern Vietnam. Zebrafish are increasingly used as a simple vertebrate model for testing toxic and physiological effects of compounds, especially on development. Here, we tested 12 ethanolic extracts from popular medicinal plants collected in northern Vietnam for their effects on zebrafish survival and development during the first 4 days after fertilization. We characterized more in detail their effects on epiboly, hatching, growth, necrosis, body curvature, angiogenesis, skeletal development and mostly increased movement behavior. Finally, we confirm the effect on epiboly caused by the Mahonia bealei extract by staining the actin filaments and performing whole genome gene expression analysis. Further, we show that this extract also inhibits cell migration of mouse embryo fibroblasts. Finally, we analyzed the chemical composition of the Mahonia bealei extract and test the effects of its major components. In conclusion, we show that traditional medicinal plant extracts are able to affect zebrafish early life stage development to various degrees. In addition, we show that an extract causing delay in epiboly also inhibits mammalian cell migration, suggesting that this effect may serve as a preliminary test for identifying extracts that inhibit cancer metastasis. |
| first_indexed | 2024-03-09T00:20:22Z |
| format | Article |
| id | doaj.art-62f9094ce1e84ce4ba0051661e7211b9 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-09T00:20:22Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-62f9094ce1e84ce4ba0051661e7211b92023-12-12T05:34:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-011811e029404810.1371/journal.pone.0294048Testing biological actions of medicinal plants from northern Vietnam on zebrafish embryos and larvae: Developmental, behavioral, and putative therapeutical effects.My Hanh TranThi Van Anh NguyenHoang Giang DoTrung Kien KieuThi Kim Thanh NguyenHong Diep LeGustavo Guerrero-LimonLaura MassozRenaud NivelleJérémie ZappiaHai The PhamLai Thanh NguyenMarc MullerEvaluating the risks and benefits of using traditional medicinal plants is of utmost importance for a huge fraction of the human population, in particular in Northern Vietnam. Zebrafish are increasingly used as a simple vertebrate model for testing toxic and physiological effects of compounds, especially on development. Here, we tested 12 ethanolic extracts from popular medicinal plants collected in northern Vietnam for their effects on zebrafish survival and development during the first 4 days after fertilization. We characterized more in detail their effects on epiboly, hatching, growth, necrosis, body curvature, angiogenesis, skeletal development and mostly increased movement behavior. Finally, we confirm the effect on epiboly caused by the Mahonia bealei extract by staining the actin filaments and performing whole genome gene expression analysis. Further, we show that this extract also inhibits cell migration of mouse embryo fibroblasts. Finally, we analyzed the chemical composition of the Mahonia bealei extract and test the effects of its major components. In conclusion, we show that traditional medicinal plant extracts are able to affect zebrafish early life stage development to various degrees. In addition, we show that an extract causing delay in epiboly also inhibits mammalian cell migration, suggesting that this effect may serve as a preliminary test for identifying extracts that inhibit cancer metastasis.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0294048&type=printable |
| spellingShingle | My Hanh Tran Thi Van Anh Nguyen Hoang Giang Do Trung Kien Kieu Thi Kim Thanh Nguyen Hong Diep Le Gustavo Guerrero-Limon Laura Massoz Renaud Nivelle Jérémie Zappia Hai The Pham Lai Thanh Nguyen Marc Muller Testing biological actions of medicinal plants from northern Vietnam on zebrafish embryos and larvae: Developmental, behavioral, and putative therapeutical effects. PLoS ONE |
| title | Testing biological actions of medicinal plants from northern Vietnam on zebrafish embryos and larvae: Developmental, behavioral, and putative therapeutical effects. |
| title_full | Testing biological actions of medicinal plants from northern Vietnam on zebrafish embryos and larvae: Developmental, behavioral, and putative therapeutical effects. |
| title_fullStr | Testing biological actions of medicinal plants from northern Vietnam on zebrafish embryos and larvae: Developmental, behavioral, and putative therapeutical effects. |
| title_full_unstemmed | Testing biological actions of medicinal plants from northern Vietnam on zebrafish embryos and larvae: Developmental, behavioral, and putative therapeutical effects. |
| title_short | Testing biological actions of medicinal plants from northern Vietnam on zebrafish embryos and larvae: Developmental, behavioral, and putative therapeutical effects. |
| title_sort | testing biological actions of medicinal plants from northern vietnam on zebrafish embryos and larvae developmental behavioral and putative therapeutical effects |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0294048&type=printable |
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