Urine Test Strip Quantitative Assay with a Smartphone Camera
Urine test strips for urinalysis are a common diagnostic tool with minimal costs and are used in various situations including homecare and hospitalization. The coloration scaled by the naked eye is simple, but it is suitable for semiquantitative analysis only. In this paper, a colorimetric assay is...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2024-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2024/6004970 |
| _version_ | 1797245048808538112 |
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| author | Miroslav Pohanka Jitka Zakova |
| author_facet | Miroslav Pohanka Jitka Zakova |
| author_sort | Miroslav Pohanka |
| collection | DOAJ |
| description | Urine test strips for urinalysis are a common diagnostic tool with minimal costs and are used in various situations including homecare and hospitalization. The coloration scaled by the naked eye is simple, but it is suitable for semiquantitative analysis only. In this paper, a colorimetric assay is developed based on a smartphone digital camera and urine test strips. Assays of pH, albumin, glucose, and lipase activity were performed as a tool for the diagnosis of aciduria, alkaluria, glycosuria, proteinuria, and leukocyturia. The RGB color channels were analyzed in the colorimetric assay, and the assay exerted good sensitivity, and all the particular diagnoses proved to be reliable. The limits of detection for glucose (0.11 mmol/L), albumin (0.15 g/L), and lipase (2.50 U/μL) were low enough to cover the expected physiological concentration, and the range for pH was also satisfactory. The urine test strips with a camera as an output detector proved applicability to spiked urine samples, and the results were also well in comparison to the standard assays which confirms the practical relevance of the presented findings. |
| first_indexed | 2024-04-24T19:20:43Z |
| format | Article |
| id | doaj.art-62f9daffa13341efbcc681465d55a1f2 |
| institution | Directory Open Access Journal |
| issn | 1687-8779 |
| language | English |
| last_indexed | 2024-04-24T19:20:43Z |
| publishDate | 2024-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj.art-62f9daffa13341efbcc681465d55a1f22024-03-26T00:00:03ZengHindawi LimitedInternational Journal of Analytical Chemistry1687-87792024-01-01202410.1155/2024/6004970Urine Test Strip Quantitative Assay with a Smartphone CameraMiroslav Pohanka0Jitka Zakova1Military Faculty of MedicineMilitary Faculty of MedicineUrine test strips for urinalysis are a common diagnostic tool with minimal costs and are used in various situations including homecare and hospitalization. The coloration scaled by the naked eye is simple, but it is suitable for semiquantitative analysis only. In this paper, a colorimetric assay is developed based on a smartphone digital camera and urine test strips. Assays of pH, albumin, glucose, and lipase activity were performed as a tool for the diagnosis of aciduria, alkaluria, glycosuria, proteinuria, and leukocyturia. The RGB color channels were analyzed in the colorimetric assay, and the assay exerted good sensitivity, and all the particular diagnoses proved to be reliable. The limits of detection for glucose (0.11 mmol/L), albumin (0.15 g/L), and lipase (2.50 U/μL) were low enough to cover the expected physiological concentration, and the range for pH was also satisfactory. The urine test strips with a camera as an output detector proved applicability to spiked urine samples, and the results were also well in comparison to the standard assays which confirms the practical relevance of the presented findings.http://dx.doi.org/10.1155/2024/6004970 |
| spellingShingle | Miroslav Pohanka Jitka Zakova Urine Test Strip Quantitative Assay with a Smartphone Camera International Journal of Analytical Chemistry |
| title | Urine Test Strip Quantitative Assay with a Smartphone Camera |
| title_full | Urine Test Strip Quantitative Assay with a Smartphone Camera |
| title_fullStr | Urine Test Strip Quantitative Assay with a Smartphone Camera |
| title_full_unstemmed | Urine Test Strip Quantitative Assay with a Smartphone Camera |
| title_short | Urine Test Strip Quantitative Assay with a Smartphone Camera |
| title_sort | urine test strip quantitative assay with a smartphone camera |
| url | http://dx.doi.org/10.1155/2024/6004970 |
| work_keys_str_mv | AT miroslavpohanka urineteststripquantitativeassaywithasmartphonecamera AT jitkazakova urineteststripquantitativeassaywithasmartphonecamera |